Ameliorative effects of vitamin B17 on the kidney against Ehrlich ascites carcinoma induced renal toxicity in mice

Abstract

Cancer is the major cause of death and many factors that lead to its occurrences, such as environmental pollution and pesticides and other factors. Ehrlich carcinoma development depends on many things associated with the environment, nutrition, personal habits, and family history. The present study aimed to evaluate the potential protective effects of vitamin B17 (VB17) against Ehrlich ascites carcinoma (EAC) that induced kidney toxicity in female mice. The mice were divided into five groups (first group, control group; second group, VB17 group; third group, EAC group; fourth group, pretreated EAC with VB17; fifth group, cotreated EAC with VB17). Results showed the VB17 in pretreated (G4) and cotreated (G5) groups lead to an improvement in DNA damage and cytological examination, in addition significantly (P < .05) increase in Na+, red blood cell, hemoglobin, hematocrit value, mean corpuscular hemoglobin (MCH), and MCH concentration, whereas significantly (P < .05) decrease in urea, creatinine, K+, platelets, and white blood cells while insignificant (P < .05) changes in mean corpuscular volume when compared to the EAC group. Many histopathological changes were observed in kidney sections in EAC as marked damage and degenerated, glomerular atrophy, the Malpighian corpuscles that lost their characteristic configuration. On the other hand, a moderate improvement and arrangement in the kidney histological structure in pretreated VB17 + EAC, while a mild enhancement and arrangement of the kidney structure in cotreated EAC + VB17. In addition, depletion in renal P53 and PCNA protein expression compared with the EAC group. It could be concluded that VB17 has a potential renal protective effect against EAC cells induced kidney injury.

Genotoxic and antigenotoxic potential of amygdalin on isolated human lymphocytes by the comet assay

“…It was demonstrated that it significantly reduced (175 mg/Kg b.w/day, orally) the levels of tumor tissue in mice. The other two studies carried out by Mutar et al (2020) and Tousson et al (2020) revealed that pre-and simultaneous treatment of vitamin B17 (100 mg/kg b.w/day, intramuscularly) inhibited DNA damages in kidney and liver in the Ehrlich ascites carcinoma. It was suggested that Vitamin B17 had an ameliorative activity against Ehrlich ascites carcinoma-induced kidney and liver injury and toxicity in mice.…”

“…It has been reported that amygdalin, either administered intramuscularly (P1: 0.6 mg/kg; P2: 3 mg/kg) or the animals were fed with a mixture of commercial diet and apricot kernels (P3: 60 mg/kg; P4: 300 mg/kg), had no significant effect on the formation of aneuploidy in rabbit blood cells (Curlej, Halenar, Kolesarova, Curlejova, & Chrenek, 2016). Besides, Mutar, Tousson, Hafez, Abo Gazia, and Salem (2020) and Tousson, Hafez, Gazia, Salem, and Mutar (2020) reported that vitamin B17 (100 mg/kg b.w/day, intramuscularly, 14 days) did not significantly induce DNA damage in kidney and liver in mice in the comet assay. In another study, amygdalin (100 and 200 μM) reduced DNA damage produced by pollution in blood samples taken from C. julis collected from the port of Augusta (Syracuse, Italy), where the pollution rate is quite high.…”

“…Researchers explained that amygdalin triggered DNA repair capacity by stimulating special mechanisms (Di Mauro, Ferrito, Scifo, Renis, & Tomasello, 2017). Some researchers investigated the effects of vitamin B17 (amygdalin) in tumors developed by the administration of the Ehrlich tumor cells in mice using comet assay (El-Masry, Al-Shaalan, Tousson, Buabeid, & Al-Ghadeer., 2020;Mutar et al, 2020;Tousson et al, 2020). It was demonstrated that it significantly reduced (175 mg/Kg b.w/day, orally) the levels of tumor tissue in mice.…”

Ehrlich ascites carcinoma as model for studying the cardiac protective effects of curcumin nanoparticles against cardiac damage in female mice

“…5,6 Ehrlich ascites carcinoma (EAC) is mentioned to as a spontaneous murine mammary adenocarcinoma, is originally hyperdiploid and can transplant intraperitoneally passages on all mouse strains. 7 Chemotherapy is the most common approach used in cancer treatment, which destroys cancer cells by apoptosis induction, and it causes serious influence on the life of patients and considered to be an immediate reason for death. [8][9][10] Recently, there is an obvious increase in the usage of complementary and alternative medication.…”

Protective Role of Rockect Seed (Eruca sativa) Extract against Monosodium Glutamate-induced Hepato-renal Toxicity in Male Rats

“…There was an extremely significant increase in Alpha-fetoprotein (AFP) concentration in rats treated with accent equated to control and Tawfek et al [44] confirm that. Oxidative stress is produced by the extreme manufacture or a declined removal of free radicals in cells, the widely held of which are oxygen radicals and additional reactive oxygen species (ROS) [45].…”