Alternative weed hosts harbors 16SrII group phytoplasmas associated with little leaf and witches’ broom diseases of various crops in India

Thorat, Vipool; Bhale, U. N.; Sawant, V. S.; More, Vijay Shankarrao; Jadhav, P. N.; Mane, Shamsundar Shioram; Nandanwar, R. S.; Tripathi, Savarni; Yadav, Amit

doi:10.5958/2249-4677.2016.00009.8

Cited by 22 publications

(4 citation statements)

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“…5 B) show that cowpea is a new host of 16SrII-D subgroup phytoplasmas in Egypt. Several phytoplasma groups and subgroups, including 16SrV and 16SrXII-B, were identified in cowpea from Australia (De La Rue et al, 2001;Saqib et al, 2006) and 16SrI-B, 16SrII-D, and 16SrIX from India (Kumar et al, 2012;Thorat et al, 2016;Rao et al, 2018). In addition, sequencing analysis results of the present study indicated that cauliflower is a new host of the 16SrII-A subgroup in Egypt.…”

Section: Table (1): the Incidence Of Phytoplasma On Different Crops D...supporting

confidence: 56%

Prevalence and genetic diversity of phytoplasmas infecting different crops in Egypt

Ahmed,

Kheder,

Hamouda

et al. 2024

Egyptian Journal of Phytopathology

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Phytoplasma-related symptoms such as big bud, witches' broom, phyllody, virescence, flower proliferation, little leaves, yellowing, and stem fasciation were observed in 9 plant species during a field survey in Fayoum Governorate, Egypt, in 2021 and 2022. Disease incidence on various crops ranged from 1 to 21.3% in 2021, with the percentage rising in 2022. In 2022, the highest incidence percentage (23 % (was recorded on sesame while on cowpea the lowest percentage of incidence was recorded being (1.2%). A nested PCR assay employing the universal primer pair P1/P7 followed by R16F2n/R16R2 was used to detect the presence of phytoplasmas by amplifying a segment of the phytoplasma 16S rRNA gene. The resulting amplicons were purified and sequenced. The sequences were deposited in GenBank. Sequence comparison, phylogenetic study, and virtual RFLP analysis of 16S rRNA indicated the presence of diverse phytoplasma ribosomal groups and subgroups infecting different crops ( 16SrVI-A, 16SrIII-A, 16SrII-D, 16SrII-A, 16SrI-A, and 16SrI-B). The 16SrVI-A phytoplasmas were detected in symptomatic tomato plants, the 16SrII-D phytoplasmas subgroup was identified in sesame, faba bean, and cowpea exhibiting phyllody symptoms, the 16SrII-A subgroup was detected in cauliflower. The 16SrIII-A and 16SrI-A phytoplasmas subgroups were identified in alfalfa witches' broom and chamomile, respectively for the first time in Egypt. Moreover, subgroup 16SrI-B was detected for the first time in Egypt in chrysanthemum and cabbage. This study showed the genetic diversity of phytoplasma strains infecting a variety of crops in Fayoum Governorate.

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Section: Table (1): the Incidence Of Phytoplasma On Different Crops D...supporting

confidence: 56%

Prevalence and genetic diversity of phytoplasmas infecting different crops in Egypt

Ahmed,

Kheder,

Hamouda

et al. 2024

Egyptian Journal of Phytopathology

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“…1a & b) with 4% incidence. The symptoms like bud proliferation on the main shoot and stunting (Kumar et al 2012) and little leaf, witches' broom, leaf yellowing and stem fasciations (Thorat et al 2016;Rao et al 2017b, Al-Kuwaiti et al 2019 have been reported to be caused by phytoplasma in cowpea. Similarly, symptoms of little leaf and thickened leaves, phyllody, the proliferation of shoot, wrinkled and malformed leaves, stem fasciations and stunting associated with phytoplasma infection in cowpea have also been reported from Iran (Kardani and Jamshidi 2018).…”

Section: Resultsmentioning

confidence: 99%

“…Association of phytoplasma caused by Candidatus Phytoplasma asteris, a 16SrI-B subgroup in cowpea showing bud proliferation on the main shoot and stunting was rst reported from India by Kumar et al (2012). Subsequently, Thorat et al (2016) and Rao et al (2017b) reported the association of respectively, Ca. P. aurantifolia, 16SrII-D subgruop and Ca.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a novel strain of Candidatus Phytoplasma aurantifolia infecting cowpea (Vigna unguiculata) based on 16S rDNA sequence analysis

