AM–FM techniques in the analysis of optical coherence tomography signals

Pitris, Costas; Kartakoullis, Andreas; Bousi, Evgenia

doi:10.1002/jbio.200910023

Cited by 5 publications

(2 citation statements)

References 18 publications

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“…Significantly, these stress granules can appear and disappear without the initiation of apoptosis and therefore act as a sensitive marker of cellular health [41]. Areas with 1 µm differences in average scatter diameter can be identified/classified using an amplitude and frequency (AM-FM) modulation analysis of the OCT signal to detect subtle changes in the spectral profile resulting from variations in the size of the scatters [42]. Pitris et al used polystyrene beads dispersed in polyacrylamide gels as imaging phantoms to demonstrate a high degree of accuracy [43] in the discrimination of the scatterer particle size.…”

Section: Discussionmentioning

confidence: 99%

Non-Invasive Detection of Early Retinal Neuronal Degeneration by Ultrahigh Resolution Optical Coherence Tomography

et al. 2014

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Optical coherence tomography (OCT) has revolutionises the diagnosis of retinal disease based on the detection of microscopic rather than subcellular changes in retinal anatomy. However, currently the technique is limited to the detection of microscopic rather than subcellular changes in retinal anatomy. However, coherence based imaging is extremely sensitive to both changes in optical contrast and cellular events at the micrometer scale, and can generate subtle changes in the spectral content of the OCT image. Here we test the hypothesis that OCT image speckle (image texture) contains information regarding otherwise unresolvable features such as organelle changes arising in the early stages of neuronal degeneration. Using ultrahigh resolution (UHR) OCT imaging at 800 nm (spectral width 140 nm) we developed a robust method of OCT image analyses, based on spatial wavelet and texture-based parameterisation of the image speckle pattern. For the first time we show that this approach allows the non-invasive detection and quantification of early apoptotic changes in neurons within 30 min of neuronal trauma sufficient to result in apoptosis. We show a positive correlation between immunofluorescent labelling of mitochondria (a potential source of changes in cellular optical contrast) with changes in the texture of the OCT images of cultured neurons. Moreover, similar changes in optical contrast were also seen in the retinal ganglion cell- inner plexiform layer in retinal explants following optic nerve transection. The optical clarity of the explants was maintained throughout in the absence of histologically detectable change. Our data suggest that UHR OCT can be used for the non-invasive quantitative assessment of neuronal health, with a particular application to the assessment of early retinal disease.

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Section: Discussionmentioning

confidence: 99%

Non-Invasive Detection of Early Retinal Neuronal Degeneration by Ultrahigh Resolution Optical Coherence Tomography

et al. 2014

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“…It will focus on OCT technology development including light source and delivery probe technology, OCT signal processing as well as multi-modal and functional extensions of OCT. After a critical review about the commercial state-of-the-art of OCT [3], recent developments of endoscopic deliver probe [4], Fourier Mode Locked Laser [5] as well as OCT signal post processing [6] technology is presented. Three-and four-dimensional OCT in the field of tissue engineering and cell signalling [7] is followed by three papers presenting multimodal imaging in the field of developmental biology using OCT and fluorescence imaging [8], in dermatology comparing OCT with multiphoton tomography [9] and in the field of ophthalmology performing in vitro OCT and Raman spectroscopy [10].…”

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confidence: 99%