Four Different Structural Dietary Polyphenols, Especially Dihydromyricetin, Possess Superior Protective Effect on Ethanol-Induced ICE-6 and AML-12 Cytotoxicity: The Role of CYP2E1 and Keap1-Nrf2 Pathways

Abstract

Polyphenols have received attention as dietary supplements for the relief of alcoholic liver disease (ALD) due to various bioactivities. Ethanol-induced rat small intestinal epithelial cell 6 (IEC-6) and alpha mouse liver 12 (AML-12) cell models were pretreated with four dietary polyphenols with different structures to explore their effects on cytotoxicity and potential protective mechanisms. The results showed that polyphenols had potential functions to inhibit ethanol-induced AML-12 and IEC-6 cell damage and oxidative stress, and restore ethanol-induced IEC-6 permeability and tight junction gene expression. Especially, dihydromyricetin (DMY) had the best protective effect on ethanol-induced cytotoxicity, followed by apigenin (API). Western blot results showed that DMY and API had the best ability to inhibit CYP2E1 and Keap1, and promote nuclear translocation of Nrf2, which might be the potential mechanism by which DMY and API attenuate ethanol-induced cytotoxicity. Moreover, the molecular docking results predicted that DMY and API could bind more tightly to the amino acid residues of CYP2E1 and Keap1, which might be one of the inhibitory modes of dietary polyphenols on CYP2E1 and Keap1. This study provided a rationale for the subsequent protective effect of dietary polyphenols on alcohol-induced liver injury in animal models and provided new clues on bioactive components for ALD-protection based on the gut-liver axis.

Oil body extraction from high‐fat and high‐protein soybeans by laccase cross‐linked beet pectin: physicochemical and oxidation properties

“…There are a lot of unsaturated fatty acids in soybean oil emulsion. Hydrogen peroxide and malondialdehyde were easily produced by oil oxidation 33 . With the increase in storage days, the pH had a strong influence on the peroxide value and TBARS.…”

“…Hydrogen peroxide and malondialdehyde were easily produced by oil oxidation. 33 With the increase in storage days, the pH had a strong influence on the peroxide value and TBARS. The peroxide values of emulsion at pH 6, 7 and 8 were significantly lower than those at pH 2.5 (P < 0.05).…”

Protective Effect of Polyphenols, Protein, Peptides, and Polysaccharides on Alcoholic Liver Disease: A Review of Research Status and Molecular Mechanisms

“…Alcohol is one of the most common causes of end-stage liver disease. In addition to alcoholic liver disease (ALD), more than 50% of patients with other liver diseases (including hepatitis C, nonalcoholic fatty liver disease (NAFLD) and hemochromatosis may also be associated with a large amount of alcohol intake. , Because of the dangers of excessive drinking, the alcohol consumption patterns in various regions of the world have changed. The average alcohol consumption in many European countries was decreasing, whereas the per capita alcohol consumption in Asian countries (e.g., China, India, and Vietnam) and many sub-Saharan African countries were increasing .…”

Alleviating Effect of Lacticaseibacillus rhamnosus 1.0320 Combined with Dihydromyricetin on Acute Alcohol Exposure-Induced Hepatic Impairment: Based on Short-Chain Fatty Acids and Adenosine 5′-Monophosphate-Activated Protein Kinase-Mediated Lipid Metabolism Signaling Pathway

“…Previous studies in our group have shown that pretreatment with chlorogenic acid (CHL), dihydromyricetin (DMY), apigenin (API), and phloretin (PHL) could protect ethanolinduced cytotoxicity of IEC-6 cells and AMI-12 cells. 13 Lacticaseibacillus rhamnosus (L. rhamnosus) 1.0320 has antiinflammatory, immune-enhancing, and intestinal microbiotaregulating biological activities. 14,15 Meanwhile, appropriate concentrations of CHL, DMY, API, and PHL were found to promote the growth and bacteriocin secretion of L. rhamnosus 1.0320.…”

“…Previous studies in our group have shown that pretreatment with chlorogenic acid (CHL), dihydromyricetin (DMY), apigenin (API), and phloretin (PHL) could protect ethanol-induced cytotoxicity of IEC-6 cells and AMI-12 cells Lacticaseibacillus rhamnosus (L.…”