Amelioration of oxidative stress by dandelion extract through CYP2E1 suppression against acute liver injury induced by carbon tetrachloride in sprague‐dawley rats

Abstract

The protective effects of common dandelion leaf water extract (DLWE) were investigated by carbon tetrachloride (CCl4) induced hepatitis in Sprague-Dawley rats. The animals were divided into five groups: normal control, DLWE control, CCl4 control, and two DLWE groups (0.5 and 2 g/kg bw). After 1 week of administering corresponding vehicle or DLWE, a single dose of CCl4 (50% CCl4/olive oil; 0.5 mL/kg bw) was administered 24 h before killing in order to produce acute liver injury. The DLWE treatment significantly decreased CCl4-induced hepatic enzyme activities (AST, ALT and LDH) in a dose dependent manner. Also, the obstructed release of TG and cholesterol into the serum was repaired by DLWE administration. Hepatic lipid peroxidation was elevated while the GSH content and antioxidative enzyme activities were reduced in the liver as a result of CCl4 administration, which were counteracted by DLWE administration. Furthermore, the hepatocytotoxic effects of CCl4 were confirmed by significantly elevated Fas and TNF-α mRNA expression levels, but DLWE down-regulated these expressions to the levels of the normal control. Highly up-regulated cytochrome P450 2E1 was also lowered significantly in the DLWE groups. These results indicate that DLWE has a protective effect against CCl4-induced hepatic damage with at least part of its effect being attributable to the attenuation of oxidative stress and inflammatory processes resulting from cytochrome P450 activation by CCl4.

Eicosapentaenoic acid protects against 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced hepatic toxicity in cultured rat hepatocytes

“…In the present study, the TCDD also elicited severe instances of liver damage (incresing LDH). LDH in serum as a biological marker for liver damage increased (Park et al 2010). Cell necrosis leads to a rise in concentration of the LDH enzyme in serum and tissue.…”

Taraxacum officinale Weber extracts inhibit LPS-induced oxidative stress and nitric oxide production via the NF-κB modulation in RAW 264.7 cells

“…In our previous study, 4 kinds of dandelion extract (hot water, water, ethanol, and methanol) were applied to LPS-induced macrophages to evaluate their antioxidative and antiinflammatory capacities through NO and malondialdehyde (MDA) production. Hot water and methanol extracts showed more potent activities than water and ethanol extracts, which indicated that luteolin and chicoric acid may be important for the amelioration of LPS-induced oxidative stress and inflammation (Park et al, 2010a). In an animal model, dandelion leaf water extract showed significant protective effects against carbon tetrachloride (CCl 4 )-induced liver injury, which indicates luteolin (including the glycosidic form) and polyphenol contents in dandelion leaf (Park et al, 2010b).…”

Karahindiba Ekstresi Yangılı ve Ağrılı Durumlarda Parasetamolün Alternatifi Olabilir mi? Biyokimyasal ve Reprodüktif Parametreler Yönünden Bir Değerlendirme

“…Parmar et al [39] determined that the decrease in GSH level of the group of PRC toxicity in comparison with the control group in rats. In other study, Park et al [58] showed that the significantly decrease (P<0.05) in GSH level of toxicity group compared to the control group and significantly increase (P<0.05) in GSH level of the water extract of Taraxacum officinale leaves compared to the toxicity group in rats. In addition, Sumanth and Rana [56] administered the alcohol extract of the Taraxacum officinale roots in two divided doses as 50 and 100 mg/kg orally,100 mg/kg dose of TOE significantly increased the level of GSH, compared to toxicity group (P<0.001), besides it was observed that any significance is not detected in 50 mg/kg dose.…”