Plasma membrane H+ pump at a crossroads of acidic and iron stresses in yeast-to-hypha transition

Cogo, Antônio Jesus Dorighetto; Façanha, Arnoldo Rocha; Teixeira, Layz Ribeiro da Silva; Souza, Sávio Bastos de; Rocha, Janiélio Gonçalves da; Figueira, Frederico Firme; Eutrópio, Frederico Jacob; Bertolazi, Amanda Azevedo; Rezende, Carlos Eduardo de; Krohling, César Abel; Okorokov, Lev A.; Cruz, Cristina; Ramos, Alessandro Coutinho; Okorokova‐Façanha, Anna L.

doi:10.1039/d0mt00179a

Cited by 4 publications

(3 citation statements)

References 65 publications

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“…To counteract this, the cell increased synthesis of amino acids (Aat2p/B02178g, Leu1p/B01364g, Gcv2p, Sam2p, Met6p/E12683g, Ilv5p/D03135g, Ses1p/F02629g, Gat1p/F17886g, and Shm1p/D22484g), molecular chaperones (Kar2p/E13706g, Sse1p/E13255g, and Ssa4p/D22352g), superoxide dismutase (Sod2p/B08921g), and the target aKGA, which are all known to play a role in scavenging ROS. The proposed mechanism is consistent with findings by Cogo et al ( 2020 ), showing that acidification of external pH is accompanied by enhanced H + efflux through the operation of plasma membrane H + -ATPase Pma1p (B22066g). Transportation of carboxylic acids exploits this preformed membrane H + gradient.…”

Section: External Stress Factorssupporting

confidence: 91%

“…It is known that even under extreme acidic environmental conditions (pH 3.0), the intracellular pH of a eukaryotic cell can still be maintained near neutrality (pH ~ 6.8), but the metabolic effort required to maintain the homeostasis is significant (Madshus 1988 ). It was shown that under pH 3.0, the abundance and activity of plasma membrane H + -ATPase Pma1p (B22066g) is significantly higher than at pH 4.5 or 6.0 (when it pumps H + ions inwardly) (Cogo et al 2020 ). Pma1 which generates the proton gradient and drives the active transport of nutrients by H + -symport is the most abundant protein in the yeast plasma membrane (Ferreira et al 2001 ).…”

Section: External Stress Factorsmentioning

confidence: 99%

“…The interplay between acidic pH (3.0, 4.5 vs. 6.0) and supplementation with iron ions (1 mM and 2 mM FeSO 4 ) on stress response and H + fluxes in Y. lipolytica was addressed by Cogo et al ( 2020 ). Upon combination of the two stress factors, it was clear that a pH of 3.0 had any (negative) impact on the Y. lipolytica growth rate.…”

Section: External Stress Factorsmentioning

confidence: 99%

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“Fight-flight-or-freeze” – how Yarrowia lipolytica responds to stress at molecular level?

Celińska

2022

Appl Microbiol Biotechnol

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Yarrowia lipolytica is a popular yeast species employed in multiple biotechnological production processes. High resistance to extreme environmental conditions or metabolic burden triggered by synthetically forced over-synthesis of a target metabolite has its practical consequences. The proud status of an “industrial workhorse” that Y. lipolytica has gained is directly related to such a quality of this species. With the increasing amount of knowledge coming from detailed functional studies and comprehensive omics analyses, it is now possible to start painting the landscape of the molecular background behind stress response and adaptation in Y. lipolytica. This review summarizes the current state-of-art of a global effort in revealing how Y. lipolytica responds to both environmental threats and the intrinsic burden caused by the overproduction of recombinant secretory proteins at the molecular level. Detailed lists of genes, proteins, molecules, and biological processes deregulated upon exposure to external stress factors or affected by over-synthesis of heterologous proteins are provided. Specificities and universalities of Y. lipolytica cellular response to different extrinsic and intrinsic threats are highlighted. Key points • Y. lipolytica as an industrial workhorse is subjected to multiple stress factors. • Cellular responses together with involved genes, proteins, and molecules are reviewed. • Native stress response mechanisms are studied and inspire engineering strategies.

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Section: External Stress Factorssupporting

confidence: 91%

Section: External Stress Factorsmentioning

confidence: 99%

Section: External Stress Factorsmentioning

confidence: 99%

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“Fight-flight-or-freeze” – how Yarrowia lipolytica responds to stress at molecular level?

Celińska

2022

Appl Microbiol Biotechnol

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Transcription factors enhancing synthesis of recombinant proteins and resistance to stress in Yarrowia lipolytica

Gorczyca

Nicaud

Celińska

2023

Appl Microbiol Biotechnol

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Resistance to environmental stress and synthesis of recombinant proteins (r-Prots) are both complex, strongly interconnected biological traits relying on orchestrated contribution of multiple genes. This, in turn, makes their engineering a challenging task. One of the possible strategies is to modify the operation of transcription factors (TFs) associated with these complex traits. The aim of this study was to examine the potential implications of selected five TFs (HSF1-YALI0E13948g, GZF1-YALI0D20482g, CRF1-YALI0B08206g, SKN7-YALI0D14520g, and YAP-like-YALI0D07744g) in stress resistance and/or r-Prot synthesis in Yarrowia lipolytica. The selected TFs were over-expressed or deleted (OE/KO) in a host strain synthesizing a reporter r-Prot. The strains were subjected to phenotype screening under different environmental conditions (pH, oxygen availability, temperature, and osmolality), and the obtained data processing was assisted by mathematical modeling. The results demonstrated that growth and the r-Prot yields under specific conditions can be significantly increased or decreased due to the TFs’ engineering. Environmental factors “awakening” individual TFs were indicated, and their contribution was mathematically described. For example, OE of Yap-like TF was proven to alleviate growth retardation under high pH, while Gzf1 and Hsf1 were shown to serve as universal enhancers of r-Prot production in Y. lipolytica. On the other hand, KO of SKN7 and HSF1 disabled growth under hyperosmotic stress. This research demonstrates the usefulness of the TFs engineering approach in the manipulation of complex traits and evidences newly identified functions of the studied TFs. Key points • Function and implication in complex traits of 5 TFs in Y. lipolytica were studied. • Gzf1 and Hsf1 are the universal r-Prots synthesis enhancers in Y. lipolytica. • Yap-like TF’s activity is pH-dependent; Skn7 and Hsf1 act in osmostress response.

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Pichia kudriavzevii UNJCC Y-137 and Candida tropicalis UNJCC Y-140 isolated from Durio kutejensis as potential probiotic agents

Sukmawati,

Adisyahputra,

Al-Ani

et al. 2024

Food Sci Biotechnol

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Plasma membrane H+ pump at a crossroads of acidic and iron stresses in yeast-to-hypha transition

Abstract: Iron is an essential nutrient but is toxic in excess mainly under acidic conditions. Yeasts have emerged as low cost, highly efficient soil inoculants for decontamination of metal-polluted areas, harnessing...

Cited by 4 publications

References 65 publications

“Fight-flight-or-freeze” – how Yarrowia lipolytica responds to stress at molecular level?

“Fight-flight-or-freeze” – how Yarrowia lipolytica responds to stress at molecular level?

Transcription factors enhancing synthesis of recombinant proteins and resistance to stress in Yarrowia lipolytica

Pichia kudriavzevii UNJCC Y-137 and Candida tropicalis UNJCC Y-140 isolated from Durio kutejensis as potential probiotic agents

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