Altered Protein and Gene Expression of Beclin-1 Correlates with Poor Prognosis of Hcv-Associated Hepatocellular Carcinoma in Egyptian Patients

Ehsan, Nermine; Mosbeh, Asmaa; Elkhadry, Sally Waheed; Gomaa, Asmaa; Elsabaawy, Maha; El-Azab, Dina S

doi:10.31557/apjcp.2021.22.4.1115

Cited by 4 publications

(4 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When human cells inside and outside conditions change, autophagy can play a positive role, such as can include through the use of the material supplies for the degradation of the cell cycle, or through the cell material and organelles updates and regulation in order to maintain stability and balance in the body, through the clear cytoplasm and damaged organelles in cells in order to protect the damaged cells (20). Current studies have identified 34 autophagy-related genes and 18 autophagy-regulating genes (12,13,21). Under normal circumstances, LC3-II mainly exists on the surface of autophagy vesicles and participates in the formation of autophagosomes, which also leads to the expression of LC3, indicating the occurrence of autophagy (22).…”

Section: Discussionmentioning

confidence: 99%

“…However, the existing research results indicate that the application of Angelica Shaoyao powder can achieve a good effect for the etiology of blood deficiency and water deficiency in the late stage of acute pancreatitis (9). Autophagy, also known as type II programmed death, is an important way for cells to remove excess and damage organelles and provide energy for cell metabolism (10) and engage many cytogenetic events in cancer and malignant transformation process (10)(11)(12)(13)(14)(15). In recent years, some studies have shown that autophagy is closely related to the occurrence of acute pancreatitis, for example, Biczo et al (16) showed that mitochondrial dysfunction and damaged autophagy, as the main downstream effectors, play a central role in the development of AP.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect and mechanism of Angelic Shaoyaosan mediated AMPK/SIRT1 positive feedback loop to promote autophagy and regulate the systemic inflammatory response in acute pancreatitis

Sun

Nie

2021

Cell Mol Biol (Noisy-le-grand)

View full text Add to dashboard Cite

This research was carried out to investigate the effect and mechanism of Angelic Shaoyaosan mediated AMPK/SIRT1 positive feedback loop to promote autophagy and regulate systemic inflammatory response in acute pancreatitis. In this study, the rat pancreatic acini AR42J cells were chosen as the research object, the application of hyla induced pancreatic acinar cells made model for acute pancreatitis, application of different concentrations of angelica peony spread effect on building cells, thus divided into control group, built in the module, the low concentration group, concentration and high concentration groups, determined by MTT method was applied to explore the above categories in cell proliferation, cell apoptosis was measured by flow cytometry, the expression of inflammatory factors in cell supernatant was determined by enzyme-linked immunoassay, and the expression of autophagy marker proteins LC3- ? and P62 was determined by Western-Bolt method. In order to explore the relationship between AMPK and SIRT1, immunoco-precipitation method was used to determine the interaction between AMPK and SIRT1, and dual luciferase experiment was used to explore the effect of AMPK on SIRT1. The AICAR group, BLM-275 group and negative control group were established. To explore the effect of SIRT1 on AMPK, we established SRT 1720 group, EX-527 group and control group. Direct binding between AMPK and SIRT1 should be determined by chromatin co-precipitation assay. In order to further explore the effect of AMPK/SIRT1 positive feedback loop on the systemic inflammatory response of acute pancreatitis, this study selected the medium-concentration Danggui Shaoyajiao SAN group as the control group (group C), and applied AMPK inhibitor BLM-275 and SIRT1 inhibitor EX 527 to the effect of medium-concentration Danggui Shaoyajiao SAN cells, respectively. The expression of autophagy marker proteins LC3- ? and P62 in groups A and B were determined by the Western-Bolt method. Results showed that compared with the control group, the cell survival rate, the expression of AMPK, SIRT1 and LC3-II in the model group were decreased, and the apoptosis rate of iNOS, IL-2, TNF-?, P62 and apoptosis were increased in the model group (P<0.05). the levels of iNOS, IL-2, TNF-?, P62 and cell survival rate in low, medium and high concentration groups decreased gradually, while the expressions of AMPK, SIRT1, LC3-II and cell apoptosis rate increased (P<0.05). The levels of iNOS, IL-2 and TNF-? in the three groups were gradually decreased with the increase of the concentration (P<0.05). Immunoprecipitation showed that AMPK and SIRT1 could bind to each other in cells. The double luciferase experiment indicated that the reporter gene containing the SIRT1 binding site was constructed. The luciferase activity was increased in THE AICAR group and decreased in the BLM-275 group (P<0.05). The reporter gene containing the AMPK promoter binding site was constructed. The luciferase activity in SRT1720 group was increased, while that in EX-527 group was decreased. SIRT1 could directly bind to the AMPK promoter. SIRT1 and LC3- ? protein expressions in group A were down-regulated, and P62 protein was increased (P<0.05). The protein expressions of AMPK and LC3- ? in group B were down-regulated, and the protein expression of P62 was increased (P<0.05). It concluded that AMPK can directly bind to activate SIRT1 expression, and SIRT1 expression can also activate AMPK, forming a positive feedback loop between the two. Therefore, Angelic Shaoyaodong decoction can mediate AMPK/SIRT1 positive feedback pathway to promote autophagy and regulate systemic inflammatory response in acute pancreatitis.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect and mechanism of Angelic Shaoyaosan mediated AMPK/SIRT1 positive feedback loop to promote autophagy and regulate the systemic inflammatory response in acute pancreatitis

