Ameliorating effects of water bamboo shoot (Zizania latifolia) on acute alcoholism in a mice model and its chemical composition

Gao, Yuan; Chen, Hangjun; Liu, Ruiling; Wu, Wei; Mu, Honglei; Han, Yanchao; Yang, Hailong; Gao, Haiyan

doi:10.1016/j.foodchem.2022.132122

Cited by 18 publications

(19 citation statements)

References 34 publications

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“…The Walle-Hoch method ( 6 ) was used to determine the ADH activity of lotus root extracts in vitro . In a test tube, 0.375 mL of sodium pyrophosphate buffer (pH = 8.8), 0.25 mL of 0.027 mol/L NAD + , 0.125 mL of 12% ethanol, and 0.025 mL of different concentrations of lotus root extract was added in turn, mixed well and then incubated for 5 min at 25°C in a sealed incubator.…”

Section: Methodsmentioning

confidence: 99%

The mitigative effect of lotus root (Nelumbo nucifera Gaertn) extract on acute alcoholism through activation of alcohol catabolic enzyme, reduction of oxidative stress, and protection of liver function

Yang

Gao

et al. 2023

Front. Nutr.

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ObjectivesLotus root (Nelumbo nucifera Gaertn) is a common medicinal-food dual-use vegetable. In this study, the effects of lotus root extract on acute alcoholism were investigated.MethodsThe Walle-Hoch method was used to determine the ADH activity of lotus root extracts in vitro. Lotus root methanol extract were identified by UPLC-QTOF-MS/MS based metabolomics analysis. Then 109 active ingredients with achievable oral doses and drug-like properties were explored using the TCMSP platform. SwissTargetPrediction Database to predict lotus root treatment targets for acute alcoholismSTRING database (https://www.string-db.org/) was used to construct protein-protein interaction network graphs. Gene ontology (GO) functional, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of genes common to lotus root and alcoholism by Metascap database. Molecular docking simulations were performed using AutoDock 1.5.6 software. Animal experiments verified the relieving effect of lotus root extract on acute alcoholism after intervention.ResultsResults indicated the methanol extract of lotus root showed the highest activation rate of ethanol dehydrogenase in vitro (18.87%). The 433 compounds of lotus root methanol extract were identified by UPLC-QTOF-MS/MS based metabolomics analysis. Bioinformatics analysis indicate that there were 224 intersectioning targets between lotus root extract and acute alcoholism. KEGG enrichment analysised shows that lotus root extract may play a role in treating acute alcoholism by intervening with the neuroactive ligand-receptor interaction pathway. The protein-protein interaction network (PPI) analysis found that HSP90AA1, MAPK1 and STAT3 played a key role in lotus root extract-modulated PPI networks. Molecular docking showed that (7R, 8S)-dihydrodihydrodipine cypressol had the best binding ability with MAPK1. Experiments in mice indicate that lotus root extract improved the activity of liver alcohol dehydrogenase (ADH), acetaldehyde dehydrogenase (ALDH), catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX), increase glutathione (GSH) and reduce malondialdehyde (MDA) levels, decrease glutamate transaminase (AST), alanine transaminase (ALT) and alkaline phosphatase (AKP) in the serum of mice with acute alcoholism, and accelerate the metabolic rate of alcohol after drinking. This study reveals the mechanism of lotus root to alleviate acute alcoholism, which provides a basis for further research on functional foods using lotus root and offers new possibilities for the treatment of acute alcoholism.ConclusionsThe results of the current study showed that the methanolic extract of lotus root had the highest activation rate of ethanol dehydrogenase. Network pharmacology results suggest that lotus root extract may play a role in the treatment of alcoholism by regulating signaling pathways, such as neuroactive ligand-receptor interactions, as well as biological processes, such as regulation of secretion, regulation of ion transport, response to lipopolysaccharides, and response to alcohol. Animal experiments confirmed the therapeutic effect of lotus root on acute alcoholism mechanistically through activation of alcohol catabolic enzyme, reduction of oxidative stress and protection of liver function.

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Section: Methodsmentioning

confidence: 99%

The mitigative effect of lotus root (Nelumbo nucifera Gaertn) extract on acute alcoholism through activation of alcohol catabolic enzyme, reduction of oxidative stress, and protection of liver function

Yang

Gao

et al. 2023

Front. Nutr.

