Alternative Use of Extracts of Chipilín Leaves (Crotalaria longirostrata Hook. & Arn) as Antimicrobial

Abstract

Abstract:The genus Crotalaria comprises about 600 species that are distributed throughout the tropics and subtropical regions of the world; they are antagonistic to nematodes in sustainable crop production systems, and have also shown antimicrobial capacity. Chipilín (C. longirostrata), which belongs to this genus, is a wild plant that grows in the state of Chiapas (Mexico) and is traditionally is used as food. Its leaves also have medicinal properties and are used as hypnotics and narcotics; however, the plant has received little research attention to date. In the experimental part of this study, dried leaves were macerated by ethanol. The extract obtained was fractionated with ethyl ether, dichloromethane, ethyl acetate, 2-propanone, and water. The extracts were evaluated against three bacteria-namely, Escherichia coli (Ec), Citrobacter freundii (Cf), and Staphylococcus epidermidis (Se)-and three fungi-Fusarium oxysporum A. comiteca (FoC), Fusarium oxysporum A. tequilana (FoT), and Fusarium solani A. comiteca (FSC). During this preliminary study, a statistical analysis of the data showed that there is a significant difference between the control ciprofloxacin (antibacterial), the antifungal activity experiments (water was used as a negative control), and the fractions used. The aqueous fraction (WF) was the most active against FoC, FsC, and FoT (30.65, 20.61,and 27.36% at 96 h, respectively) and the ethyl ether fraction (EEF) was the most active against Se (26.62% at 48 h).

In Vitro Antifungal Activity and Chemical Composition of Piper auritum Kunth Essential Oil against Fusarium oxysporum and Fusarium equiseti

“…1 filter paper discs were placed to promote the growth of the phytopathogen on them. Mycelial discs were used as inoculum in all bioassays [27].…”

Edible Leafy Plants from Mexico as Sources of Antioxidant Compounds, and Their Nutritional, Nutraceutical and Antimicrobial Potential: A Review

“…Crotalaria longirostrata has antibacterial effects against Escherichia coli, Citrobacter freundii and Staphylococcus epidermidis and antifungal effects against Fusarium oxysporum A. comiteca, Fusarium oxysporum A. tequilana and Fusarium solani A. comiteca. The antifungal activity of C. longirostrata is attributed to phenolic compounds, alkaloids, essential oils and glycosides [ 108 ]. Suaeda nigra shows inhibitory effects on gram-positive strains (Bacillus cereus ATCC11778, B. subtilis ATCC6633, Staphylococcus aureus ATCC29737 and Corynebacterium rubrum ATCC14898) and gram-negative bacteria (Escherichia coli NCIM2931, Pseudomonas aeruginosa ATCC9027, Salmonella typhimurium ATCC23564 and Klebsiella pneumonia NCIM2719) and has antifungal effects against Candida albicans ATCC209l, Cryptococcus neoformans ATCC34664, Candida glabrata NCIM3448 and Candida apicola NCIM3367 [ 109 ].…”

Edible seeds, leaves and flowers as Maya Super Foods: Function and composition

“…40 The leaves have antioxidant and sedative activity, as well as antifungal activity to pytopathogens. 40,41 It contains nontoxic alkaloids, polyphenols, flavonoids, minerals (Mg, Fe, Zn) 32 , amino acids and vitamin C. 42 Dysphania ambrosioides (L.) Mosyakin & Clemants (Amaranthaceae) [Apazote, wormwood]. Strongly odorous herb aromatic leaves of which amply used in Mesoamerican cuisine.…”