Altered mucosal expression of microRNAs in pediatric patients with inflammatory bowel disease

Béres, Nóra; Kiss, Zoltán; Sztupinszki, Zsófia; Lendvai, Gábor; Arató, András; Sziksz, Erna; Vannay, Ádám; Szabó, Attila; Müller, Katalin Eszter; Aron, C; Boros, Kriszta Katinka; Veres, Gábor

doi:10.1016/j.dld.2016.12.022

Cited by 30 publications

(26 citation statements)

References 61 publications

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“…Statistical significance was defined as p < 0.05. AU = arbitrary units (which represent the expression of each miR normalized toU6sn RNA internal control), miR = microRNA data supporting its usefulness as a CD specific diagnostic marker, both in adults and children [62,75]. In contrast to human studies, we found decreased relative expression in the colonic mucosa of dogs with IBD compared to healthy controls.…”

Section: Discussionmentioning

confidence: 49%

Colonic mucosal and serum expression of microRNAs in canine large intestinal inflammatory bowel disease

et al. 2020

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Background: Canine inflammatory bowel disease (IBD) is a group of chronic gastrointestinal (GI) disorders of still largely unknown etiology. Canine IBD diagnosis is time-consuming and costly as other diseases with similar signs should be initially excluded. In human IBD microRNA (miR) expression changes have been reported in GI mucosa and blood. Thus, there is a possibility that miRs may provide insight into disease pathogenesis, diagnosis and even treatment of canine IBD. The aim of this study was to determine the colonic mucosal and serum relative expression of a miRs panel in dogs with large intestinal IBD and healthy control dogs. Results: Compared to healthy control dogs, dogs with large intestinal IBD showed significantly increased relative expression of miR-16, miR-21, miR-122 and miR-147 in the colonic mucosa and serum, while the relative expression of miR-185, miR-192 and miR-223 was significantly decreased. Relative expression of miR-146a was significantly increased only in the serum of dogs with large intestinal IBD. Furthermore, serum miR-192 and miR-223 relative expression correlated to disease activity and endoscopic score, respectively. Conclusion: Our data suggest the existence of dysregulated miRs expression patterns in canine IBD and support the potential future use of serum miRs as useful noninvasive biomarkers.

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Section: Discussionmentioning

confidence: 49%

Colonic mucosal and serum expression of microRNAs in canine large intestinal inflammatory bowel disease

et al. 2020

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“…We and others have identified miR-31 as a discriminant more generally of CD and NIBD patients (11,12). We hypothesized that this difference is driven primarily by CD patients in the IL group.…”

Section: Resultsmentioning

confidence: 88%

Colonic epithelial miR-31 associates with the development of Crohn’s phenotypes

Keith¹,

Barrow²,

Toyonaga³

et al. 2018

JCI Insight

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BACKGROUND. Crohn's disease (CD) is highly heterogeneous, due in large part to variability in cellular processes that underlie the natural history of CD, thereby confounding effective therapy. There is a critical need to advance understanding of the cellular mechanisms that drive CD heterogeneity. METHODS. We performed small RNA sequencing of adult colon tissue from CD and NIBD controls. Colonic epithelial cells and immune cells were isolated from colonic tissues, and microRNA-31 (miR-31) expression was measured. miR-31 expression was measured in colonoid cultures generated from controls and patients with CD. We performed small RNA-sequencing of formalinfixed paraffin-embedded colon and ileum biopsies from treatment-naive pediatric patients with CD and controls and collected data on disease features and outcomes. RESULTS. Small RNA-sequencing and microRNA profiling in the colon revealed 2 distinct molecular subtypes, each with different clinical associations. Notably, we found that miR-31 expression was a driver of these 2 subtypes and, further, that miR-31 expression was particularly pronounced in epithelial cells. Colonoids revealed that miR-31 expression differences are preserved in this ex vivo system. In adult patients, low colonic miR-31 expression levels at the time of surgery were associated with worse disease outcome as measured by need for an end ileostomy and recurrence of disease in the neoterminal ileum. In pediatric patients, lower miR-31 expression at the time of diagnosis was associated with future development of fibrostenotic ileal CD requiring surgery CONCLUSIONS. These findings represent an important step forward in designing more effective clinical trials and developing personalized CD therapies.

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“…In a study on patients with Crohn's disease, miRNA-204 expressions were found to be increased in the affected intestinal mucosa. 18 Conversely, Lai et al 19 have found that the plasma miRNA-204 levels were lower in patients with RA and it was increased after anti-TNF-alpha treatment. In a current research, Koga et al 20 have demonstrated that miRNA-204 is significantly decreased in plasma of FMF patients.…”

Section: Discussionmentioning

confidence: 99%

“…6 Serum miRNA-204 levels were commonly studied in different types of cancer, rheumatoid arthritis (RA) and various inflammatory diseases, with significant alterations detected in plasma expression. [14][15][16][17][18][19] Moreover, the miRNA-204 was demonstrated to have suppressor effects on inflammatory cytokine production by targeting the phosphoinositide 3-kinase gamma (PI3Kg) pathway and that plasma miR-204 level can be used as a potential biomarker in patients with FMF. 20 Rosenberger et al 21 have shown that miRNA-451 has a role in cytokine production in dendritic cells.…”

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confidence: 99%

Assessment of Circulating Microribonucleic Acids in Patients With Familial Mediterranean Fever

2020

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Objectives: This study aims to evaluate the plasma expression of microribonucleic acids (miRNAs) that may be associated with the pathogenesis of familial Mediterranean fever (FMF). Patients and methods: Thirty patients with FMF (18 males, 12 females; mean age 9.1±4.7 years; range, 3 to 15.5 years) and 30 age-and sex-matched healthy children (18 males, 12 females; mean age 9.5±4.6 years; range, 4 to 16.5 years) were included in this study. The plasma levels of four candidate miRNAs (miRNA-16, miRNA-155, miRNA-204 and miRNA-451) were measured in all subjects. The plasma levels of miRNAs were analyzed with real-time polymerase chain reaction in attack and remission periods of patients and healthy controls (HCs). Results: Plasma miRNA-204 levels of FMF patients were decreased 6.5 fold in remission period compared to HCs (p<0.001). This decrease was more prominent in M694V mutation carriers. Plasma miRNA-155 levels of FMF patients were lower in remission period (p=0.03). Conclusion: Our findings showed significant alterations in the plasma expression of miRNA-155 and miRNA-204 in FMF patients compared to HCs. Our data suggest that miRNA-155 and miRNA-204 may be related to the pathogenesis of FMF. Further comprehensive and functional researches may help to clarify the role of miRNAs in FMF and elucidate the pathogenesis of the disease.

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Altered mucosal expression of microRNAs in pediatric patients with inflammatory bowel disease

Cited by 30 publications

References 61 publications

Colonic mucosal and serum expression of microRNAs in canine large intestinal inflammatory bowel disease

Colonic mucosal and serum expression of microRNAs in canine large intestinal inflammatory bowel disease

Colonic epithelial miR-31 associates with the development of Crohn’s phenotypes

Assessment of Circulating Microribonucleic Acids in Patients With Familial Mediterranean Fever

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