Amiloride inhibits murine erythroleukemia cell differentiation: evidence for a Ca2+ requirement for commitment.

Levenson, Robert; Housman, David E.; Cantley, Lewis C.

doi:10.1073/pnas.77.10.5948

Cited by 91 publications

(42 citation statements)

References 27 publications

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“…Evidence implicating calcium in control of erythroid growth and differentiation includes: (a) enhancement of Epo-induced murine erythroid colony growth by the ionophore A23187 and inhibition by treatment with EGTA, a nonspecific chelator of calcium (7); (b) demonstration that an increase in Ca 2ϩ influx is an early and necessary step in the commitment to differentiation of murine erythroleukemia cells (8 -10); and (c) the significant rise in [Ca] i stimulated by Epo observed at specific stages of human BFU-E differentiation (5). Substantial evidence supports the conclusion that erythropoietin stimulates calcium influx in erythroid cells through voltage-independent calcium-permeable channel(s) (8,10,(11)(12)(13). In patch clamp studies of human erythroid progenitor-derived cells, Epo stimulation increased calcium channel mean open time 2.5-fold and open probability 10-fold (13).…”

mentioning

confidence: 71%

“…Lines Using RT-PCR-The ability of erythropoietin to stimulate calcium influx in murine erythroid cells and erythroleukemia cell lines has been demonstrated previously (7)(8)(9)(10)(11)23). Here, to explore whether this influx occurred through the classical TRP channels, the expression of TRPC1 to -6 was examined in HCD-57 murine erythroleukemia cells and in Ba/F3 Epo-R cells, a hematopoietic cell line stably transfected with murine Epo-R and previously shown to respond to Epo with a rise in [Ca] i (23).…”

Section: Expression Of Trpc In Murine Tissues and Erythroid Cellmentioning

confidence: 99%

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Erythropoietin Modulates Calcium Influx through TRPC2

Chu¹,

Cheung

Barber

et al. 2002

Journal of Biological Chemistry

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mentioning

confidence: 71%

Section: Expression Of Trpc In Murine Tissues and Erythroid Cellmentioning

confidence: 99%

Erythropoietin Modulates Calcium Influx through TRPC2

Chu¹,

Cheung

Barber

et al. 2002

Journal of Biological Chemistry

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“…DMSO has effects on a large number of other biological systems and has been extensively studied as an inducer of differentiation of MEL cells (6,7). Recent evidence with the MEL cells suggests that a rise in cytoplasmic Ca-is a consequence of DMSO treatment and is a necessary but insufficient condition for MEL differentiation (4,14,29 …”

Section: Discussionmentioning

confidence: 99%

Induced muscle differentiation in an embryonal carcinoma cell line.

Edwards¹,

Harris²,

McBurney³

1983

Mol. Cell. Biol.

174

107

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Cells of the teratocarcinoma-derived line P19S1801A1 (01A1) are pluripotent embryonal carcinoma cells and can be induced to differentiate when aggregated and exposed to dimethyl sulfoxide. Many nonneural cell types appear in dimethyl sulfoxide-treated cultures, cardiac and skeletal muscle being the most easily identified. We have used immunofluorescence procedures with monoclonal antibodies directed against muscle myosin to confirm and quantitate the number of muscle cells formed. A monoclonal antibody reactive with an embryonal carcinoma-specific surface antigen was used to confirm the disappearance of undifferentiated cells after dimethyl sulfoxide treatment. Cardiac muscle cells developed within 4 to 5 days of drug exposure, but skeletal muscle cells did not become evident until 7 to 8 days. We have isolated a mutant cell line (D3) which appears to be incapable of muscle development but which does form neurons and glial cells when exposed to high retinoic acid concentrations. We propose that this system will be useful for investigation of the means by which pluripotent cells become committed to development along the striated muscle lineages.

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“…Moreover, reversal of cellular Ca2+ uptake inhibition by Ca2+ ionophore A23187 stimulated Me2SO-treated MEL cells to resume differentiating. This finding suggests involvement of the Na+/ Ca2+ antiport system in MEL cell differentiation (18,19).…”

mentioning

confidence: 84%

DNA fragments in Friend erythroleukemia cells induced by dimethyl sulfoxide.

Pulito¹,

Miller²,

Sassa³

et al. 1983

Proc. Natl. Acad. Sci. U.S.A.

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The idea of the correlation between DNA damage caused by dimethyl sulfoxide (Me2SO) and the induction of erythrodifferentiation in murine erythroleukemia (MEL) cells needs reevaluation as a result of the hydroxyapatite chromatographic analysis of DNA from shown to alter transport systems in the plasma membrane (4-6) in addition to affecting chromatin (7-9).When studied by alkaline sucrose gradient analysis, induction of MEL cells with Me2SO seems to cause a continuous degradation of cellular DNA (7,8). In previous studies two peaks were observed in the gradients, sedimenting either at 300 S and 120 S (8) or at 140 S and 17 S (7). The minor (slow sedimenting) fraction rose in Me2SO-treated cells relative to untreated control cultures. In one study (8) the cellular DNA was totally converted to the smaller component after 64-hr exposure to Me2SO. In another study (7), the ratio of DNA in the minor peak of Me2SO-treated cells relative to control cells rose to 2.85 by 12 hr and then slowly decreased, approaching 1 after 50 hr. The large increase in low molecular weight DNA was not seen in neutral-pH sucrose gradient sedimentation; therefore, the fragments must be generated by single-strand breaks.If Me2SO causes single-strand breaks in DNA, it is possible that both classes of inducers work by similar mechanisms to activate chromatin by DNA cleavage. On the other hand, if the analytical procedure for determining the presence of single-strand breaks were subject to an unrecognized artifact, DNA cleavage might not be involved in MEL cell differentiation. Because this information is central to further studies of gene activation in this system, we reinvestigated this question.The measurement of DNA nicking by alkaline sucrose gradient sedimentation can be attended by serious artifacts (10), especially when applied to high molecular weight mammalian DNA, which may not completely separate into single strands. We undertook to examine the accumulation of single-strand breaks by an alternative procedure, alkaline hydroxyapatite column chromatography, which has been successfully used by others (11, 12) An alkaline separation technique combined with hydroxyapatite chromatography (10, 11) has been used to detect DNA breaks induced by Me2SO. Between 1 and 2 X 105 cells were removed, centrifuged, washed, and suspended in 50 A.l of saline/EDTA (150 mM NaCl/1 mM EDTA, pH 7.5) solution. To this, 1 ml of alkali (30 mM NaOH/10 mM Na2HPO4/300 mM NaCl, pH 12.6) was added, and the mixture was kept at 20°C for 25 min in the dark, neutralized with addition of 10 mM NaH2PO4 containing phenol red as pH indicator, transferred into an ice-water bath, and sonicated for 15 sec. After sonication, sodium dodecyl sulfate was added to a final concentration of 0.25%.Hydroxyapatite Column Chromatography. A water-jacketed glass column (1 x 25 cm) was packed with 250 mg of hydroxyapatite (DNA grade Bio-Gel HTP) and maintained at 60°C. Elution buffers used were 0.01 M sodium phosphate (pH 6.8) for washing and 0.13 and 0.25 M potassium phosphate...

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Amiloride inhibits murine erythroleukemia cell differentiation: evidence for a Ca2+ requirement for commitment.

Cited by 91 publications

References 27 publications

Erythropoietin Modulates Calcium Influx through TRPC2

Erythropoietin Modulates Calcium Influx through TRPC2

Induced muscle differentiation in an embryonal carcinoma cell line.

DNA fragments in Friend erythroleukemia cells induced by dimethyl sulfoxide.

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