Altered platelet aggregation and coagulation disorders related to clinical findings in 30 dogs naturally infected by Leishmania infantum

Ciaramella, Paolo; Pelagalli, Alessandra; Cortese, Laura; Pero, Maria Elena; Corona, Mario; Lombardi, Pietro; Avallone, Luigi; Persechino, A.

doi:10.1016/j.tvjl.2004.03.009

Cited by 39 publications

(47 citation statements)

References 11 publications

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“…CVL generally does not cause severe liver injuries 6 because most liver lesions are due to the spread of infected macrophages, thus causing a chronic infection in this organ 24 . In this regard, these results do not corroborate the findings from studies done by Ciaramella et al 25 , which revealed a considerable increase in the concentration of GPT in animals with clinical symptoms.…”

Section: Conflict Of Interest Financial Support Referencescontrasting

confidence: 99%

Clinical and laboratory alterations in dogs naturally infected by Leishmania chagasi

Freitas

Nunes-Pinheiro

Neto

et al. 2012

Rev. Soc. Bras. Med. Trop.

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Introduction: Canine visceral leishmaniasis (CVL) is a zoonotic disease with different clinical manifestations. Parasitism often occurs in bone marrow, but changes have been observed in peripheral blood and serum biochemical parameters. The aim of this study was to evaluate the hematological and biochemical parameters in dogs naturally infected by Leishmania chagasi. Methods: Eighty-five adult dogs of both sexes and various weights and ages from the Zoonosis Control Center of Fortaleza (CCZ) were used, selected by immunofluorescence assay (IFA) and considered positive with IFA titers greater than 1:40 and by visualizing amastigotes of Leishmania chagasi in smears obtained by bone marrow aspiration. The dogs (n = 85) were grouped according to clinical signs: negative (CN = 7), subclinical (CS = 10), and clinical (CC = 68). Blood samples were collected for determination of hematological and biochemical serum values. The experimental protocol was approved by the CEUA/UECE. Results: The most frequent clinical signs were cachexia (77.9%), keratitis (61.8%), and lymphadenopathy (55.9%), and 86.8% of the animals showed more than one clinical sign characteristic of CVL. In CC were observed reductions in red blood cells (63%), hematocrit (72%), and hemoglobin (62%), as well as leukocytosis (33%), neutropenia (28%), thrombocytopenia (50%), uremia (45%), hyperproteinemia (53%, p<0.05), hypergammaglobulinemia (62%, p<0.01), and hypoalbuminemia (58%). Conclusions: Animals with the clinical form of the disease demonstrate hematological and biochemical changes consistent with anemia, uremia, hyperproteinemia, and hyperglobulinemia, which present themselves as strong clinical markers of visceral leishmaniasis associated with the signs previously reported. Keywords: Dogs. Canine visceral leishmanisis. Biomarkers. Anemia. Uremia. Hyperglobulinemia. RESUMO Introdução:A leishmaniose visceral canina (LVC) é uma zoonose com diferentes manifestações clínicas. O parasitismo ocorre frequentemente na medula óssea e têm sido relatadas alterações hematológicas e bioquímicas. Objetivou-se avaliar os parâmetros clínicos, hematológicos e bioquímicos de cães naturalmente infectados por Leishmania chagasi. Métodos: Utilizaram-se 85 cães adultos, ambos os sexos, peso e idade variados, oriundos do Centro de Controle de Zoonoses de Fortaleza, selecionados pela reação de imunofluorescência indireta (RIFI), sendo considerados positivos os animais com títulos de RIFI ≥ 1:40 e pelo exame parasitológico das formas amastigotas de Leishmania chagasi em esfregaços de medula óssea. Os cães foram agrupados conforme os sinais clínicos associados à doença: negativos (CN=7); subclínicos (CS=10) e clínicos (CC=68). Amostras de sangue foram coletadas para determinação dos parâmetros hematológicos e bioquímicos séricos. O protocolo experimental foi aprovado pelo CEUA/UECE, protocolo n° 08622833-1. Resultados: Os sinais clínicos mais frequentes foram caquexia (77,9%), ceratoconjuntivite (61,8%) e linfadenopatia (55,9%), sendo que 86, 8% dos animais aprese...

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Section: Conflict Of Interest Financial Support Referencescontrasting

confidence: 99%

Clinical and laboratory alterations in dogs naturally infected by Leishmania chagasi

Freitas

Nunes-Pinheiro

Neto

et al. 2012

Rev. Soc. Bras. Med. Trop.

