Amifostine-conjugated pH-sensitive calcium phosphate-covered magnetic-amphiphilic gelatin nanoparticles for controlled intracellular dual drug release for dual-targeting in HER-2-overexpressing breast cancer

Li, Wei Ming; Chiang, Chih Sheng; Huang, Wen Chang; Su, Chia Wei; Chiang, Min Yu; Chen, Jian Yi

doi:10.1016/j.jconrel.2015.10.020

Cited by 53 publications

(24 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An in vitro assay based on the tetrazolium-salt addressing cells viability was chosen, being currently used in similar studies [22,33]. The cytotoxic effect was characterized by comparison with two other experimental conditions of cells: magnetite nanoparticles (NP) and treatment with Gemcitabine alone (GEM) for three periods of cell treatment (24 h, 48 h and 72 h).…”

Section: Resultsmentioning

confidence: 99%

Fabrication and Cytotoxicity of Gemcitabine-Functionalized Magnetite Nanoparticles

et al. 2017

View full text Add to dashboard Cite

Nanotechnology has been successfully used for the fabrication of targeted anti-cancer drug carriers. This study aimed to obtain Fe3O4 nanoparticles functionalized with Gemcitabine to improve the cytotoxic effects of the chemotherapeutic substance on cancer cells. The (un) functionalized magnetite nanoparticles were synthesized using a modified co-precipitation method. The nanoconjugate characterization was performed by XRD, SEM, SAED and HRTEM; the functionalizing of magnetite with anti-tumor substances has been highlighted through TGA. The interaction with biologic media has been studied by means of stability and agglomeration tendency (using DLS and Zeta Potential); also, the release kinetics of the drug in culture media was evaluated. Cytotoxicity of free-Gemcitabine and the obtained nanoconjugate were evaluated on human BT 474 breast ductal carcinoma, HepG2 hepatocellular carcinoma and MG 63 osteosarcoma cells by MTS. In parallel, cellular morphology of these cells were examined through fluorescence microscopy and SEM. The localization of the nanoparticles related to the cells was studied using SEM, EDX and TEM. Hemolysis assay showed no damage of erythrocytes. Additionally, an in vivo biodistribution study was made for tracking where Fe3O4@Gemcitabine traveled in the body of mice. Our results showed that the transport of the drug improves the cytotoxic effects in comparison with the one produced by free Gemcitabine for the BT474 and HepG2 cells. The in vivo biodistribution test proved nanoparticle accumulation in the vital organs, with the exception of spleen, where black-brown deposits have been found. These results indicate that our Gemcitabine-functionalized nanoparticles are a promising targeted system for applications in cancer therapy.

show abstract

Section: Resultsmentioning

confidence: 99%

Fabrication and Cytotoxicity of Gemcitabine-Functionalized Magnetite Nanoparticles

et al. 2017

View full text Add to dashboard Cite

show abstract

“…These risks can be omitted when magnetic targeting of MNP is intravenously applied, however, at the cost of reaching comparatively low MNP concentrations of approx. 150 µg(Fe)/g(Tumor) (3 mM) 26 to 400 µg(Fe)/g(Tumor) (7 mM) 27 . Such low concentrations were achieved for a mouse tumor model using permanent magnets.…”

Section: Introductionmentioning

confidence: 99%

Combining Bulk Temperature and Nanoheating Enables Advanced Magnetic Fluid Hyperthermia Efficacy on Pancreatic Tumor Cells

