Alternative splicing signature of alveolar type II epithelial cells of Tibetan pigs under hypoxia-induced

Abstract

Alternative splicing (AS) allows the generation of multiple transcript variants from a single gene and a ects biological processes by generating protein diversity in organisms. In total, , AS events corresponding to , genes were identified, and SE is the most abundant alternatively spliced type. The analysis of functional categories demonstrates that alternatively spliced di erentially expressed genes (DEGs) were enriched in the MAPK signaling pathway and hypoxia-inducible factor (HIF-) signaling pathway. Proteoglycans in cancer between the normoxic ( % O , TN and LN) and hypoxic ( % O , TL and LL) groups, such as SLC A , HK , HK , ENO , and PFKFB , have the potential to rapidly proliferate alveolar type II epithelial (ATII) cells by increasing the intracellular levels of glucose and quickly divert to anabolic pathways by glycolysis intermediates under hypoxia. ACADL, EHHADH, and CPT A undergo one or two AS types with di erent frequencies in ATII cells between TN and TL groups (excluding alternatively spliced DEGs shared between normoxic and hypoxic groups), and a constant supply of lipids might be obtained either from the circulation or de novo synthesis for better growth of ATII cells under hypoxia condition. MCM and MCM undergo di erent AS types between LN and LL groups (excluding alternatively spliced DEGs shared between normoxic and hypoxic groups), which may bind to the amino-terminal PER-SIM-ARNT domain and the carboxyl terminus of HIFα to maintain their stability. Overall, AS and expression levels of candidate mRNAs between Tibetan pigs and Landrace pigs revealed by RNA-seq suggest their potential involvement in the ATII cells grown under hypoxia conditions.

Exploring the role of the CapG gene in hypoxia adaptation in Tibetan pigs

“…Consequently, Tibetan pigs have been extensively studied as a significant food source for high-altitude Tibetan communities, given their ability to adapt to the plateau's hypoxic conditions. The prolonged exposure and evolution in this hypoxic region have contributed to the development of unique plateau characteristics and a stable genetic mechanism for hypoxia adaptation (Zhang et al, 2017;Yuan et al, 2022). Thus, Tibetan pigs are ideal subjects for studying the mechanisms of hypoxia adaptation.…”