Ameliorative Effects of Annona muricata (Graviola) and Fullerene C60 Against Toxicity Induced by Carboplatin in Male Albino Rats

Abstract

This study was designed to demonstrate the ameliorating effects Annona muricata (Graviola) plant extract and fullerene C 60 on hematological and biochemical disorder alteration induced by intraperitoneal (i.p.) injection of carboplatin (CRP) on male rats. Five groups of adult male rats we established (n=10). Group1: animals were administered normal saline i.p. (10 ml/kg b.wt) for 40 days and served as a normal group. Group 2: rats were injected i.p. with a single dose of CRP (80 mg/kg b.wt), then received a saline solution for the remaining 40 days and served as a control group. Group 3: animals were oral administration of Annona muricata leaves extract at a dose of 200 mg/kg/day for 40 consecutive days after 24 hours of i.p. injected with a single dose of CRP (80 mg/kg b.wt.). Group 4: animals were treated orally by fullerene C 60 (4 mg/ kg b.wt.) daily for 40 days after 24 hours of i.p. injected a single dose of CRP (80 mg/kg b.wt.). Group 5: each rat in this group was daily oral administration of Annona muricata leaves extract at a dose of (200 mg/kg b.wt.) + fullerene C 60 (4 mg/ kg b. wt.) for 40 days after 24 hours of injected a single dose of CRP. Results obtained revealed that CRP administration in the control group (gp. 2) significantly reduced the levels of red blood cells (RBCs), haemoglobin (Hb), blood Platelets (PLTs) and PCV value, with a marked reduction in white blood cells (WBCs). Additionally, there is an elevation in serum ALT, AST, ALP, total bilirubin, creatinine and urea associated with a reduction in albumin, total protein, and uric acid. In addition, hepatic and renal thiobarbituric acid reactive substances (TBARS) levels were significantly increased by CRP administration while levels of endogenous antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH) were reduced. This study confirmed the risk of increased oxidative stress, hepatotoxicity and nephrotoxicity due to CRP administration. In addition, Annona muricata leaves extract and fullerene C 60 , clarified modulatory role against the cellular damage produced by free radicalinduced by CRP.

Therapeutic Effect of Sepia pharaonis Ink Extract and Ellagic Acid on the Toxicity Induced by Cyclophosphamide in Male Albino Rats

“…High level of serum alkaline phosphatase is related to synthesis increased of it by cells lining bile canaliculi usually in response to cholestasis and increased biliary pressure (Kanchana andSadiq, 2011 andKasem, 2019). In addition, Senthilkumar et al (2006) found that hypo-proteinemia was observed in rats injected with CP for two days and attributed to the CP exerts a direct impact on plasma cells where, it inhibits protein synthesis and results in a liver damage cells.…”