American tegumentary leishmaniasis: antigen-gene polymorphism, taxonomy and clinical pleomorphism

García, Ana Lineth; Kindt, A.; Quispe-Tintaya, Kelly Wilber; Bermúdez, H.; Llanos, Alejandro; Arévalo, Jorge; Bañuls, Anne‐Laure; Doncker, Simonne De; Ray, D. Le; Dujardin, Jean‐Claude

doi:10.1016/j.meegid.2004.07.003

Cited by 76 publications

(72 citation statements)

References 29 publications

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“…Briefly, DNA was extracted from cultivated promastigotes with the QIAmp DNA mini Kit (Qiagen, Hilden, Germany), amplified and digested as reported elsewhere. [12][13][14] Restriction patterns were resolved by capillary electrophore- …”

Section: Methodsmentioning

confidence: 99%

The Sensitivity of Clinical Isolates of Leishmania From Peru and Nepal to Miltefosine

Yardley¹,

Croft²,

Doncker³

et al. 2005

The American Journal of Tropical Medicine and Hygiene

105

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Abstract. Clinical isolates of Leishmania, from visceral leishmaniasis (VL) cases in Nepal and from cutaneous leishmaniasis (CL) cases in Peru, were cultured using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to type species and strain. Promastigotes from 38 isolates, within eight passages from isolation, were used to infect mouse peritoneal macrophage cultures in vitro, and the amastigote sensitivity to miltefosine was determined. The concentration required to kill 50% of intracellular amastigotes from Nepalese VL isolates, all typed as Leishmania (L.) donovani (N ‫ס‬ 24) from both Sb v responders and nonresponders, ranged from 8.7 to 0.04 g/mL. In contrast, the concentration required to kill 50% intracellular amastigotes from isolates from Peru, typed as L. (V.) and L. (V.) lainsoni (N ‫ס‬ 4) was 3.4 to 1.9 g/mL. This demonstrates a notable difference in the intrinsic sensitivity of Leishmania species to miltefosine in vitro. If this model can be correlated to therapeutic outcome, it may have implications for the interpretation of clinical trials.

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Section: Methodsmentioning

confidence: 99%

The Sensitivity of Clinical Isolates of Leishmania From Peru and Nepal to Miltefosine

Yardley¹,

Croft²,

Doncker³

et al. 2005

The American Journal of Tropical Medicine and Hygiene

105

View full text Add to dashboard Cite

show abstract

“…Isolates were essentially obtained before treatment of patients, typed by PCR-RFLP analysis of hsp70 and cpb genes (Garcia et al 2005) and tested as intracellular amastigotes for their in vitro susceptibility to Sb V (Yardley et al 2006) (see Table 1 for summary of isolate features). Promastigote forms were grown at 24°C in a biphasic medium consisting of rabbit blood agar overlaid with medium 199 (M199; Sigma) containing 20% heat-inactivated fetal bovine serum (FBS; Lonza Bioscience), 25 mM Hepes (pH 7·4), 100 units/ml penicillin and 100 μg/ml streptomycin (Lonza).…”

Section: Parasites and In Vitro Culturementioning

confidence: 99%

Comparison of gene expression patterns amongLeishmania braziliensisclinical isolates showing a differentin vitrosusceptibility to pentavalent antimony

et al. 2010

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Evaluation of Leishmania drug susceptibility depends on in vitro Sb(V) susceptibility assays, which are labour-intensive and may give a biased view of the true parasite resistance. Molecular markers are urgently needed to improve and simplify the monitoring of Sb(V)-resistance. We analysed here the gene expression profile of 21 L. braziliensis clinical isolates in vitro defined as Sb(V)-resistant and -sensitive, in order to identify potential resistance markers

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“…The clinical manifestations depend on the parasite species, the vector and the host's genetic and immunological constitution. Although some Leishmania species may generate different clinical forms, these forms are usually associated with a single species 11 .…”

mentioning

confidence: 99%

Clinical, epidemiological and laboratory aspects of patients with American cutaneous leishmaniasis in the State of Pernambuco

Reis

et al. 2008

Rev. Soc. Bras. Med. Trop.

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The diagnosis for American cutaneous leishmaniasis is based on an association of clinical, epidemiological and laboratory characteristics. The present study identified the circulating species of Leishmania in the State of Pernambuco, described its clinical-epidemiological characteristics and diagnosed the disease. Nineteen patients presenting active lesions who had been diagnosed through clinical evaluation and laboratory tests were selected. The tests included direct investigation, in vitro culturing, Montenegro skin test, indirect immunofluorescence and polymerase chain reaction. The Montenegro Skin Test showed positive results in 89% of the patients; indirect immunofluorescence, in 79%; direct investigation, in 58%; and polymerase chain reaction in 75%. Seven Leishmania (Viannia) braziliensis samples were isolated from these patients and were characterized by means of specific monoclonal antibodies. These data confirm that a combination of different diagnosis techniques is needed in order to obtain efficient results and that, so far, Leishmania (Viannia) braziliensis is the only species responsible for American cutaneous leishmaniasis infection in Pernambuco. Thus, it is essential to identify the parasite species involved in cases of human disease in an endemic area in order to determine the clinical and epidemiological characteristics, especially with regard to diagnosis, therapy development and disease prognosis. Key-words:American cutaneous leishmaniasis. Leishmania (Viannia) braziliensis. Montenegro skin test. Indirect immunofluorescence. Polymerase chain reaction. RESUMOO diagnóstico da leishmaniose cutânea americana é baseado na associação dos aspectos clínicos, epidemiológicos e laboratoriais. O presente estudo identificou a espécie de Leishmania circulante no Estado de Pernambuco, descreveu os aspectos clínico-epidemiológicos e diagnosticou a doença. Foram selecionados 19 pacientes apresentando lesão ativa e diagnosticados através de avaliação clínica e pelos exames laboratoriais que incluíram a pesquisa direta, cultivo in vitro, intradermorreação de Montenegro, imunofluorescência indireta e reação em cadeia de polymerase. A intradermorreação de Montenegro apresentou resultado positivo em 89% dos pacientes. A imunofluorescência indireta apresentou 79% de positividade, a pesquisa direta apresentou 58% e a reação em cadeia de polymerase 75% de positividade. Sete amostras de Leishmania (Viannia) braziliensis foram isoladas desses pacientes e caracterizadas através de anticorpos monoclonais específicos. Esses dados confirmam que é necessário a combinação de diferentes técnicas diagnósticas para se obter resultados eficientes e que, até o momento, a Leishmania (Viannia) braziliensis é a única espécie responsável pela infecção da leishmaniose cutânea americana em Pernambuco. Portanto, a identificação da espécie do parasito envolvido nos casos da doença no homem em uma área endêmica é essencial para o conhecimento dos aspectos clínicos e epidemiológicos, sobretudo para o diagnóstico e para o desenvol...

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American tegumentary leishmaniasis: antigen-gene polymorphism, taxonomy and clinical pleomorphism

Cited by 76 publications

References 29 publications

The Sensitivity of Clinical Isolates of Leishmania From Peru and Nepal to Miltefosine

The Sensitivity of Clinical Isolates of Leishmania From Peru and Nepal to Miltefosine

Comparison of gene expression patterns amongLeishmania braziliensisclinical isolates showing a differentin vitrosusceptibility to pentavalent antimony

Clinical, epidemiological and laboratory aspects of patients with American cutaneous leishmaniasis in the State of Pernambuco

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