Alterins, a new family of marine antibacterial cyclolipopeptides

Offret, Clément; Cuny, Héléna; Bodet, Pierre-Edouard; Desriac, Florie; Jégou, Camille; Bazire, Alexis; Chevrot, Romain; Thiéry, Valérie; Brillet, Benjamin; Fleury, Yannick

doi:10.1016/j.ijantimicag.2021.106514

Cited by 3 publications

(5 citation statements)

References 13 publications

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“…Indeed, strains hCg-42 and hOe-125 have been reported to produce alterins, which are cyclolipopeptides with demonstrated antimicrobial activity against several pathogens [22]. An improvement in survival rates had already been reported for the European abalone Haliotis tuberculata impregnated 4 h with the Pseudoalteromonas strain hCg-6 and then injected with V. harveyi ORM4 [19].…”

Section: Discussionmentioning

confidence: 97%

“…That study demonstrated that abalone immersed prior to infection in a seawater suspension of Pseudoalteromonas hCg-6 -isolated from the hemolymph of healthy marine mollusks [20] -showed signi cantly improved viability, with a 24% increase in the survival rate [19]. Pseudoalteromonas, well known to be associated with marine organisms, produce antimicrobial compounds such as alterins, which may contribute to host defense [21,22]. Alterins are considered to be one of the largest families of antibacterial cyclolipopeptides from marine bacteria [22].…”

Section: Introductionmentioning

confidence: 99%

“…Pseudoalteromonas, well known to be associated with marine organisms, produce antimicrobial compounds such as alterins, which may contribute to host defense [21,22]. Alterins are considered to be one of the largest families of antibacterial cyclolipopeptides from marine bacteria [22]. They are especially produced by Pseudoalteromonas strains hCg-6, hCg-42 and hOe-125.…”

Section: Introductionmentioning

confidence: 99%

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Potential of Marine Strains of Pseudoalteromonas to Improve Resistance of Juvenile Sea Bass to Pathogens and Limit Biofilm Development

Rahmani¹,

Parizadeh

Baud³

et al. 2023

Preprint

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The European sea bass (Dicentrarchus labrax) is one of the most produced marine fish species in Europe. Its larval stage is particularly sensitive and is thus characterized by relatively low survival rates, making it acutely vulnerable to multiple infectious hazards that can occur during its intensive production cycle in hatcheries and at sea. In this study, we investigated the potential probiotic effect of marine Pseudoalteromonas bacterial strains against two major pathogens of this species, Vibrio harveyi (a bacterial pathogen) and the nervous necrosis virus (NNV); we also investigated antibiofilm effect of these Pseudoalteromonas strains. Over an 8 to 12-week impregnation phase, seabass juveniles were immersed fortnightly for 4 hours in static hyperoxygenated seawater containing the probiotic candidates of Pseudoalteromonas strains at a concentration of 106 CFU/mL. We tested four candidates: (1) a combination of two strains producing antimicrobial compounds, hCg-42 and hOe-125; (2) strain 3J6, with known antibiofilm properties and (3) strain RA15, from the same genus, but with no identified probiotic effect. At the end of the impregnation phase, fish underwent an infection challenge with an intraperitoneal injection at a dose of 4.6 × 108 CFU/mL of V. harveyi or a 4-hour bath with a pathogenic strain of NNV. Thereafter, mortality was monitored for 2 and 6 weeks, respectively, at temperatures allowing the development of clinical signs. Immunological analyses were carried out during impregnation and after infection. The probiotic candidates were detected at different sampling times after the 4-hour immersion session in the gills and mucus, but there was no evidence of long-term persistence. For the V. harveyi challenge, no statistical difference in mortality was observed between the non-impregnated control (63%) and the 3J6-impregnated (68.7%) group, but improved survival rates of 10 and 25% were obtained for the RA15- and the double strain (hCg-42+hOe-125)-impregnated groups, respectively. For the NNV challenge, no significant benefic effect of the probiotics on infection kinetics or cumulative mortality was observed. Leucocyte mortality and phagocytosis activity revealed only slight significant differences between the treatment groups, either during impregnation or after infection challenges. Regarding biofilm development during impregnation with probiotic candidates, the maximal thickness of biofilm was significantly lower in the 3J6, double-strain and RA15 groups, compared with the non-impregnated control group. This study highlights the interesting probiotic potential of marine bacteria to limit mortalities induced by bacterial pathogens as well as biofilm development. Further investigations are in progress to investigate the mechanisms of action of these probiotics and to improve their formulation for larger-scale tests.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Potential of Marine Strains of Pseudoalteromonas to Improve Resistance of Juvenile Sea Bass to Pathogens and Limit Biofilm Development

