Ameliorating Iron Overload in Intestinal Tissue of Adult Male Rats: Quercetin vs Deferoxamine

Abstract

Objective The aim of our study is to compare the role of the new natural alternative (Quercetin) with the current iron-chelation therapy (Deferoxamine (DFO)) in the effect of iron overload on small intestinal tissues and to investigate the possible underlying molecular mechanisms of such toxicity. Methods Forty-two adult male albino rats were divided into six groups: control groups, DFO, Quercetin, iron overload, iron overload+DFO, and iron overload+Quercetin groups. Animals received daily intraperitoneal injection of Deferoxamine (125 mg /kg), Quercetin (10 mg/kg), and ferric dextran (200 mg/kg) for 2 weeks. Results Iron overloaded group showed significant increase in serum iron, total iron binding capacity (TIBC), transferrin saturation percentage (TS %) hepcidin (HEPC), serum ferritin, nontransferrin bound iron (NTBI), and small intestinal tissues iron levels. Iron overload significantly increased the serum oxidative stress indicator (MDA) and reduced serum total antioxidant capacity (TAC). On the other hand, iron overload increased IL6 and reduced IL10 in small intestinal tissues reflecting inflammatory condition and increased caspase 3 reactivity indicating apoptosis and increased iNOs expressing cell indicting oxidative stress especially in ileum. In addition, it induced small intestinal tissues pathological alterations. The treatment with Quercetin showed nonsignificant differences as compared to treatment with DFO that chelated the serum and tissue iron and improved the oxidative stress and reduced tissue IL6 and increased IL10 and decreased caspase 3 and iNOs expressing cells in small intestinal tissues. Moreover, it ameliorated the iron overload induced pathological alterations. Conclusion Our study showed the potential role of Quercetin as iron chelator like DFO in case of iron overload induced small intestinal toxicity in adult rats because of its serum and tissue iron chelation, improvement of serum, and small intestinal oxidative stress, ameliorating iron induced intestinal inflammation, apoptosis, and histopathological alterations.

Analyses of hemorrhagic diathesis in high-iron diet-fed rats

“…Several studies used genetic or induced iron-overloaded animal models 12,[22][23][24][25][26] , but none showed bleeding tendency or coagulation abnormality, raising the question of what is different between the dietary model of this study and other iron-overloaded models. Of these studies, some studies using rat models have reported elevated transferrin saturation compared to that in each control; about 76% in TfR2 mutant iron-overloaded Sprague-Dawley rat 23 , 92.5% in intraperitoneally administered dextran iron-induced iron-overloaded albino rats 24 , and 95.9% in orally administered citrate iron-induced iron-overloaded Wistar rats 25 . This study showed that dietary citrate iron-induced iron-overloaded F344 rats had the same degree of transferrin saturation (95.6%).…”

The effect of the flavonol rutin on serum and liver iron content in a genetic mouse model of iron overload

“…The reduction in liver iron caused by rutin may have been insufficient to affect the expression levels of some genes, such as Tfr1, whose expression levels are usually inversely correlated with cellular iron stores. Under iron overload conditions in wild type animals, Hamp expression levels would be expected to Downloaded from http://portlandpress.com/bioscirep/article-pdf/doi/10.1042/BSR20210720/915403/bsr-2021-0720.pdf by guest on 28 June 2021 decrease upon iron chelation (38) to promote absorption and redistribution of iron stores. However, the absence of a change in Hamp in our study is likely due to dysregulated hepcidin regulation resulting from the lack of Tfr2 in these mice.…”

Intestinal Mucositis Induced by 5- Fluorouracil and the Possible Protective Effect of Probiotics Containing Lactobacillus in Adult Male Albino Rats: Histological and Immunohistochemical Study

“…After activation with HistoVT One, the immunocomplex was visualized by the avidin-biotin-peroxide method using the Vecstatin Elite ABC Rabbit IgG kit, according to the manufacturer's instructions. Sections were counter-stained with haematoxylin (16,18).…”