Background: Aspirin induced gastric mucosal injury, which leads to gastric ulcer. Adipose Derived Mesenchymal Stem Cells (ADMSC) can be used for treatment of gastric ulcer as they can differentiate into many cell types. Omeprazole is used for treatment of gastric acid-related disorders. The Aim of the Work: Was to compare between the therapeutic role of ADSMC and omeprazole in treatment of gastric ulcer Material and Methods: Forty male albino rats were divided into four equal groups: 1-Control group. 2-Aspirin group in which rats were fasted for 24 hours, then given acetylsalicylic acid (200 mg/kg) once orally for induction of ulcer. 3-ADMSCtreated group in which rats were treated by (3 × 10⁶) ADMSCs/ rat once by intravenous injection four hours after induction of ulcer. 4-Omeprazole-treated group in which rats were treated by omeprazole suspension (20 mg/kg) orally, four hours after induction of ulcer, and for five days. Gastric juice volume and acidity, length of gastric ulcer, and histopathological and immunohistochemical changes of the stomach were all evaluated. Results: Aspirin induced erosion and loss of architecture of gastric mucosal epithelium, ADMSC treatment reduced the length of gastric ulcer, ameliorate histopathological changes and reduced the PCNA-LI also the omeprazole treatment promoted healing of the gastric ulcer induced by aspirin but the therapeutic role of ADMSC was significantly lower in ulcer index and PCNA-LI and better in PAS reaction and histopathological healing of gastric ulcer than the therapeutic role of omeprazole.
Conclusion:The therapeutic role of ADMSC was found to be superior to omeprazole in treatment of gastric ulcer.
Introduction: Diclofenac (DFC) has been widely utilized as anti-inflammatory and analgesic medication. Naringin is a natural flavanone glycoside that is found in citrus fruits. Quercetin is a natural flavonoid found in vegetables and fruits. Aim: evaluation of the roles of naringin and quercetin in protection and recovery of rats' stomach ulcers caused by DFC. Material and methods: Forty male albino adult rats were divided into 4 identical groups: Control group, (DFC) group: rats were given 10 mg/kg b.w. /day DFC by oral gavage for 4 weeks, (DFC + Naringin) group: rats were given100 mg/kg/body weight naringin daily along with previous dose of DFC dose for 4 weeks and (DFC + Quercetin) group: rats were given 50 mg/kg body weight quercetin daily along with previous dose of DFC. The stomach tissues were examined grossly and processed for microscopic examination. Results: DFC group revealed ulceration of mucosa resulting in detachment of fundic mucosa, inflammatory infiltration, and wide lumen of fundic glands. There was a thin, sporadic PAS-AB mucous coating over the surface epithelium. It showed extensive iNOS immunoreaction in the cytoplasm of gastric epithelial cell and strong PCNA immunoexpression in the cells lining the fundic glands. Both naringin and quercetin exhibited a protective effect by prevention of histopathological changes caused by DFC on gastric mucosa. There is a persistent, thick mucus coating covering the surface epithelium, weak iNOS immunoreaction and moderate PCNA.
Conclusion:The intake of naringin during taking diclofenac protects the stomach mucosa but quercetin has more protection than naringin.
Background: Chlorpyrifos CPF is one of the organophosphate insecticides that were interfered with the body's hormones. Thyroid hormones are necessary to maintained normal metabolism, growth and development and the disturbance of their levels leads to adverse medical conditions. Ginger extract and selenium are effective antioxidants. Aim of the work: This study examined effect of CPF on the thyroid gland structure and function and the ameliorative effect of ginger extract and selenium. Material and Methods: forty rats were categorized into four groups. Control group I, group II: rats were given CPF at a dose of 6.7 mg/kg orally five days / week for six weeks, group III: rats were given CPF and ginger extract at a dose of 750 mg/kg orally, five days / week for six weeks. Group IV: rats were given CPF and sodium selenite 10 µg/Kg orally five days / week for six weeks, blood samples were taken for hormonal essay. Thyroid gland specimens were prepared for the histological and immunohistochemical studies. Results: chlorpyrifos induced functional toxic effect and destruction of the thyroid gland histological structures. There was a significant decrease in area % of the colloid and a significant increase in mean number of PCNA positive nuclei in comparison with the control group. Ginger extract and selenium eliminated the damage effect of CPF on the thyroid gland function and histological structure. Conclusion: CPF had adverse effects on the thyroid structure and function that could be eliminated by administration of ginger and selenium.
Introduction and aim. Hepatitis C virus (HCV) infection is a global medical problem. HLA –DRB1 alleles have an important role in immune response against HCV. The aim of this study is to clarify the contribution of HLA –DRB1 alleles in HCV susceptibility in a multicentre family-based study. Material and methods. A total of 162 Egyptian families were recruited in this study with a total of 951 individuals (255 with chronic hepatitis C (CHC), 588 persons in the control group(-ve household contact to HCV) and 108 persons who spontaneously cleared the virus (SVC). All subjects were genotyped for HLA -DRB1 alleles by SSP-PCR and sequence based typing (SBT) methods. Results. The carriage of alleles 3:01:01 and 13:01:01 were highly significant in CHC when compared
to that of control and SVC groups [OR of 3 family = 5.1289, PC (Bonferroni correction ) = 0.0002 and 5.9847, PC = 0.0001 and OR of 13 family = 4.6860, PC = 0.0002 and OR = 6.5987, PC = 0.0001 respectively]. While DRB1*040501, DRB1*040101, DRB1*7:01:01 and DRB1*110101 alleles were more frequent in SVC group than CHC patients (OR = 0.4052, PC = 0.03, OR: OR = 0.0916, PC = 0.0006, OR = 0.1833, PC = 0.0006 and OR = 0.4061, PC = 0.0001 respectively). Conclusions. It was concluded that among the Egyptian families, HLA- DRB1*030101, and DRB1*130101 alleles associated with the risk of progression to CHC infection, while DRB1*040101, DRB1*040501, DRB1*7:01:01and DRB1*110101 act as protective alleles against HCV infection.
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