Alteribacter salitolerans sp. nov., isolated from a saline-alkaline soil

Wang, Shuang; Sun, Lei; Rao, Manik Prabhu Narsing; Liu, Guo-Hong; Jin, Pinjiao; Dong, Zhou-Yan; Lian, Zheng‐Han; Hao, Xiaoyu; Mingyi, Zhang; Li, Wenjun

doi:10.1007/s00203-021-02640-9

Cited by 2 publications

(4 citation statements)

References 46 publications

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“…Genomic DNA of strains APA_J-2 (6-2) T and APA_J-5 (13-2) was extracted with a TIANamp Genomic DNA kit (Tiangen Biotech). The 16S rRNA gene was amplified as described previously with the universal primers 27F (5′-AGAGTTTGATCCTGGCTCAG-3′) and 1492R (5′-CGGTTACCTTGTTACGACTT-3′) [8]. The PCR products were purified and cloned into the vector pMD19-T (Takara).…”

Section: S Rrna Gene Sequencing and Phylogenetic Analysismentioning

confidence: 99%

“…The tolerance of salinity and alkalinity were determined in liquid TSA medium with various NaCl concentrations (0, 2, 5, 8, 10, 15 and 20 %, w/v) and pH range (pH 6.0‒13.0, at intervals of 1.0 pH unit). The buffer system was prepared as described by Wang et al [8]. Growth was measured turbidometrically at 600 nm in a spectrophotometer.…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

“…After serial dilution of soil samples, the suspensions were plated on modified nutrient broth supplemented with 5 % (w/v) NaCl and 0.2 % (w/v) Na 2 CO 3 , followed by incubation at 37 °C for 2 days and subculturing to purity. Strains APA_J-2 (6-2) T and APA_J-5 (13-2) were transferred and purified on APA (Alkaline-Peptone-Agar) [8], and maintained as glycerol suspensions (20 %, v/v) at −80 °C for long-term preservation. The type strain of Oceanobacillus indicireducens JCM 17251 T was obtained from the Japan Collection of Microorganisms (JCM) for direct comparative taxonomic analyses.…”

Section: Isolation and Cultivationmentioning

confidence: 99%

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Oceanobacillus alkalisoli sp. nov., an alkaliphilic bacterium isolated from saline-alkaline soil

Jin

Sun

Rao

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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Two alkaliphilic strains, designated APA_J-2 (6-2)T and APA_J-5 (13-2), were isolated from saline-alkali soil sampled in Jilin Province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the two strains APA_J-2 (6-2)T and APA_J-5(13–2) were closely related to members of the genus Oceanobacillus , and had the highest sequence similarity to Oceanobacillus indicireducens JCM 17251T (96.8 and 96.9 %, respectively). The 16S rRNA gene sequence similarity between the two novel isolates was 99.6 %, indicating that they were similar species. Cells were Gram-stain-positive, aerobic, motile and rod-shaped. The strains grew at 15–45 °C (optimum, 37 °C), pH 8.0–11.0. (optimum, pH 9) and with 0–10 % (w/v) NaCl (optimum, 5 %). The strains contained menaquinone-7 as the respiratory quinone and anteiso-C15 : 0 as the predominant cellular fatty acid. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The genomic DNA G+C content was 40.0 mol%. The average nucleotide identity (ANI), amino acid identity (AAI) and digital DNA–DNA hybridization (dDDH) values of strain APA_J-2 (6-2)T with O. indicireducens JCM 17251T were 85.5, 87.9 and 30.7 %, respectively. The ANI, AAI and dDDH values of strain APA_J-5 (13-2) with O. indicireducens JCM 17251T were 85.7, 87.7 and 30.8 %, respectively. Based on the phylogenetic, phenotypic, biochemical, chemotaxonomic and genome data, strains APA_J-2 (6-2)T and APA_J-5 (13-2) represent a novel species of the genus Oceanobacillus , for which the name Oceanobacillus alkalisoli sp. nov. is proposed. The type strain is APA_J-2 (6-2)T (=KCTC 43253T=GDMCC 1.2242T).

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Section: S Rrna Gene Sequencing and Phylogenetic Analysismentioning

confidence: 99%

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

Section: Isolation and Cultivationmentioning

confidence: 99%

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Oceanobacillus alkalisoli sp. nov., an alkaliphilic bacterium isolated from saline-alkaline soil

Jin

Sun

Rao

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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“…The genus Alteribacter was proposed by Gupta et al [1] and according to the List of Prokaryotic Names with Standing in Nomenclature, there are currently three validly published species names [2] , Alteribacter aurantiacus , Alteribacter natronophilus and Alteribacter populi [1, 3–5]. At the time of writing, one more species of this genus, ‘ Alteribacter salitolerans’ , was reported by Wang et al [6]. During the proposal of the genus Alteribacter , Bacillus lacisalsi was also indicated to be a part of this clade [1].…”

Section: Full-textmentioning

confidence: 99%

Proposal to transfer Bacillus lacisalsi Dong et al. 2021 to the genus Alteribacter as Alteribacter lacisalsi comb. nov.

Rao

Wang

Banerjee

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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In the present study, the taxonomic position of Bacillus lacisalsi YSP-3T was evaluated using phylogenetic and genome-based comparison. B. lacisalsi YSP-3T showed the highest 16S rRNA gene sequence similarity to Alteribacter natronophilus M30T (98.4 %), followed by Alteribacter aurantiacus K1-5T (97.5 %) and Alteribacter populi FJAT-45347T (97.2 %). In phylogenetic (based on 16S rRNA gene sequences) and phylogenomic (based on 71 bacterial single-copy genes) trees, B. lacisalsi YSP-3T clustered with the members of the genus Alteribacter . The amino acid identity (AAI) values between B. lacisalsi YSP-3T and the members of the genus Alteribacter were >65 %, which is above the cut-off level (65–95 %) for genus delineation. The average nucleotide identity (ANI) values between B. lacisalsi YSP-3T and the members of the genus Alteribacter were <95 %, which is lower than the threshold value (95–96 %) for bacterial species delineation. The AAI value suggested that B. lacisalsi YSP-3T was a member of the genus Alteribacter while the ANIb value suggested it as a novel species of the genus Alteribacter . Based on the results, we propose to transfer Bacillus lacisalsi to the genus Alteribacter as Alteribacter lacisalsi comb. nov.

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Alteribacter salitolerans sp. nov., isolated from a saline-alkaline soil

Cited by 2 publications

References 46 publications

Oceanobacillus alkalisoli sp. nov., an alkaliphilic bacterium isolated from saline-alkaline soil

Oceanobacillus alkalisoli sp. nov., an alkaliphilic bacterium isolated from saline-alkaline soil

Proposal to transfer Bacillus lacisalsi Dong et al. 2021 to the genus Alteribacter as Alteribacter lacisalsi comb. nov.

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