Alternative medium for production of Pleurotus ostreatus biomass and potential antitumor polysaccharides

Gern, Regina Maria Miranda; Wisbeck, Elisabeth; Rampinelli, Jamile Rosa; Ninow, Jorge Luiz; Furlan, Sandra Aparecida

doi:10.1016/j.biortech.2006.11.059

Cited by 81 publications

(77 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Mushrooms have been appreciated and consumed for many years, by oriental cultures [9,24]. They are considered delicate in terms of texture, with characteristic color and pleasant Xavor [1][2][3][4][5][6][7][8][9][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Production of Pleurotus sajor-caju strain PS-2001 biomass in submerged culture

Confortin

Marchetto

Bettin

et al. 2008

J Ind Microbiol Biotechnol

View full text Add to dashboard Cite

Mushrooms or fruiting bodies of many basidiomycetes are commonly produced in solid-state fermentation, generally after 20-60 days of growth. However, it is also possible to produce biomass from these fungi, in submerged fermentation in shorter time. This work was aimed at evaluating biomass production with the basidiomycete Pleurotus sajor-caju, in a submerged process and to determine the proportion of chemical components of this biomass. Initially, an optimization of the culture medium was done to produce a faster growth of microbial mass by changing the concentrations of ammonium sulfate, soy protein and yeast extract. Using the optimized culture medium, values of approximately 5.5 g L(-1) of biomass in a medium with 10 g L(-1) of glucose were attained. When the optimized culture medium was tested in a 5-L stirred tank bioreactor, using 10 g L(-1) of glucose or sucrose as carbon source, values of 8.18 and 5.94 g L(-1) of biomass concentration were obtained, respectively. In the medium with glucose, high yields (0.82 g g(-1)) and productivity of 0.085 g L(-1) h(-1) were obtained. The exopolysaccharide content (1.58 g dry matter L(-1)) in the culture was higher in the fermentation with sucrose. The nutritional composition of the biomass obtained in the submerged fermentation was similar to that of the fruiting body in terms of quantities of total carbohydrates, ash and calories, but total fat and protein were higher.

show abstract

Section: Introductionmentioning

confidence: 99%

Production of Pleurotus sajor-caju strain PS-2001 biomass in submerged culture

Confortin

Marchetto

Bettin

et al. 2008

J Ind Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…The best conditions and composition of the culture medium for biomass and polysaccharide production from Pleurotus ostreatus in submerged cultures were evaluated by Gern et al (2008). Therefore, a 2 4 factorial design was performed in order to evaluate the influence of oxygen transfer coefficient (k L a) (10.2 or 19.3 h −1 ), glucose concentration (20 or 40 g/l), wheat extract supplemented with yeast extract (YE) (2 or 5 g/l) or with corn steep liquor (CSL), both used as organic nitrogen source (10 or 20 g/l), and ammonium sulphate as inorganic nitrogen source (0 or 5 g/l).…”

Section: Sparassis Crispamentioning

confidence: 99%

β-(1→3),(1→6)-Glucans: medicinal activities, characterization, biosynthesis and new horizons

Dalonso

Goldman

Gern

2015

Appl Microbiol Biotechnol

Self Cite

View full text Add to dashboard Cite

Biological activities of medicinal mushrooms have been attributed to β-(1→3),(1→6)-glucans that are present in the cell wall of fungi and some plants. Antitumor, immunomodulatory, antimicrobial, antinociception, antiinflammatory, prebiotic, antioxidant, and antidiabetic are some of different properties already described for β-(1→3),(1→6)-glucans. Immune activation systems, including specific β-glucan receptors like Dectin-1, complement (CR3), and Toll (TLR), have been identified to clarify these biological effects. The β-(1→3)-glucans are synthesized by β-(1→3)-glucan synthase (GLS), an enzyme belonging to the glucosyltransferase group, which has a catalytic unit (FKS) and another regulatory (RHO). The mechanisms for adding β-(1→6) branches to the non-reducing ends of the β-(1→3)-glucan chains are unclear until now. Due to the biological importance of β-(1→3),(1→6)-glucan, it is necessary to understand the biochemical and molecular mechanisms of its synthesis, both to optimize the production of bioactive compounds and to develop antifungal drugs that interrupt this process. Therefore, the aim of this review is to gather information about the potential of β-(1→3),(1→6)-glucans, their methods of isolation, purification, and chemical characterization, as well as how these biomolecules are synthesized by fungi and what studies involving biotechnology or molecular biology have contributed to this subject.

show abstract

“…For instance, the fruiting body and exopolysaccharides produced in submerged culture of this fungus have attracted considerable attention due to their biological properties including anti-tumor, anti-oxidant, immunostimulatory and cell proliferation effects [15][16][17][18][19][20]. Thus, some investigators have been focused on optimization of culture conditions in flask culture and bioreactors [21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Production of Liquiritigenin with Cell-based Biotransformation and Its Anti-Aging Activity

Hwang¹,

Jeong²,

Park

2015

KSBB Journal

View full text Add to dashboard Cite

In this study, an efficient whole cell-based biotransformation for the production of liquiritigenin was developed using Laetiporus sulphureus CS0218 as biocatalyst and aqueous extracts of Glycyrrhiza uralensis as co-substrate, respectively. In order to determine the efficacy of this method, the optimal bioconversion conditions including mycelial growth, three important enzyme activities (β-glucosidase, α-rhamnosidase and β-xylosidase), and apparent viscosity of culture broth were monitored. After optimization, aqueous extracts of G. uralensis were added to the culture medium to directly produce algycone liquiritigenin. By applying this strategy, 67.5% of liquiritin was converted to liquiritigenin at pH 3.0 after 9 days of incubation and finally liquiritigenin was purified from the reaction mixture. And then, their biological activities including anti-oxidant and superoxide dismutase were observed. In fact, purified liquiritigenin was capable of bi-directional functions (i.e., either up-regulation or down-regulation of SIRT1 which is associated with aging). The results indicate that this strategy would be beneficial to produce biologically active liquiritigenin and could be used in pharmaceutical, cosmetic and food applications.

show abstract

Alternative medium for production of Pleurotus ostreatus biomass and potential antitumor polysaccharides

Cited by 81 publications

References 25 publications

Production of Pleurotus sajor-caju strain PS-2001 biomass in submerged culture

Production of Pleurotus sajor-caju strain PS-2001 biomass in submerged culture

β-(1→3),(1→6)-Glucans: medicinal activities, characterization, biosynthesis and new horizons

Production of Liquiritigenin with Cell-based Biotransformation and Its Anti-Aging Activity

Contact Info

Product

Resources

About