Alternative Estimation of Human Exposure of Single-Walled Carbon                Nanotubes Using Three-Dimensional Tissue-Engineered Human Lung

Stoker, E.; Purser, Forrest; Kwon, Soonjo; Park, Young Bin; Lee, Joon Sang

doi:10.1080/10915810802552138

Cited by 19 publications

(15 citation statements)

References 41 publications

(61 reference statements)

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“…Another important cellular process is proliferation of MSCs represented by DNA concentration in MSCs exposed to different CNT Scaffolds. The MSC cell proliferation was related with type of MWCNT-collagen scaffold as shown in Figure 3.9 [43]. * significantly different than day 0 control (p < 0.05).…”

Section: Mscs Differentiation and Proliferation On Different Types Ofmentioning

confidence: 90%

“…In following the section we will describe an account of the inflammation testing protocol of alveolar epithelial cells and mechanism of CNT toxicity in lung fibroblasts initially reported by Stoker et al [43].…”

Section: In Vivo Studies On Cnt Toxicitymentioning

confidence: 99%

“…Recently, Stoker et al reported a simple evaluation method to test the toxicity of engineered CNTs. Cytotoxic/ inflammatory responses and barrier function of the human lung layers following exposure of CNTs were observed using in vitro a co-culture system of airways [43]. Kwon et al (2009) further established the role of Nitric oxide (NO) produced by many cells in the body.…”

Section: Inflammatory Mechanism Of Cnt Cytoxicitymentioning

confidence: 99%

“…The progress of osteogenesis was sensitive to released alkaline phosphatase concentration from MSC cells in medium [57]. Alkaline phosphatase enzyme concentration serves as biomarker of MSC differention and osteogenesis to compare the quality and biocompatibility of MW-CNTs in scaffold support material as shown in Figure 3.8 [43,57]. Another important cellular process is proliferation of MSCs represented by DNA concentration in MSCs exposed to different CNT Scaffolds.…”

Section: Mscs Differentiation and Proliferation On Different Types Ofmentioning

confidence: 99%

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Carbon Nanotubes: Nanotoxicity Testing and Bioapplications

Sharma¹,

Kwon²

2013

Nanomaterials in Drug Delivery, Imaging, and Tissue Engineering

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With the growing use of nanomaterials in bioapplications, the nanotoxicity of new nanomaterials has become a safety concern when used in various applications. In this chapter, technical developments on carbon nanotubes are described including a historical account, experimental models and potential bioapplications.Carbon nanotube (CNT) materials display superior properties in electric current carrying capacity, thermal conductivity, and thermal stability. Due to the unique CNT structure with high-aspect ratio, CNT may show unusual toxicity and complicate its safe use in a target tissue. To test nanotoxicity of CNT, we describe a set of protocols of prior knowledgebased physical and chemical characteristics to develop 3-dimensional in vitro models of the intact skin, and a 3D in vitro model of the human airway using a co-culture of normal human bronchial epithelial cells and normal human fibroblasts. The human airway 3D model served as a tool of health risk assessment of CNTs on the human respiratory systems. To test functionality at different CNT concentrations in a 3D model, physical characteristics of multiwalled CNTs and production of nitric oxide (NO) served as cell viability and inflammatory marker; mitochondrial activity (MTT assay) served as the cytotoxic response of the epithelial cell layers; transepithelial electrical resistance (TER) measured nanotoxicity in the changes in airway physiological function. Cytoxicity and inflammatory responses of CNTs were dependent on different size, mass, shape, and functionality of CNTs as viable in vivo tests were conducted to evaluate the Ashutosh Tiwari and Atul Tiwari (eds.) Nanomaterials in Drug Delivery, Imaging, and Tissue Engineering,

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Section: Mscs Differentiation and Proliferation On Different Types Ofmentioning

confidence: 90%

Section: In Vivo Studies On Cnt Toxicitymentioning

confidence: 99%

Section: Inflammatory Mechanism Of Cnt Cytoxicitymentioning

confidence: 99%

Section: Mscs Differentiation and Proliferation On Different Types Ofmentioning

confidence: 99%

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Carbon Nanotubes: Nanotoxicity Testing and Bioapplications

Sharma¹,

Kwon²

2013

Nanomaterials in Drug Delivery, Imaging, and Tissue Engineering

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“…Nanoparticles are known to stimulate pulmonary epithelial cells to produce inflammatory cytokines (117). Recently a 3D model of the human airways using a coculture of human bronchial and fibroblastic cells has been developed to investigate the effects and evaluate the risk assessment of carbon nanotubes (CNTs) on the respiratory system (143). Results have shown increased production of nitric oxide (inflammatory marker) and decreased cell viability as a function of CNT concentration in an aqueous solution.…”