Engelmann

Roeth

Eberbeck

et al. 2018

Sci Rep

View full text Add to dashboard Cite

Many efforts are made worldwide to establish magnetic fluid hyperthermia (MFH) as a treatment for organ-confined tumors. However, translation to clinical application hardly succeeds as it still lacks of understanding the mechanisms determining MFH cytotoxic effects. Here, we investigate the intracellular MFH efficacy with respect to different parameters and assess the intracellular cytotoxic effects in detail. For this, MiaPaCa-2 human pancreatic tumor cells and L929 murine fibroblasts were loaded with iron-oxide magnetic nanoparticles (MNP) and exposed to MFH for either 30 min or 90 min. The resulting cytotoxic effects were assessed via clonogenic assay. Our results demonstrate that cell damage depends not only on the obvious parameters bulk temperature and duration of treatment, but most importantly on cell type and thermal energy deposited per cell during MFH treatment. Tumor cell death of 95% was achieved by depositing an intracellular total thermal energy with about 50% margin to damage of healthy cells. This is attributed to combined intracellular nanoheating and extracellular bulk heating. Tumor cell damage of up to 86% was observed for MFH treatment without perceptible bulk temperature rise. Effective heating decreased by up to 65% after MNP were internalized inside cells.

show abstract

“…Compared to the single-drug chemotherapy, the proper drug combination, especially the combination of cytotoxic and chemosensitizing agents exhibited better synergistic actions and target selectivity, more effective for tumor localization, thus overcoming multi-drug resistance (MDR) (1)(2)(3). For instance, the combination of anticancer agents such doxorubicin (DOX) and curcumin (CUR) has been shown to modulate different signaling pathways in breast cancer cells, which are beneficial to reverse multidrug resistance (MDR) (4,5).…”

Section: Introductionmentioning

confidence: 99%

Co-delivery of doxorubicin and pH-sensitive curcumin prodrug by transferrin-targeted nanoparticles for breast cancer treatment

2017

View full text Add to dashboard Cite

The natural product curcumin and the chemotherapeutic agent doxorubicin have been used in the treatment of many cancers, including breast cancer. However, fast clearance and unspecific distribution in the body after intravenous injection are still challenges to be overcome by an ideal nano-sized drug delivery system in cancer treatment. In this study we design transferrin (Tf) decorated nanoparticles (NPs) to co-deliver CUR and DOX for breast cancer treatment. A pH-sensitive prodrug, transferrin-poly(ethylene glycol)-curcumin (Tf-PEG-CUR), was synthesized and used for the self‑assembling of NPs (Tf-PEG-CUR NPs). DOX is incorporated into the Tf-PEG-CUR NPs to obtain Tf-PEG-CUR/DOX NPs. In vitro cytotoxicity studies and in vivo antitumor activity were carried out using MCF-7 cells and mice bearing MCF-7 cells, respectively. Tf-PEG-CUR/DOX NPs has a particle size of 89 nm and a zeta potential of -15.6 mV. This system displayed remarkably higher efficiency than other systems both in vitro and in vivo. DOX and CUR were successfully loaded into nanocarriers. The in vitro cell viability assays revealed the combination of Tf-PEG-CUR and DOX NPs exhibited higher cytotoxicity in vitro in MCF-7 cells compared with Tf-PEG-CUR NPs alone. Using the breast cancer xenograft mouse model, we demonstrate that this co-encapsulation approach resulted in an efficient tumor-targeted drug delivery, decreased cytotoxic effects and exhibited stronger antitumor effect.

show abstract

Amifostine-conjugated pH-sensitive calcium phosphate-covered magnetic-amphiphilic gelatin nanoparticles for controlled intracellular dual drug release for dual-targeting in HER-2-overexpressing breast cancer

Cited by 53 publications

References 52 publications

Fabrication and Cytotoxicity of Gemcitabine-Functionalized Magnetite Nanoparticles

Fabrication and Cytotoxicity of Gemcitabine-Functionalized Magnetite Nanoparticles

Combining Bulk Temperature and Nanoheating Enables Advanced Magnetic Fluid Hyperthermia Efficacy on Pancreatic Tumor Cells

Co-delivery of doxorubicin and pH-sensitive curcumin prodrug by transferrin-targeted nanoparticles for breast cancer treatment

Contact Info

Product

Resources

About