Altered neurotransmitter expression profile in the ganglionic bowel in Hirschsprung's disease

Coyle, David; O’Donnell, Anne Marie; Gillick, John; Puri, Prem

doi:10.1016/j.jpedsurg.2016.02.018

Cited by 21 publications

(18 citation statements)

References 24 publications

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“…These neurotransmitter changes were also confirmed in HSCR animal models [14,15]. Our study suggested that the acetylcholine content in the colon of HSCR mice varied in the different location of colon.…”

Section: Discussionsupporting

confidence: 78%

The role of the α7nAChR-mediated cholinergic anti-inflammatory pathway in Hirschsprung associated enterocolitis

Chen

Wei

Chen

et al. 2020

Preprint

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Background To investigate the role and the underlying mechanism of the α7nAChR-mediated cholinergic anti-inflammatory pathway in the pathogenesis of Hirschsprung(HSCR) associated enterocolitis（HAEC）. Methods Experimental group:twenty-one-day-old Ednrb-/- mice were selected (n=10), with comparable-age wild type(Ednrb+/+) mice controls (n=10). Intestinal samples were collected. The experimental colons were divided into narrow and dilated segments according to morphology changes. The control colons were divided into distal and proximal segments.Colon HE staining was used to judge HAEC.Acetylcholine levels in colon was measured using enzyme-linked immunosorbent assays. Detected phosphorylated Jak2 (p-Jak2), Jak2, phosphorylated Stat3 (p-Stat3), Stat3, phosphorylated IκBα (p-IκBα) and IκBα were studied by Western blotting; mRNA levels of Jak2, Stat3, and IκBα were detected by RT-qPCR. Results Colon HE staining indicated that HAEC mainly occured in the dilated segments of HSCR mice (Ednrb-/- mice) (EDNRB-P).Acetylcholine content in EDNRB-P was significantly lower than that in the narrow segments (EDNRB-D) (P<0.05). Western blotting showed that the Jak2, p-Jak2, Stat3 and p-Stat3 levels in EDNRB-D were significantly higher than those in EDNRB-P (P<0.05). The p-IκBα and IκBα levels in EDNRB-P were significantly higher than those in EDNRB-D(P<0.05). The mRNA levels of Jak2 and Stat3 in EDNRB-D were higher than those in EDNRB-P, but the IκBα mRNA level was significantly lower than that in EDNRB-P (P<0.05). Conclusions During HAEC, the inflammation in the dilated segment was more severe ,while in the narrow segment there was no obvious inflammatory reaction and the content of acetylcholine was higher, which was associated with the α7nAChR-mediated cholinergic anti-inflammatory pathway.

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Section: Discussionsupporting

confidence: 78%

The role of the α7nAChR-mediated cholinergic anti-inflammatory pathway in Hirschsprung associated enterocolitis

Chen

Wei

Chen

et al. 2020

Preprint

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“…Forty-three (8.4 %) patients with HD in the present meta-analysis required further secondary procedures after the first pull-through, reflecting the complexity of surgery for this disease. Recent studies suggest that the ganglionic bowel in patients with HD has abnormalities in innervation, further complicating the management of HD [23,24,[27][28][29][30]. However, only a very few complications due to laparoscopy such as perforation of the colon, division of the ureter or bladder perforation occurred, which makes the laparoscopic approach a safe addendum in the pull-through procedure.…”

Section: Discussionmentioning

confidence: 99%

Laparoscopic-assisted pull-through operation for Hirschsprung’s disease: a systematic review and meta-analysis

2016

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This meta-analysis shows that nearly one-third of the patients continue to have long-term bowel problems, such as constipation, soiling and recurrent enterocolitis following LAPT. Many patients treated by LAPT require secondary surgery. Large randomized studies with long-term follow-up are necessary to determine the difference in outcome between LAPT and completely transanal pull-through operation.

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“…ВИП-положительные антитела были обнаружены как в межмышечном, так и в подслизистом нервных сплетениях кишечника. Отмечено, что особенно многочисленны ВИП-положительные нервные волокна в циркулярном мышечном слое здорового участка кишки при БГ, в то время как в аганглионарном сегменте ВИПклетки не определялись [42].…”

Section: особенности течения болезни гиршпрунга у детей с синдромом дunclassified

Hirschsprung’s Disease in Children with Down Syndrome. Features of Clinical Aspects, Diagnostics and Treatment

Морозов¹,

Pimenova²,

Марчук³

2020

Novosti Khirurgii

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Altered neurotransmitter expression profile in the ganglionic bowel in Hirschsprung's disease

Cited by 21 publications

References 24 publications

The role of the α7nAChR-mediated cholinergic anti-inflammatory pathway in Hirschsprung associated enterocolitis

The role of the α7nAChR-mediated cholinergic anti-inflammatory pathway in Hirschsprung associated enterocolitis

Laparoscopic-assisted pull-through operation for Hirschsprung’s disease: a systematic review and meta-analysis

Hirschsprung’s Disease in Children with Down Syndrome. Features of Clinical Aspects, Diagnostics and Treatment

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