Alternative Host Model To Evaluate
            <i>Aeromonas</i>
            Virulence

Froquet, Romain Bruno; Cherix, Nathalie; Burr, Sarah E.; Frey, Joachim; Vilches, Silvia; Tomás, Juan M.; Cosson, Pierre

doi:10.1128/aem.00908-07

Cited by 48 publications

(40 citation statements)

References 18 publications

(13 reference statements)

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“…5A and B) (14,17,21,33). As with other pathogenic bacteria (54,(83)(84)(85)(86), Dictyostelium was not able to feed on a lawn of F. noatunensis subsp. noatunensis.…”

Section: Discussionmentioning

confidence: 94%

Dissection of Francisella-Host Cell Interactions in Dictyostelium discoideum

Lampe

Brenz

Herrmann

et al. 2016

Appl Environ Microbiol

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f Francisella bacteria cause severe disease in both vertebrates and invertebrates and include one of the most infectious human pathogens. Mammalian cell lines have mainly been used to study the mechanisms by which Francisella manipulates its host to replicate within a large variety of hosts and cell types, including macrophages. Here, we describe the establishment of a genetically and biochemically tractable infection model: the amoeba Dictyostelium discoideum combined with the fish pathogen Francisella noatunensis subsp. noatunensis. Phagocytosed F. noatunensis subsp. noatunensis interacts with the endosomal pathway and escapes further phagosomal maturation by translocating into the host cell cytosol. F. noatunensis subsp. noatunensis lacking IglC, a known virulence determinant required for Francisella intracellular replication, follows the normal phagosomal maturation and does not grow in Dictyostelium. The attenuation of the F. noatunensis subsp. noatunensis ⌬iglC mutant was confirmed in a zebrafish embryo model, where growth of F. noatunensis subsp. noatunensis ⌬iglC was restricted. In Dictyostelium, F. noatunensis subsp. noatunensis interacts with the autophagic machinery. The intracellular bacteria colocalize with autophagic markers, and when autophagy is impaired (Dictyostelium ⌬atg1), F. noatunensis subsp. noatunensis accumulates within Dictyostelium cells. Altogether, the Dictyostelium-F. noatunensis subsp. noatunensis infection model recapitulates the course of infection described in other host systems. The genetic and biochemical tractability of the system allows new approaches to elucidate the dynamic interactions between pathogenic Francisella and its host organism.

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“…5A and B) (14,17,21,33). As with other pathogenic bacteria (54,(83)(84)(85)(86), Dictyostelium was not able to feed on a lawn of F. noatunensis subsp. noatunensis.…”

Section: Discussionmentioning

confidence: 94%

Dissection of Francisella-Host Cell Interactions in Dictyostelium discoideum

Lampe

Brenz

Herrmann

et al. 2016

Appl Environ Microbiol

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“…Use of the unicellular amoeba Dictyostelium to assess virulence by the growth of this organism on lawns of bacteria has been suggested (98). Virulent strains of A. salmonicida and A. hydrophila are nonpermissive (no plaques), while an avirulent mutant strain of A. hydrophila with a TTSS defect was found to be permissive (plaques).…”

Section: Animal Models Of Infectionmentioning

confidence: 99%

The GenusAeromonas: Taxonomy, Pathogenicity, and Infection

2010

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SUMMARY Over the past decade, the genus Aeromonas has undergone a number of significant changes of practical importance to clinical microbiologists and scientists alike. In parallel with the molecular revolution in microbiology, several new species have been identified on a phylogenetic basis, and the genome of the type species, A. hydrophila ATCC 7966, has been sequenced. In addition to established disease associations, Aeromonas has been shown to be a significant cause of infections associated with natural disasters (hurricanes, tsunamis, and earthquakes) and has been linked to emerging or new illnesses, including near-drowning events, prostatitis, and hemolytic-uremic syndrome. Despite these achievements, issues still remain regarding the role that Aeromonas plays in bacterial gastroenteritis, the extent to which species identification should be attempted in the clinical laboratory, and laboratory reporting of test results from contaminated body sites containing aeromonads. This article provides an extensive review of these topics, in addition to others, such as taxonomic issues, microbial pathogenicity, and antimicrobial resistance markers.

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“…However, mice are not appropriate hosts for pathogens such as Aeromonas as these normally infect cold-blooded species living at low temperatures [5]. Recently, it has been reported that zebrafish is an excellent model for assessing the LD 50 of Aeromonas hydrophila strains [6] and for examining host immune responses [7], but this model is not suitable for studying host-pathogen interactions at the molecular level [8].…”

Section: Introductionmentioning

confidence: 99%

“…The Dictyostelium amoeba has been used as an alternative host to evaluate Aeromonas virulence [5]. However, Benghezal et al .…”

Section: Introductionmentioning

confidence: 99%

Tetrahymena: An Alternative Model Host for Evaluating Virulence of Aeromonas Strains

Pang

Lin

et al. 2012

PLoS ONE

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An easier assessment model would be helpful for high-throughput screening of Aeromonas virulence. The previous study indicated the potential of Tetrahymena as a permissive model to examine virulence of Aeromonas hydrophila. Here our aim was to assess virulence of Aeromonas spp. using two model hosts, a zebrafish assay and Tetrahymena-Aeromonas co-culture, and to examine whether data from the Tetrahymena thermophila model reflects infections in the well-established animal model. First, virulence of 39 Aeromonas strains was assessed by determining the 50% lethal dose (LD50) in zebrafish. LD50 values ranging from 1.3×102 to 3.0×107 indicated that these strains represent a high to moderate degree of virulence and could be useful to assess virulence in the Tetrahymena model. In Tetrahymena-Aeromonas co-culture, we evaluated the virulence of Aeromonas by detecting relative survival of Aeromonas and Tetrahymena. An Aeromonas isolate was considered virulent when its relative survival was greater than 60%, while the Aeromonas isolate was considered avirulent if its relative survival was below 40%. When relative survival of T. thermophila was lower than 40% after co-culture with an Aeromonas isolate, the bacterial strain was regarded as virulent. In contrast, the strain was classified as avirulent if relative survival of T. thermophila was greater than 50%. Encouragingly, data from the 39 Aeromonas strains showed good correlation in zebrafish and Tetrahymena-Aeromonas co-culture models. The results provide sufficient data to demonstrate that Tetrahymena can be a comparable alternative to zebrafish for determining the virulence of Aeromonas isolates.

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Alternative Host Model To Evaluate Aeromonas Virulence

Cited by 48 publications

References 18 publications

Dissection of Francisella-Host Cell Interactions in Dictyostelium discoideum

Dissection of Francisella-Host Cell Interactions in Dictyostelium discoideum

The GenusAeromonas: Taxonomy, Pathogenicity, and Infection

Tetrahymena: An Alternative Model Host for Evaluating Virulence of Aeromonas Strains

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