Alveolar macrophages from sheep naturally infected by visna-maedi virus contribute to IL-8 production in the lung

Legastelois, Isabelle; Cottin, Vincent; Mornex, Jean‐François; Cordier, G

doi:10.1016/s0165-2427(97)00055-x

Cited by 12 publications

(12 citation statements)

References 16 publications

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“…Up-regulation of AM GM-CSF mRNA expression was observed 7 days after endoscopic instillation of VMV. This finding is consistent with those of previous studies of naturally infected sheep lungs, which demonstrated up-regulation of AM GM-CSF expression in combination with up-regulation of other proinflammatory cytokines and/or observable lung inflammation (29,50). AM IL-8 mRNA expression also was increased in virus-treated lung lobes compared to that of untreated lung lobes but not compared to that of mock-treated lobes, suggesting that the IL-8 response either was due to the BAL procedure or was induced by components of the tissue culture medium of virus and mock virus instillates.…”

Section: Discussionsupporting

confidence: 93%

“…AMs also have been implicated as the main cell type involved in VMV-induced lung inflammation, primarily by the dysregulation of cytokine gene expression and subsequent recruitment of inflammatory cells (29,50). It is possible that AMs mediate a similar inflammatory response during early VMV infection in vivo, as suggested by the observed up-regulation of interleukin-8 (IL-8) expression by AMs within 48 h of in vitro infection (28).…”

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confidence: 99%

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Role of Alveolar Macrophages in Respiratory Transmission of Visna/Maedi Virus

McNeilly

Baker

Brown

et al. 2008

J Virol

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A major route of transmission of Visna/maedi virus (VMV), an ovine lentivirus, is thought to be via the respiratory tract, by inhalation of either cell-free or cell-associated virus. In previous studies, we have shown that infection via the lower respiratory tract is much more efficient than via upper respiratory tissues (T. N. In this study, we use an experimental in vivo infection model that allowed the infection of specific segments of the ovine lung. We demonstrate that resident AMs are capable of VMV uptake in vivo and that this infection is associated with a specific up-regulation of AM granulocyte-macrophage colony-stimulating factor mRNA expression (P < 0.05) and an increase in bronchoalveolar lymphocyte numbers (P < 0.05), but not a generalized inflammatory response 7 days postinfection. We also demonstrate that both autologous and heterologous VMV-infected AMs are capable of transmitting virus after lower, but not upper, respiratory tract instillation and that this transfer of virus appears not to involve the direct migration of virus-infected AMs from the airspace. These results suggest that virus is transferred from AMs into the body via an intermediate route. The results also suggest that the inhalation of infected AMs represents an additional mechanism of virus transmission.Visna/maedi virus (VMV) is a member of the lentivirus subgroup of retroviruses that cause lymphoid interstitial pneumonia, encephalitis, arthritis, and mastitis in sheep (40,41). The main routes of transmission of VMV are thought to be through the ingestion of infected colostrum and/or milk or through the inhalation of infected respiratory secretions (6, 34). We recently have demonstrated that a major route of respiratory transmission is likely via the uptake of cell-free VMV in the lower respiratory tract (33), although the initial target cells for cell-free VMV at this site were not identified.

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Section: Discussionsupporting

confidence: 93%

mentioning

confidence: 99%

Role of Alveolar Macrophages in Respiratory Transmission of Visna/Maedi Virus

McNeilly

Baker

Brown

et al. 2008

J Virol

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“…The spontaneous release of IL-8 may be a reaction by primary cells to the foreign environment of tissue culture. Indeed, it has been reported that pulmonary ovine macrophages do not spontaneously produce IL-8 in situ in normal sheep lungs [29]. It is possible that the in vitro environment lacks the down-regulatory elements (such as IL-10) that can suppress IL-8 production.…”

Section: Identification Of the Early Release Of Cytokines By Cells Inmentioning

confidence: 99%

Cytokine release by ovine macrophages following infection withChlamydia psittaci

Entrican

Wilkie

McWaters

et al. 1999

Clinical and Experimental Immunology

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Chlamydia psittaci is an obligate intracellular pathogen that causes abortion in both sheep and humans. The disease in sheep (but not humans) is characterized by a long-term persistent phase that appears to be under the control of interferon-gamma. However, nothing is known about cytokine induction that precedes the persistent phase in sheep. Primary alveolar lavage cells recovered from normal adult sheep were used to study cytokine production in the first 72 h of infection with C. psittaci. These cells were phenotypically characteristic of macrophages, being adherent, phagocytic, CD14+ and staining positive for non-specific esterase. In vitro infection of the macrophages with C. psittaci resulted in the release of IL-1beta, IL-8 and granulocyte-macrophage colony-stimulating factor (GM-CSF) as measured by ovine-specific ELISAs. Heat-treated chlamydiae (1 h at 65 degrees C) did not induce the release of IL-1beta, but the release of IL-8 was similar to that induced by untreated organisms. The cells from different sheep varied most notably in their patterns of GM-CSF release in response to heat-treated and untreated organisms.

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“…As células alvo são os monócitos e macrófagos e a expressão viral está associada à maturação delas (Gendelman et al, 1986). Há infiltração de leucócitos mononucleares nos órgãos e tecidos alvo atingidos (Joag et al, 1996) e a secreção de citocinas pode estar envolvida na patogenia (Lechner et al, 1997;Legastelois et al, 1997).…”

Section: Introductionunclassified

Detecção da infecção pelo vírus da artrite-encefalite caprina: imunodifusão em ágar e reação em cadeia da polimerase com "primers" degenerados

Rutkoski¹,

Werenicz

Reischak³

et al. 2001

Arq. Bras. Med. Vet. Zootec.

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O objetivo deste trabalho foi analisar amostras de soro e de células sangüíneas de caprinos para detecção de anticorpos e DNA proviral do vírus da artrite-encefalite caprina (CAEV), respectivamente. Utilizou-se a técnica de imunodifusão em ágar (AGID) e a reação em cadeia da polimerase (PCR) com "primers" degenerados. Foram analisadas amostras de diferentes procedências: 39 de Mato Grosso do Sul (MS), 19 de São Paulo (SP) e 22 do Ceará (CE), dessas últimas, 12 oriundas de animais importados do Canadá. Os resultados de AGID e PCR foram discordantes, pois o primeiro permitiu a detecção de 25 animais soropositivos, enquanto a PCR detectou DNA proviral de CAEV em 16 amostras. Pela PCR foi possível identificar animais infectados cujos testes sorológicos foram negativos pelo AGID: oito amostras do MS e um do CE. São discutidos diferentes aspectos que poderiam estar envolvidos na discordância dos resultados.

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Alveolar macrophages from sheep naturally infected by visna-maedi virus contribute to IL-8 production in the lung

Cited by 12 publications

References 16 publications

Role of Alveolar Macrophages in Respiratory Transmission of Visna/Maedi Virus

Role of Alveolar Macrophages in Respiratory Transmission of Visna/Maedi Virus

Cytokine release by ovine macrophages following infection withChlamydia psittaci

Detecção da infecção pelo vírus da artrite-encefalite caprina: imunodifusão em ágar e reação em cadeia da polimerase com "primers" degenerados

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