Akhtar

Tripathi

Akram

et al. 2022

Preprint

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An attempt was made to characterize the Phytoplasma in suspected cowpea (Vigna unguiculata L.) samples showing symptoms consisting of bud proliferation and stunting at Experimental Farm, ICAR-NBPGR, New Delhi during Kharif 2020. Associated phytoplasma was characterized based on sequence analysis of target 16S rDNA fragments of 1247 bp amplified from symptomatic samples of cowpea by nested-polymerase chain reaction assay using universal phytoplasma specific primers pairs (P1/P7 and R16F2n/ R16R2). The 16S rDNA sequences understudy shared 98.6% similarity with that of the Candidatus Phytoplasma aurantifolia (GenBank accession: U15442). In phylogenetic analysis, the target 16S rDNA fragment from two samples (Cow-1 and Cow-2) clustered with strains of the 16SrII group of Ca. Phytoplasma aurantifolia. The virtual RFLP pattern showed the highest similarity with the reference pattern of the 16SrII-D subgroup (GenBank accession: Y10097) with a coefficient of 0.97. Since obtained coefficient value is equal to the threshold (0.97) for designating a different strain. Therefore, this phytoplasma infecting cowpea is proposed to be a new strain of Ca. P. aurantifolia as 16SrII-Ysubgroup.

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“…In India, C. sativa has already been reported as natural hosts of 16SrI, 16SrII, 16SrVI and 16SrXIV groups of phytoplasma (Raj et al 2008a, b;Mall 2009;Chaube et al 2015;Kumar et al 2017). However, P. hysterophorus was confirmed as natural host of 16SrI and 16SrII group phytoplasmas in India (Raj et al 2008b;Thorat et al 2016). In the present study, these two weed species C. sativa and P. hysterophorus were again confirmed as host of 16SrI-B and 16SrII-D phytoplasma subgroups, which were also identified on ornamentals (jasmine and chrysanthemum) in the present study.…”

Section: Discussionmentioning

confidence: 98%

Molecular characterization of phytoplasma associated with four important ornamental plant species in India and identification of natural potential spread sources

Gopala

Rao

2018

3 Biotech

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Phytoplasma suspected symptoms of phyllody, witches' broom, leaf yellowing, stunting and little leaf were observed in and during survey of flower nurseries and experimental ornamental fields at Delhi, Maharashtra, Tamil Nadu and Karnataka from 2014 to 2016. Pleomorphic bodies typical to phytoplasma structures were observed in the phloem sieve elements of ultrathin sections of all the four symptomatic ornamental plants (stem tissue) in transmission electron microscope. Amplification of 1.8 and 1.2 kb phytoplasma DNA products was observed in all the four test plants in PCR assays using universal primer pairs P1/P7 followed by nested primer pair R16F2n/R16R2, respectively. Pairwise sequence comparison, phylogeny and virtual RFLP analysis of 16S rDNA sequences confirmed the association of two phytoplasma subgroups (16SrI-B and 16SrII-D) in four ornamental plant species. '' subgroup D (16SrII-D) was found associated with chrysanthemum phyllody and leaf yellowing at Delhi and Tamil Nadu, bougainvillea little leaf and yellowing at Delhi and Chinese aster phyllody at Bengaluru, Karnataka. However, jasmine little leaf and yellowing at Bengaluru, Karnataka and chrysanthemum stunting at Pune were found to be associated with '. ' subgroup B-related strains (16SrI-B). The identification of 16SrII-D subgroup phytoplasma infecting bougainvillea and 16SrI-B subgroup infecting jasmine are the new reports to the world. Besides weed species, showing witches' broom in jasmine fields at Bengaluru and showing witches' broom symptoms in chrysanthemum fields at Delhi were identified to be caused by phytoplasma strains classified under subgroups 16SrI-B and 16SrII-D, respectively, by PCR assays and 16Sr DNA sequence comparison analysis. Among the three major leafhopper species identified, only was identified positive for 16SrI-B and 16SrII-D subgroups of phytoplasmas from chrysanthemum fields at Delhi and jasmine fields at Bengaluru, respectively. The identity of similar phytoplasma strains infecting ornamental species in leafhopper and the weed species in the present study suggested that and weeds may act as potential natural sources for secondary spread of the identified phytoplasma strains.

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Alternative weed hosts harbors 16SrII group phytoplasmas associated with little leaf and witches’ broom diseases of various crops in India

Cited by 22 publications

References 0 publications

Prevalence and genetic diversity of phytoplasmas infecting different crops in Egypt

Prevalence and genetic diversity of phytoplasmas infecting different crops in Egypt

Characterization of a novel strain of Candidatus Phytoplasma aurantifolia infecting cowpea (Vigna unguiculata) based on 16S rDNA sequence analysis

Molecular characterization of phytoplasma associated with four important ornamental plant species in India and identification of natural potential spread sources

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