Amebic Colitis Masquerading as Acute Inflammatory Bowel Disease

Patel, Atulkumar; DeRidder, Peter H.

doi:10.1097/00004836-198908000-00012

Cited by 24 publications

(13 citation statements)

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“…In CIE and agarose gel diffusion, antibodies may persist for 6 months or much longer (94,104,209). The gel diffusion precipitin test detected antibodies for up to 4 years following infection (150).…”

Section: Antibody Detectionmentioning

confidence: 99%

Laboratory Diagnosis of Amebiasis

2003

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The detection of Entamoeba histolytica, the causative agent of amebiasis, is an important goal of the clinical microbiology laboratory. To assess the scope of E. histolytica infection, it is necessary to utilize accurate diagnostic tools. As more is discovered about the molecular and cell biology of E. histolytica, there is great potential for further understanding the pathogenesis of amebiasis. Molecular biology-based diagnosis may become the technique of choice in the future because establishment of these protozoa in culture is still not a routine clinical laboratory process. In all cases, combination of serologic tests with detection of the parasite (by antigen detection or PCR) offers the best approach to diagnosis, while PCR techniques remain impractical in many developing country settings. The detection of amebic markers in serum in patients with amebic colitis and liver abscess appears promising but is still only a research tool. On the other hand, stool antigen detection tests offer a practical, sensitive, and specific way for the clinical laboratory to detect intestinal E. histolytica. All the current tests suffer from the fact that the antigens detected are denatured by fixation of the stool specimen, limiting testing to fresh or frozen samples

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Section: Antibody Detectionmentioning

confidence: 99%

Laboratory Diagnosis of Amebiasis

2003

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“…In these cases positive test results can indicate past infection with E. histolytica without being related to the present clinical status. Specific amebic antibodies can persist up to 4 years after infection with E. histolytica (23). In this respect the CAP test, used in this study as a reference test, is valuable for confirmation of positive test results, because after successful treatment of amebic abscess, results of this test in general become negative after 6 months to 1 year (12).…”

Section: Discussionmentioning

confidence: 87%

“…When microscopic studies of unpreserved stools are delayed for a prolonged period (Ͼ1 h), false-negative results can occur (22). As a result, patients with amebic colitis have been misdiagnosed as suffering from inflammatory bowel disease, often with dramatic consequences after initiating corticosteroid treatment (23,30). In earlier studies among patients with amebic colitis, specific antibodies were observed with a variety of tests in 70 to 90% of cases (10,17,18,24).…”

Section: Discussionmentioning

confidence: 99%

Use of Rapid Dipstick and Latex Agglutination Tests and Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Amebic Liver Abscess, Amebic Colitis, and Entamoeba histolytica Cyst Passage

Doorn¹,

Hofwegen²,

Koelewijn³

et al. 2005

J Clin Microbiol

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A homemade enzyme-linked immunosorbent assay (ELISA) and a dipstick assay (Dipstick) for the detection of anti-Entamoeba histolytica antibodies in serum were developed and evaluated together with a commercially available latex agglutination test (LAT; Laboratoires Fumouze) for their use in serodiagnosis of amebiasis. The sensitivity of these assays was evaluated with sera from 27 patients with radiologically proven, cellulose acetate precipitation (CAP) test-positive amebic liver abscess, 7 patients with parasitologically and PCR-proven amebic colitis, and 11 patients with parasitologically and PCR-proven E. histolytica cyst passage. The sensitivities of the ELISA, Dipstick, and LAT were all 93.3% (42/45). With a combination of Dipstick and LAT, all abscess and colitis patients had at least one positive result. The specificity was assessed with 238 sera from patients with various parasitic, bacterial, viral, and fungal infectious diseases, sera containing autoimmune antibodies, and sera from healthy blood donors. The specificities of the ELISA, Dipstick, and LAT were 97.1%, 98.1%, and 99.5%, respectively. Of 61 sera from patients with PCR-proven E. dispar infection, 60 (98.4%) were negative in both Dipstick and LAT and 59 (96.7%) were negative in ELISA. Our data suggest that all three assays are sensitive serological tests. The rapid LAT and Dipstick provide fast results and can easily be applied in routine laboratories in order to facilitate the diagnosis of amebiasis.

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“…Morakod Deesomsak [1] , Wattana Sukeepaisarnjaroen [2] and Kittisak Sawanyawisuth [2], [3] [1]. Samitivej Hospital, Bangkok, Thailand.…”

Section: Differential Diagnosis Of Ulcers Throughout the Colonmentioning

confidence: 99%

Differential diagnosis of ulcers throughout the colon

Deesomsak¹,

Sukeepaisarnjaroen

Sawanyawisuth

2014

Rev. Soc. Bras. Med. Trop.

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REFERENCESA 61-year-old Bristish woman, had history of visiting Far East for years, presented with abdominal cramping pain and bloody mucous diarrhea for 3 weeks. Examination of her stool showed that it contained white and red blood cells more than 100 cells/mm 3 and that her stool culture was negative for enteropathogenic organisms. A colonoscopy was performed to exclude malignancy, ulcerative colitis, or infectious colitis. The colonoscopy revealed the presence of numerous wellcircumscribed, oval shaped ulcers, size 0.2-1.0cm spanning the area from her cecum ( Figure A) to her rectum (Figure B). Tissue pathology revealed the presence of amebic trophozoites containing digested red blood cells ( Figure C). The patient was successfully treated with metronidazole. Diffuse colonic ulcers (Figures A and B) may result from several different causes, such as amebiasis, tuberculous colitis, ulcerative

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Amebic Colitis Masquerading as Acute Inflammatory Bowel Disease

Cited by 24 publications

References 0 publications

Laboratory Diagnosis of Amebiasis

Laboratory Diagnosis of Amebiasis

Use of Rapid Dipstick and Latex Agglutination Tests and Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Amebic Liver Abscess, Amebic Colitis, and Entamoeba histolytica Cyst Passage

Differential diagnosis of ulcers throughout the colon

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