American tegumentary leishmaniasis: mRNA expression for Th1 and Treg mediators are predominant in patients with recent active disease

Souza, M. A.; Almeida, Thays Miranda de; Castro, Maria Carolina Accioly Brelaz de; Oliveira-Mendes, Andresa P.; Almeida, André; Oliveira, Beatriz Coutinho de; Rocha, Lucas Ferreira da; Medeiros, Ângela Cristina Rapela; Brito, Maria Edileuza Felinto de; Dessein, Alain; Pereira, Valéria Rêgo Alves

doi:10.1016/j.imbio.2015.08.009

Cited by 8 publications

(6 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cellular immunity is critical in ACL, because Th1 and Th2 cytokines are important in hindering development of the infection . Other T‐cell subtypes such as Th17 participate in this event, although the exact role of each is not well established . Such studies were based on analysing blood cells .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

American cutaneous leishmaniasis: In situ immune response of patients with recent and late lesions

Gomes

Martines

Kanamura

et al. 2017

Parasite Immunology

View full text Add to dashboard Cite

TNF-α, IFN-γ, IL-10, IL-17, CD68 and CD57 were evaluated in biopsies of patients with American cutaneous leishmaniasis living in Sorocaba, Brazil. The analyses were performed considering the time of lesions from 23 patients with recent lesions (Group I) and 19 patients with late lesions (Group II). All patients were infected with Leishmania (Viannia) braziliensis. Immunostaining cells for CD68, CD57, TNF- α, IFN-γ, IL-10 and IL-17 were performed by immunohistochemistry. Except for CD68 and IL-17, the distribution of in situ for CD57, IL-10, TNF-α and IFN-γ showed that patients with recent lesions expressed higher levels than those with late lesions. The comparison of cytokine expression/group showed that IL-10 was significantly higher than IL-17 and IFN-γ (similar data were shown in IL-17 compared with TNF-α), suggesting an immunological balance between inflammatory-anti-inflammatory agents. This balance was similar for two groups of patients. In conclusion, these data suggested that (i) patients from Group I had recent lesions (in the beginning of chronic phase) compared to those from Group II and (ii) the modulation of inflammatory response in patients with recent American cutaneous leishmaniasis was correlated with IL-10 expression in skin lesions preventing the development of mucosal forms. The parasite treatment also prevented the evolution of severe forms.

show abstract

Section: Discussionmentioning

confidence: 99%

“…subtypes such as Th17 participate in this event, although the exact role of each is not well established. 13,21,32 Such studies were based on analysing blood cells. 10,21,32,33 This study analysed the specific immune response at the site of the infected lesion considered an important tool for defining the evolution of the infection.…”

Section: Ifn-γ Il-17mentioning

confidence: 99%

American cutaneous leishmaniasis: In situ immune response of patients with recent and late lesions

Gomes

Martines

Kanamura

et al. 2017

Parasite Immunology

View full text Add to dashboard Cite

show abstract

“…This response has been associated with disease control, macrophage activation, and parasite elimination from the host (11–13). Our previous data show that cells from patients infected with L. braziliensis produce high amounts of IFN-γ after stimulation with whole lysed parasite (14, 15). To initiate cellular response, dendritic cells (DCs), which are specialized antigen-presenting cells (APCs), have the unique capacity of priming naive T cells by presenting peptide antigens bound to major histocompatibility proteins (MHCs), co-stimulating and secreting cytokines, and thus mounting a T cell response against Leishmania spp.…”

Section: Introductionmentioning

confidence: 99%

Combination of In Silico Methods in the Search for Potential CD4+ and CD8+ T Cell Epitopes in the Proteome of Leishmania braziliensis

et al. 2016

View full text Add to dashboard Cite

The leishmaniases are neglected tropical diseases widespread throughout the globe, which are caused by protozoans from the genus Leishmania and are transmitted by infected phlebotomine flies. The development of a safe and effective vaccine against these diseases has been seen as the best alternative to control and reduce the number of cases. To support vaccine development, this work has applied an in silico approach to search for high potential peptide epitopes able to bind to different major histocompatibility complex Class I and Class II (MHC I and MHC II) molecules from different human populations. First, the predicted proteome of Leishmania braziliensis was compared and analyzed by modern linear programs to find epitopes with the capacity to trigger an immune response. This approach resulted in thousands of epitopes derived from 8,000 proteins conserved among different Leishmania species. Epitopes from proteins similar to those found in host species were excluded, and epitopes from proteins conserved between different Leishmania species and belonging to surface proteins were preferentially selected. The resulting epitopes were then clustered, to avoid redundancies, resulting in a total of 230 individual epitopes for MHC I and 2,319 for MHC II. These were used for molecular modeling and docking with MHC structures retrieved from the Protein Data Bank. Molecular docking then ranked epitopes based on their predicted binding affinity to both MHC I and II. Peptides corresponding to the top 10 ranked epitopes were synthesized and evaluated in vitro for their capacity to stimulate peripheral blood mononuclear cells (PBMC) from post-treated cutaneous leishmaniasis patients, with PBMC from healthy donors used as control. From the 10 peptides tested, 50% showed to be immunogenic and capable to stimulate the proliferation of lymphocytes from recovered individuals.

show abstract

“…We juxtaposed the cytokines produced in the presence of SLA and found that CXCL‐11, IL‐17a, as well as IFN‐ γ significantly differed between the non‐healing and healing groups (Tables and Figs. ), suggesting that different cytokines produced by the TH1 subset decreased the duration of Leishmania ‐induced lesions .…”

Section: Discussionmentioning

confidence: 59%

Comparison of pro‐inflammatory cytokines of non‐healing and healing cutaneous leishmaniasis

et al. 2017

View full text Add to dashboard Cite

Cutaneous leishmaniasis (CL) heals spontaneously within several weeks or months, but, in rare cases, CL-active lesions last for many years. In this study, we assessed cell-mediated immunity in non-healing CL through the measurement of three pro-inflammatory cytokines: Interferon-γ (IFN-γ), IL-17a and CXCL-11. For this, 32 patients afflicted with healing or non-healing CL were recruited in this study. Peripheral blood mononuclear cells (PBMCs) of every patient were treated with three antigens: purified protein derivative (PPD), soluble Leishmania antigen (SLA) and phytohaemagglutinin (PHA). Cytokine quantification was performed using enzyme-linked immunosorbent assay (ELISA) method. Results of our study showed that neither cytokine produced in the presence of a PPD stimulator (as an irrelevant antigen) significantly differed between the healing and non-healing groups (P-value ≥0.05 for all of them). However, IFN-γ, CXCL-11 and IL-17a levels produced in the presence of PHA or SLA were significantly higher within the healing than in the non-healing group (P-value <0.01 for all of them). It seems that appropriate levels of IFN-γ, as well as IL-17a and CXCL-11, contribute to the control of Leishmania infection.

show abstract

American tegumentary leishmaniasis: mRNA expression for Th1 and Treg mediators are predominant in patients with recent active disease

Cited by 8 publications

References 43 publications

American cutaneous leishmaniasis: In situ immune response of patients with recent and late lesions

American cutaneous leishmaniasis: In situ immune response of patients with recent and late lesions

Combination of In Silico Methods in the Search for Potential CD4+ and CD8+ T Cell Epitopes in the Proteome of Leishmania braziliensis

Comparison of pro‐inflammatory cytokines of non‐healing and healing cutaneous leishmaniasis

Contact Info

Product

Resources

About