Colonic mucosal and serum expression of microRNAs in canine large intestinal inflammatory bowel disease

Konstantinidis, Antonis; Pardali, Dimitra; Adαmama-Moraitou, K. K.; Gazouli, Maria; Dovas, Chrysostomos Ι.; Legaki, Evangelia; Brellou, Georgia; Savvas, Ioannis; Jergens, Albert E.; Rallis, Timoleon; Allenspach, Karin

doi:10.1186/s12917-020-02287-6

Cited by 18 publications

(26 citation statements)

References 93 publications

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“…Recent studies reported the downregulation of miRNA-223 in IBD (Din et al, 2020, Konstantinidis et al, 2020, Wu et al, 2020). This was consistent with our findings, in which miRNA-223 expression was significantly decreased following AA-induction of UC in rats.…”

Section: Discussionmentioning

confidence: 99%

Protective effect ofMorus macrouraMiq. fruit extract against acetic acid-induced ulcerative colitis in rats: Involvement of miRNA-223 and TNFα/NFκB/NLRP3 inflammatory pathway

Salama

Darwish

Shaffei

et al. 2020

Preprint

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Tumor necrosis factor receptor (TNFR) activation and nuclear factor kappa B (NFκB) expression play a significant role in the activation of nod-like receptor pyrin domain-1 containing 3 (NLRP3) inflammasome inflammatory pathway, which is involved in the pathogenesis of ulcerative colitis (UC). Furthermore, miRNA-223 expression was shown to exert counter-regulatory effect on NLRP3 expression. Interestingly, polyphenols are attaining increased importance for their potential effectiveness in ameliorating certain diseases owing to their antioxidant and anti-inflammatory activity. In accord, our study attempted to investigate the effect of mulberry tree (Morus macroura) fruit extract (MFE) against acetic acid (AA)- induced UC in rats, which is not previously investigated, based on previous promising results for MFE in alleviating gastric ulcer in rats. First, total phenolic (TPC), total flavonoid (TFC), and total anthocyanin content (TAC) were determined in MFE. Then, MFE (300 mg/kg) and sulfasalazine (Sulfa), as a standard treatment (100 mg/kg), were given orally for seven days before intra-rectal induction of UC by AA (2 ml, 4% v/v) on day eight. The extent of UC was evaluated macroscopically and microscopically. Biochemically, the colonic TNFR1, NLRP3, p- NFκB p65, TNFα, interleukin-1 beta (IL-1β), IL-18 levels, miRNA-223 expression and caspase-1 activity were assayed. MFE significantly reduced macroscopic and microscopic scores, colonic levels of TNFR1, NLRP3, p-NFκB p65, TNFα, IL-1β, IL-18, and caspase-1 activity, and showed increased miRNA-223 expression, almost similarly to Sulfa effects. In conclusion, our study provided a novel impact for MFE against AA-induced UC in rats through affecting miRNA-223 expression and halting TNFα/NFκB/NLRP3 inflammatory pathway.

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Section: Discussionmentioning

confidence: 99%

Protective effect ofMorus macrouraMiq. fruit extract against acetic acid-induced ulcerative colitis in rats: Involvement of miRNA-223 and TNFα/NFκB/NLRP3 inflammatory pathway

Salama

Darwish

Shaffei

et al. 2020

Preprint

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“…25 Recently, Konstantinidis et al 26 found significantly increased relative expression of miR-16, miR-21, miR-122 and miR-147 in the colonic mucosa and serum of dogs with large intestinal IBD. 26 miR-16 was also reported to be elevated in peripheral blood of patients with rheumatoid arthritis, pointing to a possible relation of miR-16 in the pathophysiologic mechanism common to autoimmune disorders and IBDs. 25 Not only serum expression of miRNAs that was used to indicate the disease but also tissue miRNA is also used.…”

Section: Discussionmentioning

confidence: 99%

Serum expression of microRNA-16 in a cohort of Egyptian patients with ulcerative colitis and its correlation with disease extent and severity