Rahmani¹,

Parizadeh

Baud³

et al. 2023

Preprint

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“…The produced alterins belong to the family of cationic cyclo-lipopeptides, which bind to bacterial lipopolysaccharides, provoking membrane depolarization and subsequent cell permeability and lysis [113]. Similarly, Offret et al [114] obtained alterins from a group of 5 Pseudoalteromonas spp. isolated from the oyster hemolymph and also demonstrated ranging antibacterial activities against E. coli ATCC 25922, V. harveyi OMM4, V. pectenicida CIP 105190, V. tasmaniensis LGP32, and V. tapetis CECT 4600.…”

Section: Isatinmentioning

confidence: 99%

“…Provide the depolarization of the bacterial membrane and the subsequent cell permeabilization and lysis [113,114]. P. shioyasakiensis and P. mariniglutinosa.Poly-hydroxybutyrate-co hydroxy-hexanoate (PHBH) degrading abilities of certain gram-negative bacteria, including P. shioyasakiensis and P. mariniglutinosa.…”

mentioning

confidence: 99%

Therapeutic Potential of Marine Probiotics: A Survey on the Anticancer and Antibacterial Effects of Pseudoalteromonas spp.

et al. 2023

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Due to the increasing limitations and negative impacts of the current options for preventing and managing diseases, including chemotherapeutic drugs and radiation, alternative therapies are needed, especially ones utilizing and maximizing natural products (NPs). NPs abound with diverse bioactive primary and secondary metabolites and compounds with therapeutic properties. Marine probiotics are beneficial microorganisms that inhabit marine environments and can benefit their hosts by improving health, growth, and disease resistance. Several studies have shown they possess potential bioactive and therapeutic actions against diverse disease conditions, thus opening the way for possible exploitation of their benefits through their application. Pseudoalteromonas spp. are a widely distributed heterotrophic, flagellated, non-spore-forming, rod-shaped, and gram-negative marine probiotic bacteria species with reported therapeutic capabilities, including anti-cancer and -bacterial effects. This review discusses the basic concepts of marine probiotics and their therapeutic effects. Additionally, a survey of the anticancer and antibacterial effects of Pseudoalteromonas spp. is presented. Finally, marine probiotic production, advances, prospects, and future perspectives is presented.

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Potential of Marine Strains of Pseudoalteromonas to Improve Resistance of Juvenile Sea Bass to Pathogens and Limit Biofilm Development

Rahmani,

Parizadeh,

Baud

et al. 2023

Probiotics & Antimicro. Prot.

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Alterins, a new family of marine antibacterial cyclolipopeptides

Cited by 3 publications

References 13 publications

Potential of Marine Strains of Pseudoalteromonas to Improve Resistance of Juvenile Sea Bass to Pathogens and Limit Biofilm Development

Potential of Marine Strains of Pseudoalteromonas to Improve Resistance of Juvenile Sea Bass to Pathogens and Limit Biofilm Development

Therapeutic Potential of Marine Probiotics: A Survey on the Anticancer and Antibacterial Effects of Pseudoalteromonas spp.

Potential of Marine Strains of Pseudoalteromonas to Improve Resistance of Juvenile Sea Bass to Pathogens and Limit Biofilm Development

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