Sun

Nie

2021

Cell Mol Biol (Noisy-le-grand)

View full text Add to dashboard Cite

show abstract

“…When separating from Bcl-2, Beclin 1 can form PI3KC3 complex with Vps15, Vps34 (also called type III PI3K) and other proteins like Ambra1, to regulate the initiation of autophagy [ 42 ]. Studies have shown the downregulation of Beclin 1 and upregulation of Bcl-2 in many types of cancers, both were closely related to poor prognosis [ 91 , 92 ]. It has been reported that Bcl-2 antagonized the autophagy pathway, subverted the internal protein quality and genome stability and promoted the growth of breast cancer cells, indicating that Beclin 1 and Bcl-2 can be served as attractive targets for cancer therapy [ 93 ].…”

Section: Indirect Redox Regulation Of Autophagy In Cancermentioning

confidence: 99%

Redox Regulation of Autophagy in Cancer: Mechanism, Prevention and Therapy

Dong

Luo

et al. 2022

Life

View full text Add to dashboard Cite

Reactive oxygen species (ROS), products of normal cellular metabolism, play an important role in signal transduction. Autophagy is an intracellular degradation process in response to various stress conditions, such as nutritional deprivation, organelle damage and accumulation of abnormal proteins. ROS and autophagy both exhibit double-edged sword roles in the occurrence and development of cancer. Studies have shown that oxidative stress, as the converging point of these stimuli, is involved in the mechanical regulation of autophagy process. The regulation of ROS on autophagy can be roughly divided into indirect and direct methods. The indirect regulation of autophagy by ROS includes post-transcriptional and transcriptional modulation. ROS-mediated post-transcriptional regulation of autophagy includes the post-translational modifications and protein interactions of AMPK, Beclin 1, PI3K and other molecules, while transcriptional regulation mainly focuses on p62/Keap1/Nrf2 pathway. Notably, ROS can directly oxidize key autophagy proteins, such as ATG4 and p62, leading to the inhibition of autophagy pathway. In this review, we will elaborate the molecular mechanisms of redox regulation of autophagy in cancer, and discuss ROS- and autophagy-based therapeutic strategies for cancer treatment.

show abstract

“…With the rapid development of genetics, the genetics of stroke has gradually become a research hotspot in recent years. Studies have shown that genetic and environmental factors play an important role in the occurrence and development of stroke (4)(5)(6)(7)(8)(9). Genome-Wide Association Study (GWAS) based on single nucleotide polymorphisms (SNPs) is a powerful tool for studying complex diseases (10).…”

Section: Introductionmentioning

confidence: 99%

Common genetic variants on chromosome 9p21 confers risk of ischemic stroke: A large-scale genetic association study

Li¹,

Zhang²,

Yin³

et al. 2021

Cell Mol Biol (Noisy-le-grand)

View full text Add to dashboard Cite

The objective of this experiment was to observe the allele frequency and genotype distribution of some 9p21 SNPs in the Anhui Han population, and to study its relationship with the susceptibility to ischemic stroke. For this purpose, a collection of 992 patients with ischemic stroke confirmed and hospitalized in our hospital from October 2017 to October 2020 were used as the IS case group, and 951 normal people who had a healthy physical examination in the physical examination center of our hospital during the same period were selected as the control group. After informed consent, cubital venous blood of all subjects was collected, and epidemiological data of the subjects were collected; the rs2383206, rs2383207, rs10757274, and rs1333049 on chromosome 9p21 as the sites to be tested, using Sequenom Mass Array system for genotyping, using Haploview4.2 software to calculate whether the genotype distribution meets Hardy-Weinberg equilibrium. Results showed that there were no significant differences between the two groups in gender, age, and smoking history. There are significant differences in the levels of hypertension, diabetes and hyperlipidemia, total cholesterol, triglycerides, HDL-C and apolipoprotein A1 between the two groups of study subjects. The genotype frequencies of the participating populations were in a balanced state. The results of the association analysis between SNPs and IS susceptibility showed that rs2383207, rs10757274, rs1333049 and rs2383206 are the susceptibility sites of ischemic stroke. It concluded that in Anhui, China, the inheritance of chromosome 9p21 region is associated with ischemic stroke.

show abstract

Altered Protein and Gene Expression of Beclin-1 Correlates with Poor Prognosis of Hcv-Associated Hepatocellular Carcinoma in Egyptian Patients

Cited by 4 publications

References 22 publications

Effect and mechanism of Angelic Shaoyaosan mediated AMPK/SIRT1 positive feedback loop to promote autophagy and regulate the systemic inflammatory response in acute pancreatitis

Effect and mechanism of Angelic Shaoyaosan mediated AMPK/SIRT1 positive feedback loop to promote autophagy and regulate the systemic inflammatory response in acute pancreatitis

Redox Regulation of Autophagy in Cancer: Mechanism, Prevention and Therapy

Common genetic variants on chromosome 9p21 confers risk of ischemic stroke: A large-scale genetic association study

Contact Info

Product

Resources

About