Amazoninema gen. nov., (Synechococcales, Pseudanabaenaceae) a novel cyanobacteria genus from Brazilian Amazonian rivers

Abstract

The genus Leptolyngbya includes morphotypes with thin cells and simple morphology, and is one of the most common cyanobacterial genera found in a wide range of environments. In many cases, however, the morphotypes assigned to this genus do not share a common ancestor based on 16S rRNA gene phylogeny, which has led to the description of novel genera, such as Nodosilinea, Oculatella, Pantanalinema, Alkalinema, Thermoleptolyngbya, Onodrimia, Timaviella and Toxifilum. Thus, four novel isolates, with a comparable morphology to Leptolyngbya, were recovered from the Amazon and Solimões rivers. The novel 16S rRNA gene sequences obtained from these strains were placed together as a new and distinct phylogenetic lineage that is more closely related to the clusters embracing the genera Nodosilinea, Haloleptolyngbya and Halomicronema than to the genus Leptolyngbya. Additionally, these novel 16S rRNA gene sequences showed similarity values lower than 95 % compared with those from the most phylogenetic related groups and/or established genera. Altogether, these results supported the erection of a novel genus, named Amazoninema, to accommodate the novel isolates. Likewise, a comparison of their 16S rRNA gene sequences revealed similarities higher than 99.8 %, indicating that they belong to a single species, which was corroborated by analysing their 16S-23S internal transcribed spacer regions and unique Box-B helix pattern. Few studies have been undertaken to uncover the cultured diversity of cyanobacteria from Amazonia, and to our knowledge, this is the first cyanobacteria genus erected, considering morphotypes isolated exclusively from Brazilian Amazonian rivers.

Influence of Abiotic Factors on the Growth of Cyanobacteria Isolated from Nakdong River, South Korea1

“…Morphological and phylogenetic characteristics of the isolate Amazoninema brasiliense DS_2B_I2 are in agreement with the description of Amazoninema brasiliense CMAA 1609 (MF002133; Genuario et al. 2018). Amazoninema is a new genus with one species of A. brasiliense that was isolated from the Solimões River, Amazonia, Brazil (Genuario et al.…”

“…Amazoninema is a new genus with one species of A. brasiliense that was isolated from the Solimões River, Amazonia, Brazil (Genuario et al. 2018). The isolate Microcystis elabens GJ_2B_I3 had similarity to M. elabens NIES‐ 42, which was first isolated from Japan by Kondo et al.…”

Dereplication of Natural Products with Antimicrobial and Anticancer Activity from Brazilian Cyanobacteria

“…Brazilian biomes are characterized by unusually high biodiversity, and new cyanobacterial taxa are frequently described from these biomes [34][35][36][37][38][39]. Strains isolated from these biomes have antimicrobial and anticancer activities [40][41][42].…”

Monilinema gen. nov., a homocytous genus (Cyanobacteria, Leptolyngbyaceae) from saline–alkaline lakes of Pantanal wetlands, Brazil

“…2015, Genuário et al. 2018). Although polyphasic evaluation is sometimes difficult, this strategy has been recommended to reach a more consistent and natural classification of cyanobacteria group (Komarek 2006).…”