Comparative analysis of PacBio and ONT RNA sequencing methods for Nemopilema Nomurai venom identification

Ma, Yuzhen; Li, Jie; Yu, Huahua; Teng, Lichao; Geng, Hao; Li, Rongfeng; Xing, Ronge; Liu, Song; Li, Pengcheng

doi:10.1016/j.ygeno.2023.110709

Genomics

2023

DOI: 10.1016/j.ygeno.2023.110709

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Comparative analysis of PacBio and ONT RNA sequencing methods for Nemopilema Nomurai venom identification

Yuzhen Ma,

Jie Li,

Huahua Yu

et al.

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2024

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Comparative Analysis of Tentacle Extract and Nematocyst Venom: Toxicity, Mechanism, and Potential Intervention in the Giant Jellyfish Nemopilema nomurai

Geng,

Wang,

Hou

et al. 2024

Marine Drugs

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The giant jellyfish Nemopilema nomurai sting can cause local and systemic reactions; however, comparative analysis of the tentacle extract (TE) and nematocyst venom extract (NV), and its toxicity, mechanism, and potential intervention are still limited. This study compared venom from TE and NV for their composition, toxicity, and efficacy in vitro and in vivo used RAW264.7 cells and ICR mice. A total of 239 and 225 toxin proteins were identified in TE and NV by proteomics, respectively. Pathological analysis revealed that TE and NV caused heart and liver damage through apoptosis, necrosis, and inflammation, while TE exhibited higher toxicity ex vivo and in vivo. Biochemical markers indicated TE and NV elevated creatine kinase, lactatedehydrogenase, and aspartate aminotransferase, with the TE group showing a more significant increase. Transcriptomics and Western blotting indicated both venoms increased cytokines expression and MAPK signaling pathways. Additionally, 1 mg/kg PACOCF3 (the phospholipase A2 inhibitor) improved survival from 16.7% to 75% in mice. Our results indicate that different extraction methods impact venom activities, tentacle autolysis preserves toxin proteins and their toxicity, and PACOCF3 is a potential antidote, which establishes a good extraction method of jellyfish venom, expands our understanding of jellyfish toxicity, mechanism, and provides a promising intervention.

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Comparative Analysis of Tentacle Extract and Nematocyst Venom: Toxicity, Mechanism, and Potential Intervention in the Giant Jellyfish Nemopilema nomurai

Geng,

Wang,

Hou

et al. 2024

Marine Drugs

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Comparative analysis of PacBio and ONT RNA sequencing methods for Nemopilema Nomurai venom identification

Cited by 1 publication

References 64 publications

Comparative Analysis of Tentacle Extract and Nematocyst Venom: Toxicity, Mechanism, and Potential Intervention in the Giant Jellyfish Nemopilema nomurai

Comparative Analysis of Tentacle Extract and Nematocyst Venom: Toxicity, Mechanism, and Potential Intervention in the Giant Jellyfish Nemopilema nomurai

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