Altered miRNA expression profiles are involved in the protective effects of troxerutin against ultraviolet B radiation in normal human dermal fibroblasts

Jun, Hwa; Lee, Kwang-Sik; Lee, Ghang Tai; Lee, Kun Kook; Hong, Jin Tae; Lee, Sung Nae; Jang, Hyun Hee; Lee, Jae Ho; Park, In-Chul; Kim, Yu Ri; Ahn, Kyu Joong; Kwon, Seung Bin; An, In‐Sook; An, Sungkwan; Bae, Seunghee

doi:10.3892/ijmm.2014.1647

Cited by 12 publications

(16 citation statements)

References 33 publications

(16 reference statements)

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“…In particular, troxerutin has been observed to exhibit an inhibitory effect on the neurotoxicity induced by high cholesterol mediated cognitive deficits, kainic acid-triggered excitotoxic damage and β-amyloid oligomerization (3–5). In addition, troxerutin has a photoprotective effect against ultraviolet B (UVB) radiation in human skin cells, including dermal fibroblasts and keratinocytes (6,7). Troxerutin also exerts a protective effect against γ-radiation in mice (8,9).…”

Section: Introductionmentioning

confidence: 99%

Analysis of changes in microRNA expression profiles in response to the troxerutin-mediated antioxidant effect in human dermal papilla cells

Lim

Lee

et al. 2015

Molecular Medicine Reports

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Dermal papilla (DP) cells function as important regulators of the hair growth cycle. The loss of these cells is a primary cause of diseases characterized by hair loss, including alopecia, and evidence has revealed significantly increased levels of reactive oxygen species (ROS) in hair tissue and DP cells in the balding population. In the present study, troxerutin, a flavonoid derivative of rutin, was demonstrated to have a protective effect against H2O2-mediated cellular damage in human DP (HDP) cells. Biochemical assays revealed that pretreatment with troxerutin exerted a protective effect against H2O2-induced loss of cell viability and H2O2 induced cell death. Further experiments confirmed that troxerutin inhibited the H2O2-induced production of ROS and upregulation of senescence-associated β-galactosidase activity. Using microRNA (miRNA) microarrays, the present study identified 24 miRNAs, which were differentially expressed in the troxerutin pretreated, H2O2-treated HDP cells. Subsequent prediction using bioinformatics analysis revealed that the altered miRNAs were functionally involved in several cell signaling pathways, including the mitogen-activated protein kinase and WNT pathways. Overall, these results indicated that ROS-mediated cellular damage was inhibited by troxerutin and suggested that the use of troxerutin may be an effective approach in the treatment of alopecia.

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Section: Introductionmentioning

confidence: 99%

Analysis of changes in microRNA expression profiles in response to the troxerutin-mediated antioxidant effect in human dermal papilla cells

Lim

Lee

et al. 2015

Molecular Medicine Reports

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“…Previous reports have demonstrated that cisplatin-and androgen overexposure-mediated cellular dysfunction, including apoptosis, can induce alopecia and occur predominantly by stimulating the production ROS in HDP cells (16,23,24). In our previous study, pretreatment of human dermal fibroblasts and HaCaT keratinocytes with troxerutin was observed to protect against UVB-mediated cell death (6,7). UVB light is an important inducer of ROS production in several types of cell (13); therefore, the present study examined the possible role of troxerutin in protecting against ROS-induced cell stress and damage in HDP cells.…”

Section: H 2 O 2 -Induced Cell Damage Is Inhibited By Troxerutin In Hmentioning

confidence: 85%

“…Oxidative stress stimulates the production of a known inhibitor of hair follicles, tumor growth factor-β, in DPC cells, which induces the onset of androgenic alopecia (24). Our previous studies demonstrated that troxerutin has a photoprotective effect against UV radiation on dermal fibroblasts and keratinocytes (6,7), and several clinical and theoretical reports have revealed that UV radiation has negative effects on hair growth through the induction of oxidative stress, acute telogen effluvium and follicular micro-inflammation in follicular stem cells (31)(32)(33). Therefore, countering oxidative stress can be considered an important strategy to overcome stress-or androgen-dependent alopecia, and the results of the present study confirmed that troxerutin inhibited oxidative stress-induced cellular damage in the DPC cells.…”

