Ambient pH Signalling in Yarrowia lipolytica

Blanchin‐Roland, Sylvie

doi:10.1007/978-3-642-38320-5_6

Cited by 2 publications

(2 citation statements)

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“…In C. albicans , although Rim signal dependent Rim101 C‐terminal processing occurs in both acidic and alkaline media to different extents, it is thought that the alkaline active 74 kDa form is the product of a single Rim13‐mediated proteolysis (Li et al ., ; Xu et al ., ). However, in Y. lipolytica , it has been suggested that Rim101 might be processed similarly to A. nidulans PacC (Lambert et al ., ; Blanchin‐Roland, ).…”

Section: Discussionmentioning

confidence: 99%

“…PacX is a protein of unusual domain structure, with a conspicuous coiled-coil helix amino-terminal to a typical carboxy-terminal fungal Zn cluster. The pH signalling pathway, including the central transcription factor PacC/ Rim101, is conserved throughout the ascomycetes and, to a certain extent, in the basidiomycetes and has been studied in ascomycetous yeasts and basidiomycetes (Peñalva et al, 2008;Davis, 2009;Selvig and Alspaugh, 2011;Blanchin-Roland, 2013;Cornet and Gaillardin, 2014;Obara and Kihara, 2014;Peñalva et al, 2014;Herrador et al, 2015;Ost et al, 2015, and references therein) (in addition to A. nidulans and other filamentous ascomycetes). However, the additional putative transcription factor PacX is an evolutionary novelty.…”

Section: Discussionmentioning

confidence: 99%

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Refining the pH response in Aspergillus nidulans: a modulatory triad involving PacX, a novel zinc binuclear cluster protein

Bussink

Bignell

Múnera-Huertas

et al. 2015

Molecular Microbiology

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SummaryThe A spergillus nidulans PacC transcription factor mediates gene regulation in response to alkaline ambient pH which, signalled by the Pal pathway, results in the processing of PacC72 to PacC27 via PacC53. Here we investigate two levels at which the pH regulatory system is transcriptionally moderated by pH and identify and characterise a new component of the pH regulatory machinery, PacX. Transcript level analysis and overexpression studies demonstrate that repression of acid‐expressed pal F, specifying the Pal pathway arrestin, probably by PacC27 and/or PacC53, prevents an escalating alkaline pH response. Transcript analyses using a reporter and constitutively expressed pac C trans‐alleles show that pac C preferential alkaline‐expression results from derepression by depletion of the acid‐prevalent PacC72 form. We additionally show that pac C repression requires PacX. pac X mutations suppress PacC processing recalcitrant mutations, in part, through derepressed PacC levels resulting in traces of PacC27 formed by pH‐independent proteolysis. pac X was cloned by impala transposon mutagenesis. PacX, with homologues within the Leotiomyceta, has an unusual structure with an amino‐terminal coiled‐coil and a carboxy‐terminal zinc binuclear cluster. pacX mutations indicate the importance of these regions. One mutation, an unprecedented finding in A . nidulans genetics, resulted from an insertion of an endogenous Fot1‐like transposon.

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Section: Discussionmentioning

confidence: 99%