Amido Black 10B a widely used azo dye causes DNA damage in pro- and eukaryotic indicator cells

Brambilla, Crislaine Maria Carvalho da Cruz; Garcia, Ana Letícia Hilário; Silva, Fernanda Rabaioli da; Taffarel, Sílvio Roberto; Grivicich, Ivana; Picada, Jaqueline Nascimento; Scotti, Amanda Souza; Dalberto, Daiana; Mišík, Miroslav; Knasmüller, Siegfried; Silva, Juliana da

doi:10.1016/j.chemosphere.2018.11.026

Cited by 32 publications

(4 citation statements)

References 34 publications

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“…Fenton reagent is a solution of Fe 2þ /Fe 3þ and hydrogen peroxide (H 2 O 2 ) which can rapidly oxidize diverse organic contaminants by generating highly reactive hydroxyl radicals ( OH) [25]. Among the organic pollutants, azo dyes are of particular concern, as the extensive application of these carcinogenic compounds in different industries has led to their resistance against biological and physicochemical treatments [26,27,28,29,30,31], making the conventional treatment methods ineffective [32]. Various techniques such as hydrothermal [33], sol-gel process [13], chemical co-precipitation [34], template-assisted method [35], thermal decomposition [36], and vapor-solid growth techniques [37] have been exploited for the synthesis of INPs, each suffering from several drawbacks, including a) toxic by-products whose removal requires special treatments, b) high temperature, c) the need for substrate or templates necessitating pre-manufacturing and post-removal difficulties, and d) contaminants which may cause further ecological damages [38,39].…”

Section: Introductionmentioning

confidence: 99%

Green synthesis of iron-based nanoparticles using Chlorophytum comosum leaf extract: methyl orange dye degradation and antimicrobial properties

Ardakani

Alimardani

Tamaddon

et al. 2021

Heliyon

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Nowadays, green synthesis methods have gained growing attention in nanotechnology owning to their versatile features including high efficiency, cost-effectiveness, and eco-friendliness. Here, the aqueous extract of Chlorophytum comosum leaf was applied for the preparation of iron nanoparticles (INPs) to obtain spherical and amorphous INPs with a particle size below 100 nm as confirmed by TEM. The synthesized INPs managed to eliminate methyl orange (MO) from the aqueous solution. The concentration of MO can be easily checked via ultraviolet-visible (UV-Vis) spectroscopy throughout the usage of INPs at the presence of H 2 O 2 . The synthesized INPs exhibited MO degradation efficiency of 77% after 6 h. Furthermore, the synthesized INPs exhibited antibacterial activity against both Gram-negative and Gram-positive bacteria. The prepared INPs have an impressive effect on Staphylococcus aureus at concentrations below 6 μg/ml. Overall, the synthesized INPs could considerably contribute to our combat against organic dyes and bacteria.

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Section: Introductionmentioning

confidence: 99%

Green synthesis of iron-based nanoparticles using Chlorophytum comosum leaf extract: methyl orange dye degradation and antimicrobial properties

Ardakani

Alimardani

Tamaddon

et al. 2021

Heliyon

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“…In our genotoxic investigation of Arianor Ebony dye, the comet assay and MN test revealed significant effects in all tested concentrations. Brambilla et al (38), investigating the genotoxic potential of an azo dye in the same cell line, also found positive results in both comet and MN tests. These authors concluded that the DNA strand breaks leading to comet formation were only partly repaired and induced persisting chromosomal alterations detectable as MN, which could be an explanation for the results obtained in the present research.…”

Section: Discussionmentioning

confidence: 87%

Toxic effects of Arianor Ebony hair dye on human cells

Tafurt-Cardona¹,

Suares-Rocha

Silva

et al. 2023

Braz J Med Biol Res

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To evaluate the risks of hair dye exposure, we investigated cellular and molecular effects of Arianor Ebony dye, which is a mixture of azo and anthraquinone dyes, used in the composition of the black color. Cytotoxicity, genotoxicity, and gene expression of relevant molecules of apoptotic and oxidative stress mechanisms were investigated in HepG2 cells exposed to Arianor Ebony. Results showed that the dye did not induce cytotoxicity to exposed cells at a concentration up to 50 mg/mL compared to the negative control. However, genotoxic assays indicated that the dye was able to damage the genetic material at a concentration of 25 mg/mL, with induction factor values of exposed cells two-to five-fold higher than those recorded for the negative control. Moreover, the lowest observed effect concentration was 12.5 mg/mL. For gene expression, relevant changes were observed in cytochrome c and caspase 9, which decreased in cells incubated with the dye in a dose-dependent manner when compared with the negative control. In parallel, the expression of genes for antioxidant enzymes was increased in exposed cells, suggesting the presence of metabolic routes that protect cells against the toxic effect of the dye, avoiding exacerbated cellular death. Results suggested that the dye disrupted cellular homeostasis through mitochondrial dysfunction, which may be hazardous to human health. Thus, further investigations are necessary to deeply understand the mechanisms of action of the dye, considering its toxic potential found in our ex vivo assays.

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“…Water pollution by dyes, is a serious risk for the environment and human health owing to their toxicity and mutagenic effects causing damage to genetic material and possibly cancer [1,2]. So eliminate these pollutants is an important and demanding task for the researchers.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of the Spinel Znfe2O4 Application to Photodegradation of Organic Pollutant Under Visible Light

2023

AICAST-23, ACABE-23 &Amp; AICHSS-23 July 4-6, 2023 Athens (Greece)

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The spinel ZnFe 2 O 4 , prepared by co-precipitation method with NaOH as a precipitating agent, was used for photo-degradation of Safranine O, recalcitrant dey. The textural, optical and electrochemical properties of the as-prepared ZnFe 2 O 4 were investigated. The X-ray diffraction shows a single spinel cubic phase with a good crystallinity. The specific surface is around of 57.67 m 2 /g. The TEM results showed that ZnFe 2 O 4 nanopowders are mainly composed of irregular particles. Fourier transform infrared spectroscopy (FTIR) demonstrated the presence of Fe-O and Zn-O bonds in the range of 300 cm -1 to 700 cm -1 . The diffuse reflectance gives a band gap of 1.64 eV, properly matched to the sun spectrum. As application, the photo-degradation of Safranine O was successfully realized yielding a depollution parentage of 36 % within 120 min for an initial concentration of 10 mg L −1 . A photocatalytic degradation mechanism occurred through (electron-hole) pairs generation.

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Amido Black 10B a widely used azo dye causes DNA damage in pro- and eukaryotic indicator cells

Cited by 32 publications

References 34 publications

Green synthesis of iron-based nanoparticles using Chlorophytum comosum leaf extract: methyl orange dye degradation and antimicrobial properties

Green synthesis of iron-based nanoparticles using Chlorophytum comosum leaf extract: methyl orange dye degradation and antimicrobial properties

Toxic effects of Arianor Ebony hair dye on human cells

Synthesis and Characterization of the Spinel Znfe2O4 Application to Photodegradation of Organic Pollutant Under Visible Light

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