Ameliorative potential of vitamin C and E against Roundup‐glyphosate induced genotoxicity triggering apoptosis in caprine granulosa cells

Abstract

The present study was aimed to investigate the genotoxic and apoptotic effects of glyphosate (GLP) in Roundup formulation along with mitigation of two potent antioxidants that is, vitamin C and E in caprine granulosa cells in vitro. The entire work was done in a dose and time dependent manner where different concentrations of GLP (0.1, 2.0, and 4.0 mg/ml) in Roundup and antioxidants (0.5 and 1.0 mM) were employed to culture of granulosa cells for exposure durations of 24, 48, and 72 h. Analysis of GLP‐induced geno‐toxicity was accomplished by using single cell gel electrophoresis (comet assay) assay. Results have shown increased incidences of DNA fragmentation, evidenced by presence of different types of comets (Type 1–Type 4) in Roundup‐GLP‐ exposed groups in contrast to the control group (Type 0 comet). However, mitigation by both vitamin C and E was significant (p < .05) in combating the GLP‐induced genotoxicity in granulosa cells in a concentration‐ and time‐dependent manner. The results of our study provide a clear indication of the ameliorative actions of vitamin C and E against Roundup‐GLP‐induced genotoxicity that instigate apoptosis in ovarian granulosa cells of caprine.

Glyphosate Interference in Follicular Organization in the Wall Lizard Podarcis siculus

“…It is impossible to know if the newly recruited oocytes would have been ovulated because, in Podarcis, the maximum number of eggs is strictly controlled and depends on female size more than on environmental cues [ 68 , 70 ]. It is, however, quite interesting that no atresia was observed, though treatment lasted for 3 weeks, and glyphosate induces follicular granulosa degeneration [ 6 ]. In Podarcis, follicle selection occurs in early diplotene, before the formation of the primordial follicle [ 71 ], a convenient strategy for a small species that produces large and telolecithal eggs.…”

“…Gly has been found in human urine following its ingestion through contaminated vegetables [ 2 , 3 ]. Experimental exposure of mammals to Gly had mutagenic and carcinogenic effects [ 4 , 5 , 6 ], causing neurotoxicity, hepatotoxicity, and disruption of the reproductive axis [ 1 , 7 ]. As a consequence, fertility is greatly reduced by Gly; implantation failures [ 8 ] and teratogenesis [ 9 ] have been detected, as well as interferences with both spermatogenesis and oogenesis [ 10 ].…”

SIRT1 and antioxidants in infertile females: Exploration of the role of vitamin D

“…Glutathione is a family of enzymes that includes glutathione peroxidase (GPx), glutathione S-transferase (GST), and glutathione reductase (GSH) [ 18 ]. It is an endogenous antioxidant that protects the cell from OS and also preserves other antioxidants such as vitamins C and E; the role of these vitamins has been already documented [ 19 ]. Visfatin is an adipokine released from visceral adipose tissue dominantly [ 20 ].…”