Altered uterine contractility in women with chronic endometritis

Pinto, Vincenzo; Matteo, Maria; Tinelli, Raffaele; Mitola, Paola Carmela; Ziegler, Dominique de; Cicinelli, Ettore

doi:10.1016/j.fertnstert.2015.01.007

Cited by 63 publications

(42 citation statements)

References 24 publications

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“…A recent report showed that altered uterine contractility in CE patients leads to uterine dysperistalsis, which may be a significant cause of infertility [45]. In women with CE, an abnormal pattern of lymphocyte subsets and, consequently, an aberrant endometrial microenvironment have been demonstrated [46].…”

Section: Discussionmentioning

confidence: 99%

Chronic endometritis modifies decidualization in human endometrial stromal cells

Kimura

Zheng

et al. 2017

Reprod Biol Endocrinol

118

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BackgroundChronic endometritis (CE) is a continuous inflammation of uterine endometrium, and it is usually symptomless. As CE has been thought not to affect the reproductive status and general health of affected women, its significance has not been explored. However, recent studies have shown that CE is related with repeated implantation failures after in vitro fertilization-embryo transfer, unexplained infertility, and recurrent miscarriages. As decidua differentiates to support the implantation process and maintains the pregnancy, we hypothesized that CE may influence the process of decidualization.MethodsSeventeen patients were employed in the experiment involving culture of endometrial stromal cells (ESCs). After obtaining endometrial samples, ESCs were harvested and cultured for 13 days. The concentrations in culture media and the protein expressions in ESCs of prolactin (PRL) and insulin-like growth factor binding protein-1 (IGFBP-1), two well known decidualization markers used in a large number of in vitro models, were analyzed by ELISA and Western blotting, respectively, and the cell numbers were also counted. The mRNA levels of PRL and IGFBP-1 were tested by quantitative real time polymerase chain reaction (RT-PCR). Since sex hormone induce proliferation and differentiation to decidua via binding to the sex hormone receptors (ERα, ERβ, PRA, and PRB), their expression was assessed in another 17 patients’ paraffin-embedded endometrial tissue specimens by immunohistochemistry and semi-quantified by H-score.ResultsIncreased cell numbers and reduced secretion of PRL and IGFBP-1 were detected by ELISA in the ESCs of CE patients after culture for 13 days compared with non-CE patients. The decreased protein expression of IGFBP-1 in ESCs of CE patients was detected by Western blotting. The decreased expression of PRL mRNA and IGFBP-1 mRNA were detected by RT-PCR. Increased expressions of ERα, ERβ, PRA, and PRB were observed in the stromal cells of CE patients in comparison to non-CE patients, whereas increased expressions of ERα and ERβ were detected in the glandular cells of CE.ConclusionOur data suggests that CE modifies decidualization of human ESC through untuning the function of sex steroid hormone receptor.Electronic supplementary materialThe online version of this article (doi:10.1186/s12958-017-0233-x) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Chronic endometritis modifies decidualization in human endometrial stromal cells

Kimura

Zheng

et al. 2017

Reprod Biol Endocrinol

118

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“…It has been reported that women with CE showed altered uterine contractility in both the periovulatory and midluteal phases . This alteration may be associated with symptoms related to CE, such as pelvic pain, spotting and implantation failure.…”

Section: Pathophysiologymentioning

confidence: 99%

“…Although, histologically, CE is generally defined as the presence of any plasma cell in the stroma, some argue that CE should not be diagnosed by only a few plasma cells, because the endometrial stroma can contain a limited number of plasma cells without an inflammatory process. In fact, some investigators consider finding one plasma cell in the endometrial stroma as sufficient for diagnosis, although other investigators stated that more than five plasma cells in at least one of three sections is required . Third, the site of CE in the uterus is another concern.…”

Section: Diagnosismentioning

confidence: 99%

“…It has been reported that women with CE showed altered uterine contractility in both the periovulatory and midluteal phases. 57 This alteration may be associated with symptoms related to CE, such as pelvic pain, spotting and implantation failure. The authors speculate that CE could influence contractility, since abnormal lymphocyte subpopulations and the altered pattern of paracrine factors in the endometrium could affect the synchronized movement of the endometrium and myometrium, including the junctional zone.…”

Section: Pathophysiologymentioning

confidence: 99%

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Review: Chronic endometritis and its effect on reproduction

