Alternatively spliced transcripts of Sparus aurata insulin-like growth factor 1 are differentially expressed in adult tissues and during early development

Tiago, Daniel M.; Laizé, Vincent; Cancela, M. Leonor

doi:10.1016/j.ygcen.2008.04.006

Cited by 32 publications

(29 citation statements)

References 34 publications

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“…Gilthead sea bream GH and IGF-I were previously cloned (Martínez-Barberá et al 1994;Tiago et al 2008). Also, expression analyses assessing the influence of different experimental conditions, including osmoregulatory approaches, were performed (Laiz-Carrión et al 2009;Vargas-Chacoff et al 2009a, b;Fuentes et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Acclimation to different environmental salinities induces molecular endocrine changes in the GH/IGF-I axis of juvenile gilthead sea bream (Sparus aurata L.)

2014

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To assess the role of the GH/IGF-I axis in osmotic acclimation of the gilthead seabream Sparus aurata, juvenile specimens were acclimated to four environmental salinities: hyposmotic (5 ‰), isosmotic (12 ‰) and hyperosmotic (40 and 55 ‰). The full-length cDNAs for both pituitary adenylate cyclase-activating peptide (PACAP) and prepro-somatostatin-I (PSS-I), the precursor for mature somatostatin-I (SS-I), were cloned. Hypothalamic PACAP and PSS-I, hypophyseal growth hormone (GH) and prolactin (PRL), and hepatic insulin-like growth factor-I (IGF-I) mRNA expression levels were analyzed in the four rearing salinities tested. PACAP and IGF-I mRNA values increased significantly in response to both 5 and 55 ‰ salinities, showing a U-shaped curve relationship with the basal level in the 40 ‰ group. Hypothalamic PSS-I expression increased strongly in the 55 ‰ environment. GH mRNA levels did not change in any of the tested environmental salinities. PRL mRNA maximum levels were encountered in the 5 and 12 ‰ environments, but significantly down-regulated in the 40 ‰. Plasma cortisol levels significantly increased in the 40 ‰ environment. These results are discussed in relation to the well-known high adaptability of Sparus aurata to different environmental salinities and the role of the GH/IGF-I axis in this process.

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Section: Discussionmentioning

confidence: 99%

Acclimation to different environmental salinities induces molecular endocrine changes in the GH/IGF-I axis of juvenile gilthead sea bream (Sparus aurata L.)

2014

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“…Eggs, larvae, juvenile and adult fish Gilthead seabream eggs were collected from natural spawning and further cultured as previously described by Tiago and coworkers [26]. Eggs, embryos, larvae, juvenile and adult fish were sampled for RNA preparation, in situ hybridization and histology.…”

Section: Methodsmentioning

confidence: 99%

Four-and-a-half LIM domains protein 2 (FHL2) is associated with the development of craniofacial musculature in the teleost fish Sparus aurata

Rafael

Laizé

Bensimon-Brito

et al. 2011

Cell. Mol. Life Sci.

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Four-and-a-half LIM domains protein 2 (FHL2) is involved in major cellular mechanisms such as regulation of gene transcription and cytoskeleton modulation, participating in physiological control of cardiogenesis and osteogenesis. Knowledge on underlying mechanisms is, however, limited. We present here new data on FHL2 protein and its role during vertebrate development using a marine teleost fish, the gilthead seabream (Sparus aurata L.). In silico comparison of vertebrate protein sequences and prediction of LIM domain three-dimensional structure revealed a high degree of conservation, suggesting a conserved function throughout evolution. Determination of sites and levels of FHL2 gene expression in seabream indicated a central role for FHL2 in the development of heart and craniofacial musculature, and a potential role in tissue calcification. Our data confirmed the key role of FHL2 protein during vertebrate development and gave new insights into its particular involvement in craniofacial muscle development and specificity for slow fibers.

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“…In Atlantic salmon, increased igf1 expression marks the transition from zero to fast growth and is followed by the increased expression of myosin genes including mlc2 (Bower et al 2008). Three alternatively spliced transcripts of igf1 have been isolated in the gilthead sea bream (Tiago et al 2008) and each has a distinct expression pattern during development. The primers used in the present study were designed to amplify all three igf1 transcripts collectively.…”

Section: Gene Expression and Muscle Developmentmentioning

confidence: 99%

“…However, whereas these authors reported that igf1 expression increases significantly between 29 and 45 dph and between 52 and 60 dph, our findings demonstrate that igf1 increases significantly only between 25 and 35 dph and then decreases to the level observed at the beginning of the study. A third pattern of developmental regulation was founded by Tiago et al (2008), with expression of the two main transcripts of igf1 in gilthead sea bream larvae (igf1c and igf1a) decreasing significantly from 48 to 67 dph. Further studies are needed to elucidate the cause of the discrepancies between the studies.…”

Section: Gene Expression and Muscle Developmentmentioning

confidence: 99%

Transient up- and down-regulation of expression of myosin light chain 2 and myostatin mRNA mark the changes from stratified hyperplasia to muscle fiber hypertrophy in larvae of gilthead sea bream (Sparus aurata L.)

et al. 2015

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Hyperplasia and hypertrophy are the two mechanisms by which muscle develops and grows. We study these two mechanisms, during the early development of white muscle in Sparus aurata, by means of histology and the expression of structural and regulatory genes. A clear stage of stratified hyperplasia was identified early in the development of gilthead sea bream but ceased by 35 dph when hypertrophy took over. Mosaic recruitment of new white fibers began as soon as 60 dph. The genes mlc2a and mlc2b were expressed at various levels during the main phases of hyperplasia and hypertrophy. The genes myog and mlc2a were significantly upregulated during the intensive stratified formation of new fibers and their expression was significantly correlated. Expression of mstn1 and igf1 increased at 35 dph, appeared to regulate the hyperplasia-to-hypertrophy transition, and may have stimulated the expression of mlc2a, mlc2b and col1a1 at the onset of mosaic hyperplasia. The up-regulation of mstn1 at transitional phases in muscle development indicates a dual regulatory role of myostatin in fish larval muscle growth.

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Alternatively spliced transcripts of Sparus aurata insulin-like growth factor 1 are differentially expressed in adult tissues and during early development

Cited by 32 publications

References 34 publications

Acclimation to different environmental salinities induces molecular endocrine changes in the GH/IGF-I axis of juvenile gilthead sea bream (Sparus aurata L.)

Acclimation to different environmental salinities induces molecular endocrine changes in the GH/IGF-I axis of juvenile gilthead sea bream (Sparus aurata L.)

Four-and-a-half LIM domains protein 2 (FHL2) is associated with the development of craniofacial musculature in the teleost fish Sparus aurata

Transient up- and down-regulation of expression of myosin light chain 2 and myostatin mRNA mark the changes from stratified hyperplasia to muscle fiber hypertrophy in larvae of gilthead sea bream (Sparus aurata L.)

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