Aminated Poly(glycidyl methacrylate)s for Constructing Efficient Gene Carriers

Abstract

Aminated poly(glycidyl methacrylate) (PGMA) vectors could efficiently mediate gene delivery. Recently, we reported that ethanolamine (EA)-functionalized PGMA could provide high transfection efficiency, while exhibiting very low toxicity. Herein, different amine species, including 1-amino-2-propanol (AP1), 3-amino-2-propanol (AP2), EA, and N,N,-dimethylethylenediamine (DED), and its quaternized DED, were proposed to aminate PGMA. The DNA condensation abilities, pH buffering capacities, cytotoxicities, and gene transfection efficiencies of the resultant aminated PGMA vectors were systematically compared. Compared with EA, AP1 (or AP2) contains an additional methyl (or methylene) group. EA-, AP1-, and AP2-functionalized PGMA vectors exhibited similar condensation abilities. The methyl (from AP1) and methylene (from AP2) species could benefit the gene delivery. The transfection performance mediated by AP1-functionalized PGMA is best. DED possesses a tertiary amine group, which could be quaternized to further enhance the DNA condensation ability of aminated PGMA. No obvious increase in cytotoxicity of quaternized DED-aminated PGMA was observed. But both DED- and its quaternized counterpart-functionalized PGMA vectors exhibited very low pH buffering capacities, making them exhibit poor gene transfection performances. The current study would provide useful information for constructing better PGMA-based delivery systems with good biophysical properties.

One simple and stable coating of mixed‐charge copolymers on poly(vinyl chloride) films to improve antifouling efficiency

“…We selected GMA as the comonomer to tether the PSTG copolymers onto the surface of the PVC films. The epoxy groups of GMA possess high reactivities toward amino groups . In this study, the obtained PSTG polymers had both epoxy groups distributed randomly along the backbone and were grafted onto the amino‐functionalized PVC surface simply through the soaking of the films in PSTG solutions at 60 °C for 2 h. The grafting density of the PSTG copolymers (micrograms per square centimeter) as a function of the copolymer concentration was assessed through measurement of the weight increase of the films per unit surface area.…”

Post-polymerization modification reactions of poly(glycidyl methacrylate)s

“…[63][64][65][66][67] In some cases, the tertiary amines were further quaternised through the use of an alkyl halide to give access to side-chain cationic polymers. 68,69 In most cases so far, atom transfer radical polymerization (ATRP) was used for the preparation of the PGMA scaffold. Haddleton and co-workers showed that synthesis by a cobaltcatalysed chain transfer polymerisation could lead to the polymer chains displaying a methacrylate chain-end.…”

Smart Delivery Systems Based on Poly(glycidyl methacrylate)s‐Coated Organic/Inorganic Core–Shell Nanohybrids

“…For example: various cationic PGMAs have been obtained as carriers for anionic biomolecules, especially plasmid DNA (pDNA); fluorescent PGMAs have been fabricated by modifying with conventional chromophores (e.g., pyrene) or aggregation induced emission chromophores (e.g., tetraphenylethylene); stimuli‐responsive or targeting groups (folate, azobenzene, ferrocene, cyclodextrins, disulfide bond, etc.) have also been introduced for targeted and controlled delivery . Benefiting from the advances of polymer science and the diversified functionality of PGMAs, a series of PGMAs‐based nanoassemblies, such as nanoparticles, micelles (spherical, cylindric, wormlike, etc.…”