Section: Tissue-engineered Lung Models In Drug Development and Discoverymentioning

confidence: 99%

Computational and bioengineered lungs as alternatives to whole animal, isolated organ, and cell-based lung models

Patel

Gauvin

Absar

et al. 2012

American Journal of Physiology-Lung Cellular and Molecular Phys

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Development of lung models for testing a drug substance or delivery system has been an intensive area of research. However, a model that mimics physiological and anatomical features of human lungs is yet to be established. Although in vitro lung models, developed and fine-tuned over the past few decades, were instrumental for the development of many commercially available drugs, they are suboptimal in reproducing the physiological microenvironment and complex anatomy of human lungs. Similarly, intersubject variability and high costs have been major limitations of using animals in the development and discovery of drugs used in the treatment of respiratory disorders. To address the complexity and limitations associated with in vivo and in vitro models, attempts have been made to develop in silico and tissue-engineered lung models that allow incorporation of various mechanical and biological factors that are otherwise difficult to reproduce in conventional cell or organ-based systems. The in silico models utilize the information obtained from in vitro and in vivo models and apply computational algorithms to incorporate multiple physiological parameters that can affect drug deposition, distribution, and disposition upon administration via the lungs. Bioengineered lungs, on the other hand, exhibit significant promise due to recent advances in stem or progenitor cell technologies. However, bioengineered approaches have met with limited success in terms of development of various components of the human respiratory system. In this review, we summarize the approaches used and advancements made toward the development of in silico and tissue-engineered lung models and discuss potential challenges associated with the development and efficacy of these models. bioengineered lung; computational modeling; in silico; lung models; tissue engineering LUNG DISEASES ARE THE THIRD leading cause of deaths in the United States that translate into one in six deaths. More than 400,000 Americans die of lung disease every year and around 35 million are now living with chronic lung problems (1). Therefore, there is an important need to understand as to how a new drug entity or a novel formulation may influence the anatomy, physiology, and pathogenesis of lungs and vice versa.The human lung has an approximate volume of six liters, contains about 300 million alveoli, and provides a total surface area of 100 square meters as blood-air interface, which is packed into an elastic dynamic structure responsible for gas exchange (159). The human airway wall is a connective tissue that includes smooth muscle cells (SMC), mucus glands, blood vessels, and fibroblasts surrounded by a collagen-rich extracellular matrix (ECM). Epithelial cells, responsible for the efficient oxygen and carbon dioxide transfer, are at the interface between air and the connective tissue and are attached to an underlying basement membrane. The respiratory system is the site of a variety of pulmonary diseases such as asthma, bronchitis, pneumonia, cystic fibrosis, emphysema, a...

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Lung tissue engineering: An update

Tebyanian

Karami

Nourani

et al. 2019

Journal Cellular Physiology

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Pulmonary disease is a worldwide public health problem that reduces the life quality and increases the need for hospital admissions as well as the risk of premature death.A common problem is the significant shortage of lungs for transplantation as well as patients must also take immunosuppressive drugs for the rest of their lives to keep the immune system from attacking transplanted organs. Recently, a new strategy has been proposed in the cellular engineering of lung tissue as decellularization approaches. The main components for the lung tissue engineering are: (1) A suitable biological or synthetic three-dimensional (3D) scaffold, (2) source of stem cells or cells, (3) growth factors required to drive cell differentiation and proliferation, and (4) bioreactor, a system that supports a 3D composite biologically active. Although a number of synthetic as well biological 3D scaffold suggested for lung tissue engineering, the current favorite scaffold is decellularized extracellular matrix scaffold. There are a large number of commercial and academic made bioreactors, the favor has been, the one easy to sterilize, physiologically stimuli and support active cell growth as well as clinically translational. The challenges would be to develop a functional lung will depend on the endothelialized microvascular network and alveolar-capillary surface area to exchange gas. A critical review of the each components of lung tissue engineering is presented, following an appraisal of the literature in the last 5 years. This is a multibillion dollar industry and consider unmet clinical need. K E Y W O R D S in vivo, lung, scaffold, stem cell, tissue engineering

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Alternative Estimation of Human Exposure of Single-Walled Carbon Nanotubes Using Three-Dimensional Tissue-Engineered Human Lung

Cited by 19 publications

References 41 publications

Carbon Nanotubes: Nanotoxicity Testing and Bioapplications

Carbon Nanotubes: Nanotoxicity Testing and Bioapplications

Computational and bioengineered lungs as alternatives to whole animal, isolated organ, and cell-based lung models

Lung tissue engineering: An update

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