Saad

Ali

Baddour

et al. 2020

Journal of Coloproctology

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Ulcerative colitis is one of the IBDs. Its etiology and pathogenesis remain undefined with an interaction between environmental, genetic and immunological factors is the most accepted explanation. Several recent studies have examined microRNA expression in the peripheral blood and tissues from IBD patients. The study aims at assessing the expression of serum miR-16 in ulcerative colitis patients and its correlation with disease extent, activity and severity. It included 30 treatment naїve ulcerative colitis patients of different presentations. Serum miR-16 expression was assessed using reverse transcriptase quantitative real time PCR (RT-qPCR), and then correlated with that of a group of 20 healthy subjects to assess its role in diagnosis of ulcerative colitis. Also, it was correlated with disease extent (proctitis, left sided colitis, extensive colitis) and disease activity and severity indices (Truelove and Witts criteria, fecal calprotectin and UCEIS). Thirty ulcerative colitis patients were enrolled, 53% had mild, 37% had moderate, while 10% had severe disease. Concerning endoscopic extent, 8 had proctitis, 14 had left sided colitis and 8 had extensive colitis. Serum expression of miR-16 in the 30 patients were compared to that of the healthy control subjects. The patients’ group showed median serum miR-16 expression of 1.91, 1.13 for the control group with a significant difference between both groups. Correlation between serum miR-16 expression with disease extent, activity and severity showed no significant relation. From the current study we can conclude that increased serum expression of miR-16 is associated with ulcerative colitis despite no significant relation to disease activity extent or severity.

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“…26 dogs presented or referred to Companion Animal Clinic, School of Veterinary Medicine, Aristotle University of Thessaloniki for investigating large intestinal disease and were diagnosed with IBD. The dogs with large intestinal IBD described here were also used in another study [ 30 ]. Selection criteria, as previously described [ 30 ], included (1) a history of chronic (>3 weeks) large intestinal disease (large intestinal diarrhea with mucus, hematochezia, tenesmus, and/or increased frequency of defecation) without any identifiable underlying cause, (2) histopathologic evidence of intestinal inflammatory cellular infiltration and (3) minor or no response to a 3-week dietary trial (novel or hydrolyzed protein diets) to rule out adverse food reactions.…”

Section: Methodsmentioning

confidence: 99%

“…The dogs with large intestinal IBD described here were also used in another study [ 30 ]. Selection criteria, as previously described [ 30 ], included (1) a history of chronic (>3 weeks) large intestinal disease (large intestinal diarrhea with mucus, hematochezia, tenesmus, and/or increased frequency of defecation) without any identifiable underlying cause, (2) histopathologic evidence of intestinal inflammatory cellular infiltration and (3) minor or no response to a 3-week dietary trial (novel or hydrolyzed protein diets) to rule out adverse food reactions. Exclusion criteria were (1) the presence of a concurrent disease and (2) administration of any medication for a 2-week period prior to presentation or referral.…”

Section: Methodsmentioning

confidence: 99%

Colonic mucosal and cytobrush sample cytokine mRNA expression in canine inflammatory bowel disease and their correlation with disease activity, endoscopic and histopathologic score

et al. 2021

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Canine inflammatory bowel disease (IBD) is a group of chronic gastrointestinal disorders, the pathogenesis of which remains elusive, but it possibly involves the interaction of the intestinal immune system with luminal microbiota and food-derived antigens. Mucosal cytokines profiles in canine IBD have been investigated mainly in small intestinal disease, while data on cytokine profiles in large intestinal IBD are limited. The objective of this study was to measure colonic mucosal and cytobrush sample messenger (m)RNA expression of interleukin (IL)-1β, IL-2, IL-12p40, IL-23p19, tumor necrosis factor-alpha (TNF-α) and chemokine C‐C motif ligand (CCL28) in dogs with IBD and healthy controls using quantitative real-time polymerase chain reaction (PCR), and assess their correlation with clinical disease activity, endoscopic and histopathologic score. Dogs with IBD had a significantly increased mRNA expression of IL-1β, IL-23p19 and CCL28 in the colonic mucosa, compared to healthy controls. None of the selected cytokines had significantly different mRNA expression in the colonic cytobrush samples between the two groups or between the colonic mucosa and cytobrush samples of dogs with IBD. Finally, there was a statistically significant correlation of clinical disease activity with endoscopic activity score and fibrosis and atrophy of the colonic mucosa in dogs with large intestinal IBD. IL-1β, IL-23p19 and CCL28 could play a role in the pathogenesis of canine large intestinal IBD. Colonic cytokine expression does not correlate with clinical disease activity and/or endoscopic score. However, clinical signs reflect the severity of endoscopic lesions.

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Colonic mucosal and serum expression of microRNAs in canine large intestinal inflammatory bowel disease

Cited by 18 publications

References 93 publications

Protective effect ofMorus macrouraMiq. fruit extract against acetic acid-induced ulcerative colitis in rats: Involvement of miRNA-223 and TNFα/NFκB/NLRP3 inflammatory pathway

Protective effect ofMorus macrouraMiq. fruit extract against acetic acid-induced ulcerative colitis in rats: Involvement of miRNA-223 and TNFα/NFκB/NLRP3 inflammatory pathway

Serum expression of microRNA-16 in a cohort of Egyptian patients with ulcerative colitis and its correlation with disease extent and severity

Colonic mucosal and cytobrush sample cytokine mRNA expression in canine inflammatory bowel disease and their correlation with disease activity, endoscopic and histopathologic score

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