Neuroprotective effect of Azadirachta indica standardized extract in partial sciatic nerve injury in rats: evidence from anti-inflammatory, antioxidant and anti-apoptotic studies

Kandhare, Amit D.; Mukherjee, Anwesha; Bodhankar, Subhash L.

doi:10.17179/excli2017-161

Cited by 16 publications

(7 citation statements)

References 76 publications

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“…Moreover, regulating neuroinflammation and inflammatory cytokines, such as TNF-α, IL-1β, and IL-6, is crucial for nerve recovery and neuroprotection in peripheral nerve injury or neuropathic pain models. [68][69][70] This finding is consistent with that observed in previous studies. In addition, owing to its lipophilicity, vanillin may be stored and released in perineural fat, which is particularly abundant around the spinal nerve roots or nerve plexuses, to exert sustained effects.…”

Section: Dovepresssupporting

confidence: 93%

Ultrasonographic Contrast and Therapeutic Effects of Hydrogen Peroxide-Responsive Nanoparticles in a Rat Model with Sciatic Neuritis

Kim,

Jo,

Lee

et al. 2024

IJN

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Purpose Peripheral nerve damage lacks an appropriate diagnosis consistent with the patient’s symptoms, despite expensive magnetic resonance imaging or electrodiagnostic assessments, which cause discomfort. Ultrasonography is valuable for diagnosing and treating nerve lesions; however, it is unsuitable for detecting small lesions. Poly(vanillin-oxalate) (PVO) nanoparticles are prepared from vanillin, a phytochemical with antioxidant and anti-inflammatory properties. Previously, PVO nanoparticles were cleaved by H 2 O 2 to release vanillin, exert therapeutic efficacy, and generate CO 2 to increase ultrasound contrast. However, the role of PVO nanoparticles in peripheral nerve lesion models is still unknown. Herein, we aimed to determine whether PVO nanoparticles can function as contrast and therapeutic agents for nerve lesions. Methods To induce sciatic neuritis, rats were administered a perineural injection of carrageenan using a nerve stimulator under ultrasonographic guidance, and PVO nanoparticles were injected perineurally to evaluate ultrasonographic contrast and therapeutic effects. Reverse transcription-quantitative PCR was performed to detect mRNA levels of pro-inflammatory cytokines, ie, tumor necrosis factor-α, interleukin-6, and cyclooxygenase-2. Results In the rat model of sciatic neuritis, PVO nanoparticles generated CO 2 bubbles to increase ultrasonographic contrast, and a single perineural injection of PVO nanoparticles suppressed the expression of tumor necrosis factor-α, interleukin-6, and cyclooxygenase-2, reduced the expression of F4/80, and increased the expression of GAP43. Conclusion The results of the current study suggest that PVO nanoparticles could be developed as ultrasonographic contrast agents and therapeutic agents for nerve lesions.

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Section: Dovepresssupporting

confidence: 93%

Ultrasonographic Contrast and Therapeutic Effects of Hydrogen Peroxide-Responsive Nanoparticles in a Rat Model with Sciatic Neuritis

Kim,

Jo,

Lee

et al. 2024

IJN

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“…During painful stimuli in burns, C fibers release substances P and neurokinin A that stimulate the release of pro-inflammatory cytokines, which causes inflammatory pain signals in response to the stress [64][65][66] . This stress response can generate pain at neuroendocrine, nociceptive, and inflammatory levels 67,68 . In addition, the stress signals can increase glucocorticoid release, reducing cellular differentiation and proliferation 69,70 .…”

Section: Discussionmentioning

confidence: 99%

Bisacurone gel ameliorated burn wounds in experimental rats via its anti-inflammatory, antioxidant, and angiogenic properties

Yan,

Li,

Gong

2023

Acta Cir. Bras.

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Purpose: To investigate putative mechanism of wound healing for chitosan-based bisacurone gel against secondary burn wounds in rats. Methods: A second-degree burn wound with an open flame using mixed fuel (2 mL, 20 seconds) was induced in Sprague Dawley rats (male, 180-220 g, n = 15, each) followed by topical treatments with either vehicle control (white petroleum gel, 1%), silver sulfadiazine (1%) or bisacurone gel (2.5, 5, or 10%) for 20 days. Wound contraction rate and paw withdrawal threshold were monitored on various days. Oxidative stress (superoxide dismutase, glutathione, malondialdehyde, and nitric oxide), pro-inflammatory cytokines (tumour necrosis factor-alpha, interleukins by enzyme-linked immunosorbent assay), growth factors (transforming growth factor-β, vascular endothelial growth factor C using real time polymerase chain reaction and Western blot assay) levels, and histology of wound skin were assessed at the end. Results: Bisacurone gel showed 98.72% drug release with a 420.90–442.70 cps viscosity. Bisacurone gel (5 and 10%) significantly ( p < 0.05) improved wound contraction rate and paw withdrawal threshold. Bisacurone gel attenuated oxidative stress, pro-inflammatory cytokines, and water content. It also enhanced angiogenesis (hydroxyproline and growth factor) and granulation in wound tissue than vehicle control. Conclusions: These findings suggested that bisacurone gel can be a potential candidate to treat burn wounds via its anti-inflammatory, antioxidant, and angiogenic properties.

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“…Riboflavin supplementation reduces hyperalgesia and inflammation ( 42 ) and inhibits histamine-dependent itch by modulating transient receptor potential vanilloid 1 ( 43 ). Pyridoxine is used for the management of sicca syndrome ( 14 ) and improves neuropathic pain, such as allodynia and hyperalgesia, via suppression of oxidative–nitrosative stress, pro-inflammatory cytokines secretion, and apoptosis ( 44 ). β-Carotene is a precursor of vitamin A, deficiency of which leads to xerophthalmia and ocular surface inflammation ( 45 , 46 ).…”

Section: Discussionmentioning

confidence: 99%

Effect of multivitamin drug on intractable dry eye symptoms

2022

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Dry eye is a disorder of tear film and ocular surface characterized by ocular discomforts. It is associated with multiple causes and sometimes intractable. We investigated the effect of oral multivitamin supplementation (MVG) on dry eyes. Tear break-up time (TBUT), fluorescein ocular surface staining score, and tear secretion Schirmer test were measured in dry eye patients refractory to conventional topical treatment. The ocular surface disease index (OSDI), visual analog pain score (VAS), and modified standardized patient evaluation of eye dryness questionnaire were analyzed. In total, 42 eyes of 42 patients were included. TBUT increased at 1 and 3 months compared to baseline (p < 0.05). OSDI decreased at 1 and 3 months compared to baseline (p < 0.05). VAS score, impact on life, and frequency of total symptoms decreased at 3 months compared to baseline (p < 0.05). Oral administration of MVG, a vitamin complex formulation, was effective in stabilizing tear stability and alleviating symptoms in patients with intractable dry eye. Thus, it may be a viable treatment option for intractable dry eye.

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Neuroprotective effect of Azadirachta indica standardized extract in partial sciatic nerve injury in rats: evidence from anti-inflammatory, antioxidant and anti-apoptotic studies

Cited by 16 publications

References 76 publications

Ultrasonographic Contrast and Therapeutic Effects of Hydrogen Peroxide-Responsive Nanoparticles in a Rat Model with Sciatic Neuritis

Ultrasonographic Contrast and Therapeutic Effects of Hydrogen Peroxide-Responsive Nanoparticles in a Rat Model with Sciatic Neuritis

Bisacurone gel ameliorated burn wounds in experimental rats via its anti-inflammatory, antioxidant, and angiogenic properties

Effect of multivitamin drug on intractable dry eye symptoms

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