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“…The absence of hemorrhagic diathesis observed in dogs with leishmaniasis contradicts previous findings reported in coagulation studies with similar number of dogs evaluated (MORENO, 1999;CIARAMELLA et al, 2005). This difference may be related to the disease clinical evolution, which depends not only on the presence of the protozoan, but also of the inflammatory process, compromised immunological response and renal and hepatic failure occurring in markedly symptomatic dogs (CIARAMELLA et al, 2005).…”

Section: Introductionmentioning

confidence: 40%

“…Canine visceral leishmaniasis (CVL) is a multisystemic disease which infects humans, wild animals and domestic dogs (CIARAMELLA et al, 2005;SARIDOMICHELAKIS, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…Hemostatic abnormalities may cause thrombocytopenia, prolonged prothrombin time (PT) and activated partial thromboplastin time (APTT), increased fibrinogen and fibrin degradation products occur frequently (MORENO, 1999;SOLANO-GALLEGO et al, 2001;CIARAMELLA et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…This difference may be related to the disease clinical evolution, which depends not only on the presence of the protozoan, but also of the inflammatory process, compromised immunological response and renal and hepatic failure occurring in markedly symptomatic dogs (CIARAMELLA et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Hemostatic assessment of dogs associated with hepatic parasite load of Leishmania infantum chagasi

Torres

Almeida

Paula

et al. 2016

Rev. Bras. Parasitol. Vet.

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Leishmania infantum chagasi liver parasite load was compared to hemostatic abnormalities, as well as to clinical, laboratorial, and histopathological findings in dogs with visceral leishmaniasis. The liver parasite load of 30 dogs L. infantum chagasi naturally-infected was evaluated by quantitative real-time PCR and the results were compared with serum biochemistry and primary and secondary hemostasis findings. Moreover, hepatic histological lesions were described in these dogs. Prolonged bleeding time, prothrombin time (PT), and activated partial thromboplastin time (APTT), were observed in the group with visceral leishmaniasis. Eleven dogs presented inflammatory liver lesions, with predominance of mild multifocal mononuclear periportal hepatitis. No association between the presence of parasites and abnormalities in screening tests was observed by Spearman's rank correlation coefficient. The clinical progression in leishmaniasis is associated with the occurrence of hemorrhagic diathesis, which depends not only on the presence of the parasite but also the inflammatory process, compromised immunological response, hepatic and renal failure in symptomatic dogs.Keywords: Coagulation, leishmaniasis, liver, qPCR. ResumoA carga parasitária de Leishmania infantum chagasi do fígado foi comparada às anormalidades hemostáticas, bem como aos achados clínicos, laboratoriais e histopatológicos em cães com leishmaniose visceral. A carga parasitária do fígado de 30 cães naturalmente infectados por L. infantum chagasi foi avaliada por PCR quantitativo em tempo real e os resultados foram comparados com bioquímica sérica e achados de hemostasia primária e secundária. Além disso, foram descritas as lesões hepáticas nestes cães. Prolongado tempo de sangramento, tempo de protrombina (TP) e tempo de tromboplastina parcial ativada (TTPA) foram observados no grupo com leishmaniose visceral. Onze cães apresentaram lesões inflamatórias no fígado, predominando hepatite periportal mononuclear multifocal. Não foi observada associação entre a presença de parasitos e as anormalidades nos testes laboratoriais por correlação de Spearman. A progressão clínica na leishmaniose está associada com a ocorrência de diátese hemorrágica, que depende não só da presença do parasito, mas também do processo inflamatório, do comprometimento da resposta imunológica e da falência renal e hepática em cães sintomáticos.Palavras-chave: Coagulação, leishmaniose, fígado, qPCR.

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Leishmaniosis

Baneth¹,

Petersen²,

Solano‐Gallego³

et al. 2021

Greene's Infectious Diseases of the Dog and Cat

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Altered platelet aggregation and coagulation disorders related to clinical findings in 30 dogs naturally infected by Leishmania infantum

Cited by 39 publications

References 11 publications

Clinical and laboratory alterations in dogs naturally infected by Leishmania chagasi

Clinical and laboratory alterations in dogs naturally infected by Leishmania chagasi

Hemostatic assessment of dogs associated with hepatic parasite load of Leishmania infantum chagasi

Leishmaniosis

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