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“…Water bamboo shoot, commonly called Jiaobai or Gausun in Chinese and Makomotake in Japanese, is the spindly shoot formed from the swollen culms of Zizania latifolia when they are infected by the smut fungus Ustilago esculenta (Li et al, 2019; Zhang et al, 2012). Water bamboo shoots possess dietary polyphenols, amino acids, polysaccharide along with some minerals, which are good for human health (Gao et al, 2022; Sun et al, 2020; Zhang et al, 2012). Water bamboo shoots are widely cultivated as a delicious and nutritious aquatic vegetable in the south of the Yangtze River in China and scattered locations in Japan, Korea, and southeastern Asia countries (M. Wang et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Effect of ozone treatment on lignification and postharvest quality of water bamboo shoots

Liu

Wang²,

Yang

et al. 2022

eFood

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Ozone treatment is a useful approach to maintain postharvest quality for various fruits and vegetables. In this study, the effects of ozone fumigation on lignification and postharvest quality changes in water bamboo shoots were investigated. Water bamboo shoots were respectively treated with ozone at the concentrations of 0 (control), 3, 6, 9, and 12 µl L −1 , packaged in polyethylene packing bag and stored at 2 ± 1°C for 60 days. The results showed that ozone treatment maintained the lightness, whiteness index values and retarded the lignification during storage. Water bamboo shoots treated with 9 µl L −1 ozone showed the best fruit quality and the least lignification. Ozone treatment also slowed the decrease of soluble pectin content and cellulose accumulation by inhibiting the activities of cellulase (Cx) and polygalacturonase. In addition, ozone treatment also protected cell ultrastructure. Ozone treatment could be a potential alternative for inhibiting lignification and delaying quality deterioration of water bamboo shoots during storage.

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“…39 The polyphenols extracted from Zizania latifolia could protect mice with acute alcoholism by increasing the level of antioxidant enzymes in serum. 40 In this study, ethanol could significantly promote the depletion of GSH, SOD, and CAT in AML-12 cells, while promoting the accumulation of MDA and ROS, indicating that the antioxidant defense system of AML-12 cells was disrupted. However, dietary polyphenol pretreatment was able to significantly reduce intracellular ROS levels, with DMY being more protective than API, probably because DMY contained more hydroxyl groups in its structure than API since the number of phenolic hydroxyl groups was generally related to its antioxidant activity positive correlation.…”

Section: ■ Discussionmentioning

confidence: 52%

“…Sinapic acid could alleviate alcohol-induced oxidative stress by activating the Nrf2 signaling pathway to upregulate the expression of downstream key antioxidant proteins HO-1 and SOD . The polyphenols extracted from Zizania latifolia could protect mice with acute alcoholism by increasing the level of antioxidant enzymes in serum . In this study, ethanol could significantly promote the depletion of GSH, SOD, and CAT in AML-12 cells, while promoting the accumulation of MDA and ROS, indicating that the antioxidant defense system of AML-12 cells was disrupted.…”

Section: Discussionmentioning

confidence: 61%

Four Different Structural Dietary Polyphenols, Especially Dihydromyricetin, Possess Superior Protective Effect on Ethanol-Induced ICE-6 and AML-12 Cytotoxicity: The Role of CYP2E1 and Keap1-Nrf2 Pathways

Wang

Shang

et al. 2023

J. Agric. Food Chem.

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Polyphenols have received attention as dietary supplements for the relief of alcoholic liver disease (ALD) due to various bioactivities. Ethanol-induced rat small intestinal epithelial cell 6 (IEC-6) and alpha mouse liver 12 (AML-12) cell models were pretreated with four dietary polyphenols with different structures to explore their effects on cytotoxicity and potential protective mechanisms. The results showed that polyphenols had potential functions to inhibit ethanol-induced AML-12 and IEC-6 cell damage and oxidative stress, and restore ethanol-induced IEC-6 permeability and tight junction gene expression. Especially, dihydromyricetin (DMY) had the best protective effect on ethanol-induced cytotoxicity, followed by apigenin (API). Western blot results showed that DMY and API had the best ability to inhibit CYP2E1 and Keap1, and promote nuclear translocation of Nrf2, which might be the potential mechanism by which DMY and API attenuate ethanol-induced cytotoxicity. Moreover, the molecular docking results predicted that DMY and API could bind more tightly to the amino acid residues of CYP2E1 and Keap1, which might be one of the inhibitory modes of dietary polyphenols on CYP2E1 and Keap1. This study provided a rationale for the subsequent protective effect of dietary polyphenols on alcohol-induced liver injury in animal models and provided new clues on bioactive components for ALD-protection based on the gut-liver axis.

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Ameliorating effects of water bamboo shoot (Zizania latifolia) on acute alcoholism in a mice model and its chemical composition

Cited by 18 publications

References 34 publications

The mitigative effect of lotus root (Nelumbo nucifera Gaertn) extract on acute alcoholism through activation of alcohol catabolic enzyme, reduction of oxidative stress, and protection of liver function

The mitigative effect of lotus root (Nelumbo nucifera Gaertn) extract on acute alcoholism through activation of alcohol catabolic enzyme, reduction of oxidative stress, and protection of liver function

Effect of ozone treatment on lignification and postharvest quality of water bamboo shoots

Four Different Structural Dietary Polyphenols, Especially Dihydromyricetin, Possess Superior Protective Effect on Ethanol-Induced ICE-6 and AML-12 Cytotoxicity: The Role of CYP2E1 and Keap1-Nrf2 Pathways

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