Ameliorative effect of berberine against gentamicin-induced nephrotoxicity in rats via attenuation of oxidative stress, inflammation, apoptosis and mitochondrial dysfunction

Abstract

Background: Gentamicin (GM) is the commonly used antibiotics against Gram-negative infection, but the nephrotoxic potential of drug limit its clinical interest. The aim of this study was to investigate the protective effect of berberine (BER) against GM-induced nephrotoxicity and possible underlying mechanisms. Material and methods: The rats were divided into various group, namely normal, GM-control, GM þ BER (10, 20, and 40 mg/kg). Nephrotoxicity was induced by intraperitoneal administration of GM (120 mg/kg) for 7 consecutive days. BER (10, 20, and 40 mg/kg; p.o.) was also administered for the 7 days. Various biochemical, molecular, and histological parameters were assessed in serum and kidney. Results: GM-administration significantly increased (p < 0.001) the serum creatinine and blood urea nitrogen (BUN) as well as renal malonaldehyde (MDA), nitric oxide (NO) along with Kidney Injury Molecule-1 (KIM-1), Neutrophil gelatinase-associated lipocalin (NGAL), and nuclear factor-kappa B (NF-KB) renal mRNA expressions. In addition, GM also significantly decreased (p < 0.001) the renal superoxide dismutase (SOD), reduced glutathione (GSH), B-cell lymphoma 2 (Bcl-2) mRNA expression, and mitochondrial enzymes (NADH dehydrogenase and cytochrome c oxidase) activities. Rats treated with BER (20 and 40 mg/kg; p.o.) significantly and dose-dependently (p < 0.05 and p < 0.01) restore the altered levels of antioxidant, inflammatory, apoptosis, AKI markers as well as depleted mitochondrial enzymes. Histopathological abbreviations were also ameliorated by BER administration. Conclusion: Berberine exerts renoprotective effects through its anti-oxidant, anti-inflammatory, and anti-apoptotic properties.

Chrysin ameliorates ANTU‐induced pulmonary edema and pulmonary arterial hypertension via modulation of VEGF and eNOs

“…It has demonstrated that NO is an important mediator in ANTU‐induced PAH . NO is a stable free‐radical gaseous intracellular messenger existing in various isoforms, and eNOs is one of the isoforms responsible for generation of vascular endothelium NO . eNOs regulates cellular proliferation, platelet aggregation, vascular tone, and leukocyte adhesion .…”

Neuroprotective effect of Azadirachta indica standardized extract in partial sciatic nerve injury in rats: evidence from anti-inflammatory, antioxidant and anti-apoptotic studies

“…The homogenates were centrifuged at 10,000 g at 0 °C for 20 min. It was divided into aliquot to determine superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), total calcium content, nitric oxide content, membrane-bound inorganic phosphate enzyme, tumor necrosis factor-alpha (TNF-α), interleukin (IL)-1β and nuclear factor-kappaB (NF-κB) according to previously reported procedures (Adil et al, 2016[1][2]; Kandhare et al, 2016[39], 2012[48]; Visnagri et al, 2015[79]).…”

Attenuation of reserpine‑induced fibromyalgia via ROS and serotonergic pathway modulation by fisetin, a plant flavonoid polyphenol

“…Thalamus samples (50 mg) were rinsed with cold saline (0.9% sodium chloride), minced and homogenized at 1,077 x g (4˚C for 5 min) in chilled Tris buffer (10 mM, pH 7.4) and diluted up to 5 ml. The homogenates were centrifuged at 10,000 x g at 0˚C for 20 min and divided into aliquots to determine superoxide dismutase (SOD), GSH, malondialdehyde (MDA) and nitric oxide (NO) content according to previously described procedures (40)(41)(42)(43)(44).…”