Kimura

Takebayashi

Ishida

et al. 2019

J of Obstet and Gynaecol

177

144

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Aim Chronic endometritis (CE) is a disease of continuous and subtle inflammation characterized by the infiltration of plasma cells in the endometrial stromal area. Although the clinical significance of CE has been thought in clinical practice for a long time because it is either asymptomatic or presents with subtle symptoms, recent studies have shown the potential adverse effects of CE on fertility. In the present review, we focus on the concept, diagnosis, etiology, pathophysiology, diagnosis, impact on reproduction and treatment for it to understand CE. Methods The published articles were reviewed. Results The prevalence of CE has been found to be 2.8–56.8% in infertile women, 14–67.5% in women with recurrent implantation failure (RIF), and 9.3–67.6% in women with recurrent pregnancy loss. Microorganisms are thought to be a main cause of CE, since antibiotic treatment has been reported to be an effective therapy for CE. Common bacteria are frequently detected in the uterine cavity of CE patients by microbial culture. In CE endometrium, the prevalence of immune cells and decidualization has been reported to be modified, and these modifications are thought to adversely affect fertility. The gold standard for the diagnosis of CE is the histological detection of plasma cells in the stromal area of the endometrium in endometrial specimens, although universally accepted criteria for the diagnosis of CE have not been determined. The treatment currently thought to be most effective for the recovery of fertility in CE is administration of oral antibiotics. Patients whose CE has been cured have been reported to have a higher ongoing pregnancy rate, clinical pregnancy rate, and implantation rate compared with patients with persistent CE. Conclusion CE greatly affects implantation and impairs fertility. Antibiotic administration is an effective therapeutic option. Pregnancy rate in in vitro fertilization is improved when CE is cured by antibiotic.

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“…У женщин репродуктивного возраста с верифи-цированным хроническим эндометритом выявляют-ся нарушения менструального цикла (47%) и репро-дуктивной функции (67%), бесплодие (60-87%), не-удачные попытки ЭКО (37%), невынашивание бере-менности (60,5-86,7%) [46,53].…”

Section: хронический эндометрит -возможная причина нарушений имплантаunclassified

Reproductive issues in HIV-infected couples and the possibility of their correction with the help of assisted reproductive technology (a review)

Medvedeva¹,

Malgina²

2016

Probl. reprod.

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1 ФГбу «уральский научно-исследовательский институт охраны материнства и младенчества» (дир. -д.м.н., проф. н.В. башмакова), екатеринбург, Россия, 620028;2 Ао «центр семейной медицины» (дир. -к.м.н. и.Г. Портнов), екатеринбург, Россия, 620043По данным ВоЗ, число инфицированных вирусом иммунодефицита человека (ВиЧ) растет с каждым годом. Первичная заболеваемость на территории России за последние 10 лет увеличилась в 3 раза. на сегодняшний день инфицированность населения ВиЧ составляет 461,6 на 100 000 человек. Доля инфицированных женского пола среди всех зарегистрированных в Российской Федерации составляет 36,6%, при этом максимальная заболеваемость зарегистрирована среди женщин в возрастной группе 25-34 года, т.е. в репродуктивном возрасте. однако инфицированность ВиЧ сегодня воспринимается не как смертельное состояние, а как терапевтически контролируемое хроническое заболевание. Социальный статус инфицированных находится на высоком уровне. Поэтому их желание иметь детей является закономерным. но около 15% из них не могут иметь спонтанную беременность. Проведен обзор современной литературы по данной проблеме. большинство авторов отмечают повышенную частоту патологии эякулята у сероположительных мужчин, что может являться причиной отсутствия беременности в серодискордантных либо конкордантных по ВиЧ парах. Существуют разные точки зрения на проблему гинекологической патологии у ВиЧ-положительных женщин, которые могут быть причиной репродуктивных неудач у данной категории женщин. В результате этого ВиЧ-инфицированные пары все чаще прибегают к программам вспомогательных репродуктивных технологий. По мнению большинства авторов, первое место в этиологии бесплодия занимает трубно-перитонеальный фактор. однако, по данных разных источников, эффективность программ у ВиЧ-инфицированных пар составляет не более 10%. нет единого мнения авторов относительно репродуктивных неудач. одной из причин неудачных попыток ЭКо у данной категории женщин можно считать развитие специфического эндометрита, не поддающегося стандартной терапии. Поэтому требуется разработка специальных алгоритмов подготовки данной категории пациентов к программам вспомогательных репродуктивных технологий (ВРТ). According to the world health organization, the number infected by human immunodeficiency virus (HiV) is growing every yearprimary morbidity in Russia in the last 10 years has increased almost in 3 times. to date, the HiV infection of the population was 502 at 1000 thousand people. the proportion of infected women among all registered in the Russian Federation is 36,6%, the maximal incidence was registered among women in the age group 25-34 years. However, the human immunodeficiency virus today, is not perceived as a fatal condition, and therapeutically it is a manageable chronic disease, social status of infected is high. therefore, their desire to have children is natural. but about 15% of them can't have a spontaneous pregnancy. the majority of authors note the increased frequency of pathology of ejaculate in seropositive men, which can cause lack of...

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Altered uterine contractility in women with chronic endometritis

Cited by 63 publications

References 24 publications

Chronic endometritis modifies decidualization in human endometrial stromal cells

Chronic endometritis modifies decidualization in human endometrial stromal cells

Review: Chronic endometritis and its effect on reproduction

Reproductive issues in HIV-infected couples and the possibility of their correction with the help of assisted reproductive technology (a review)

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