Altered sensitivity to nitric oxide donors, induced by intravascular infusion of quantum dots, in murine mesenteric arteries

Shukur, Ali; Rizvi, Sarwat; Whitehead, Debra; Seifalian, Alexander M.; Azzawi, May

doi:10.1016/j.nano.2012.10.004

Cited by 10 publications

(5 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This finding was supported by other studies from our group, in large vessels described earlier. In contrast, the in vivo injected SiNPs induced a significant reduction in SNP induced dilation, as we have previously demonstrated for the QDs (Shukur et al, 2013). The mechanism for this is unclear, however, it is noteworthy that ingestion of silica over an 8 day period has been documented to modify the characteristics of endothelial dilation in rat aorta, with signification reduction in the SNP induced dilation and attenuation of SMC responsiveness to nitric oxide (Oner et al, 2006).…”

Section: Discussionsupporting

confidence: 52%

The Influence of Silica Nanoparticles on Small Mesenteric Arterial Function

Shukur

Whitehead

Seifalian

et al. 2016

Nanomedicine (Lond.)

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 52%

The Influence of Silica Nanoparticles on Small Mesenteric Arterial Function

Shukur

Whitehead

Seifalian

et al. 2016

Nanomedicine (Lond.)

Self Cite

View full text Add to dashboard Cite

show abstract

“…Several mechanisms could be involved in the hypotensive effects of NO donors. We have previously reported that NDBP induces vasodilatation in mesenteric artery; similar to other NO donors (Araújo et al, 2013;Shukur et al, 2013), this effect was mediated by NO/cGMP/PKG pathway (França-Silva et al, 2012a). Centrally-mediated hypotensive effects of NO donors are also well documented: direct administration of NO or a NO donors into the paraventricular nucleus (PVN) decreases sympathetic nerve activity and lowers arterial blood pressure (Horn et al, 1994;Zhang and Patel, 1998).…”

Section: Discussionmentioning

confidence: 85%

Cardiorespiratory effects induced by 2-nitrate-1,3-dibuthoxypropan are reduced by nitric oxide scavenger in rats

Queiroz

Mendes-Júnior

Guimarães

et al. 2014

Autonomic Neuroscience

View full text Add to dashboard Cite

The search for new nitric oxide donors is warranted by the limitations of organic nitrates currently used in cardiology. The new organic nitrate 2-nitrate-1,3-dibuthoxypropan (NDBP) exhibited promising cardiovascular activities in previous studies. The aim of this study was to investigate the cardiorespiratory responses evoked by NDBP and to compare them to the clinically used organic nitrate nitroglycerine (NTG). Arterial pressure, heart rate and respiration were recorded in conscious adult male Wistar rats. Bolus i.v. injection of NDBP (1 to 15mg/kg; n=8) and NTG (0.1 to 5mg/kg; n=8) produced hypotension. NDBP induced bradycardia at all doses, while NTG induced tachycardia at three lower doses but bradycardia at higher doses. Hydroxocobalamin (20mg/kg; HDX), a NO scavenger, blunted hypotension induced by NDBP (15mg/kg), and its bradycardic effect (n=6). In addition, HDX blunted both hypotension and bradycardia induced by a single dose of NTG (2.5mg/kg; n=6). Both NDBP and NTG altered respiratory rate, inducing a biphasic effect with a bradypnea followed by a tachypnea; HDX attenuated these responses. Our data indicate that NDBP and NTG induce hypotension, bradycardia and bradypnea, which are mediated by nitric oxide release.

show abstract

“…AuNPs may interact directly with the SNP drug, as has been demonstrated previously in mesenteric vessels after incubation in quantum dots. 49 Such interaction has also been suggested for a number of other agonists after pulmonary NP exposure, 50 thus confirming the additional need for surface modification of AuNPs before use.…”

mentioning

confidence: 71%

Polyvinylpyrrolidone-coated gold nanoparticles inhibit endothelial cell viability, proliferation, and ERK1/2 phosphorylation and reduce the magnitude of endothelial-independent dilator responses in isolated aortic vessels

Mohamed¹,

Matou‐Nasri²,

Farooq³

et al. 2017

IJN

Self Cite

View full text Add to dashboard Cite

Background Gold nanoparticles (AuNPs) demonstrate clinical potential for drug delivery and imaging diagnostics. As AuNPs aggregate in physiological fluids, polymer-surface modifications are utilized to allow their stabilization and enhance their retention time in blood. However, the impact of AuNPs on blood vessel function remains poorly understood. In the present study, we investigated the effects of AuNPs and their stabilizers on endothelial cell (EC) and vasodilator function. Materials and methods Citrate-stabilized AuNPs (12±3 nm) were synthesized and surface-modified using mercapto polyethylene glycol (mPEG) and polyvinylpyrrolidone (PVP) polymers. Their uptake by isolated ECs and whole vessels was visualized using transmission electron microscopy and quantified using inductively coupled plasma mass spectrometry. Their biological effects on EC proliferation, viability, apoptosis, and the ERK1/2-signaling pathway were determined using automated cell counting, flow cytometry, and Western blotting, respectively. Endothelial-dependent and independent vasodilator functions were assessed using isolated murine aortic vessel rings ex vivo. Results AuNPs were located in endothelial endosomes within 30 minutes’ exposure, while their surface modification delayed this cellular uptake over time. After 24 hours’ exposure, all AuNPs (including polymer-modified AuNPs) induced apoptosis and decreased cell viability/proliferation. These inhibitory effects were lost after 48 hours’ exposure (except for the PVP-modified AuNPs). Furthermore, all AuNPs decreased acetylcholine (ACh)-induced phosphorylation of ERK1/2, a key signaling protein of cell function. mPEG-modified AuNPs had lower cytostatic effects than PVP-modified AuNPs. Citrate-stabilized AuNPs did not alter endothelial-dependent vasodilation induced by ACh, but attenuated endothelial-independent responses induced by sodium nitroprusside. PVP-modified AuNPs attenuated ACh-induced dilation, whereas mPEG-modified AuNPs did not, though this was dose-related. Conclusion We demonstrated that mPEG-modified AuNPs at a therapeutic dosage showed lower cytostatic effects and were less detrimental to vasodilator function than PVP-modified AuNPs, indicating greater potential as agents for diagnostic imaging and therapy.

show abstract

Altered sensitivity to nitric oxide donors, induced by intravascular infusion of quantum dots, in murine mesenteric arteries

Cited by 10 publications

References 26 publications

The Influence of Silica Nanoparticles on Small Mesenteric Arterial Function

The Influence of Silica Nanoparticles on Small Mesenteric Arterial Function

Cardiorespiratory effects induced by 2-nitrate-1,3-dibuthoxypropan are reduced by nitric oxide scavenger in rats

Polyvinylpyrrolidone-coated gold nanoparticles inhibit endothelial cell viability, proliferation, and ERK1/2 phosphorylation and reduce the magnitude of endothelial-independent dilator responses in isolated aortic vessels

Contact Info

Product

Resources

About