Ameliorative effects of Eriobotrya japonica seed extract on cellular aging in cultured rat fibroblasts

Abstract

To investigate the effects of Eriobotrya japonica seed extract (ESE) on cellular aging, intracellular calcium homeostasis in young and senescent cells was analyzed using a rat fibroblast culture as an in vitro model system and a calcium imaging technique. The application of bradykinin (BK) transiently elicited intracellular calcium ion (Ca(2+)) increased in most of the young fibroblasts, whereas these responses were scarcely observed or were significantly attenuated in senescent cells. However, the long-term treatment of senescent cells with ESE (for 7 days) dose-dependently increased the amplitude of BK-induced responses and the percentage of BK-responding cells. In particular, most senescent cells could respond to BK with long-term treatment with ESE (1.0% or 2.0%), an effect that reinstated the percentage of BK-responding cells to the same level as that in young cells. The effects of ESE on amplitude or percentage of responding cells were not observed in young cells. Moreover, the time to half decay, which was significantly longer in senescent cells than that in young cells, was shortened in senescent cells with long-term treatment with ESE. These results suggest that treatment with an adequate concentration of ESE renders BK-induced Ca(2+) dynamics in senescent cells similar to those in young cells. Therefore, ESE can retard and/or protect against cellular aging and may be useful for elucidating the antiaging processes.

Biological Activities of Extracts from Loquat (Eriobotrya japonica Lindl.): A Review

“…Loquat extracts have shown other bioactivities such as improvement of liver function [66,67], lung [68], renal [65] and neuronal cells [46], and also loquat extracts showed anti-obesity and hypolipidemic activity [36,37,69], anti-thrombotic potential [20], antiaging effects [70], anti-allergic [22], and antinociceptive activities [24], etc.…”

“…Loquat seed extract also showed antiaging activity by ameliorating the cellular aging in cultured rat fibroblasts [70]. Treatment of loquat extract for seven days rendered bradykinin (BK)-induced Ca 2+ dynamics in senescence cells similar to those in young cells, and retarded and/or protected against cellular aging [70].…”

“…Treatment of loquat extract for seven days rendered bradykinin (BK)-induced Ca 2+ dynamics in senescence cells similar to those in young cells, and retarded and/or protected against cellular aging [70]. The antiosteoporosis effect of loquat leaf was also reported in an ovariectomized mice model [71].…”

Grape skin and loquat leaf extracts and acai puree have potent anti-atherosclerotic and anti-diabetic activity in vitro and in vivo in hypercholesterolemic zebrafish

“…In particular, LL terpenoids induce hypoglycemia in diabetic mice (3), and a hydrophilic extract of the loquat has shown antitumor activity (4). In addition to the leaf, the loquat flower and seed also possess antioxidant capacity (5), in addition to anti-senescence activities in rat fibroblasts (6).…”