Amelioration effect of natural bioactive flavonoids from Turbinaria ornata against oxidative stress induced neurochemical degradation and neurodegeneration

Abstract

Marine macroalgae are abundant reservoir for biologically active molecules with antioxidant, anti-inflammatory, antiapoptotic and protease inhibition activities. Neurodegeneration is a relentless neurological disorder which is characterized by progressive atrophy in the nervous system. The multifunctional properties of glycosides from macroalgae could be utilized to treat diverse pathologic aetiologies of various human diseases. The study aimed to characterize the therapeutically effective flavonol glycosides from methanolic extracts of Turbinaria ornata to prevent neurologic progressive atrophy. Subsequently the two major fractions of methanolic extracts (i.e. MWTO, fraction from 50% methanol and MTO, fraction from absolute methanol) were purified and eluted through column chromatography and confined as glycosides. MWTO fraction showed effective antioxidant, anti-proteolytic, anti-haemolytic and anticoagulant activities than MTO fraction. MWTO also exhibited promising acetylcholine and butyrylcholine esterase inhibition activities. The in-vitro study revealed that MWTO could prevent cytotoxicity and ROS formation in SH-SY5Y neuroblastoma cells and significantly enhance the cell proliferation, thus could halt the progressive atrophy of the disease in the neurons. Later, MWTO was subjected to chemical characterization by HPLC, FTIR and UHPLC-ESI/MS and predicted the aglycone molecule of flavonol glycoside as oleanolic acid. Therefore, the active therapeutic flavonol glycoside from MWTO could be used to combat neurodegeneration and related diseases.