Section: Discussionmentioning

confidence: 99%

“…In particular, troxerutin has been observed to exhibit an inhibitory effect on the neurotoxicity induced by high cholesterol-mediated cognitive deficits, kainic acid-triggered excitotoxic damage and β-amyloid oligomerization (3)(4)(5). In addition, troxerutin has a photoprotective effect against ultraviolet B (UVB) radiation in human skin cells, including dermal fibroblasts and keratinocytes (6,7). Troxerutin also exerts a protective effect against γ-radiation in mice (8,9).…”

Section: Introductionmentioning

confidence: 99%

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Analysis of changes in microRNA expression profiles in response to the troxerutin-mediated antioxidant effect in human dermal papilla cells

Lee¹

2016

Clin Exp Dermatol Res

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Abstract. Dermal papilla (DP) cells function as important regulators of the hair growth cycle. The loss of these cells is a primary cause of diseases characterized by hair loss, including alopecia, and evidence has revealed significantly increased levels of reactive oxygen species (ROS) in hair tissue and DP cells in the balding population. In the present study, troxerutin, a flavonoid derivative of rutin, was demonstrated to have a protective effect against H 2 O 2 -mediated cellular damage in human DP (HDP) cells. Biochemical assays revealed that pretreatment with troxerutin exerted a protective effect against H 2 O 2 -induced loss of cell viability and H 2 O 2 -induced cell death. Further experiments confirmed that troxerutin inhibited the H 2 O 2 -induced production of ROS and upregulation of senescence-associated β-galactosidase activity. Using microRNA (miRNA) microarrays, the present study identified 24 miRNAs, which were differentially expressed in the troxerutin-pretreated, H 2 O 2 -treated HDP cells. Subsequent prediction using bioinformatics analysis revealed that the altered miRNAs were functionally involved in several cell signaling pathways, including the mitogen-activated protein kinase and WNT pathways. Overall, these results indicated that ROS-mediated cellular damage was inhibited by troxerutin and suggested that the use of troxerutin may be an effective approach in the treatment of alopecia.

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“…1 and 2). In the same condition, we analyzed miRNA profile, since, in recent study, HDP cells regulate cellular signal pathway, such as survival, death, and cell cycle arrest (Kim et al 2014;Cha et al 2014). As shown in Figs.…”

Section: Discussionmentioning

confidence: 99%

Epigallocatechin-3-gallate inhibits paclitaxel-induced apoptosis through the alteration of microRNA expression in human dermal papilla cells

Shin

Jun

Kim³

et al. 2018

biomed dermatol

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Background: Paclitaxel well known as anti-cancer drug has been shown to cause alopecia in chemotherapy. The paclitaxel chemotherapy-mediated alopecia is induced by apoptotic damage in human dermal papilla (HDP) cells. Epigallocatechin-3-gallate (EGCG) inhibits apoptosis against anti-cancer drug such as cisplatin. EGCG, one of the green tea extract ingredients, has been reported to enhance cell viability and to inhibit apoptosis. However, it is unclear that EGCG enhances cell viability and inhibits apoptosis against paclitaxel-induced apoptotic damage in HDP cells. Methods: We show cell viability, cell cycle, and microRNA (miRNA) expression in EGCG-mediated rescue cell to paclitaxel-mediated cell death and growth arrest. Results: EGCG promotes cell survival and cell death inhibitory effects and alteration of miRNA expression in paclitaxelexposed HDP cells were investigated. Firstly, paclitaxel increases apoptosis and EGCG promotes cell survival and represses paclitaxel-induced apoptosis in a dose-dependent manner. Fluorescence-activated cell sorting (FACS) analysis showed that EGCG protects apoptosis in paclitaxel-exposed HDP cells. miRNA microarray analysis was performed and 48 miRNAs changed by EGCG in paclitaxel-exposed HDP cells were identified. In gene ontology analysis in silico, miRNAs regulate apoptosis and cell proliferation-regulated genes, such as BCL2L1, BCL2L2, BBC3, and MDM2. In Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, miRNAs are related to mitogen-activated protein kinase (MAPK) signaling pathway and Wnt signaling pathway, which regulate apoptosis and cell proliferation. Conclusions: EGCG inhibits apoptosis through regulating miRNA expression related to apoptosis and cell proliferation in paclitaxel-treated HDP cells.

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Altered miRNA expression profiles are involved in the protective effects of troxerutin against ultraviolet B radiation in normal human dermal fibroblasts

Cited by 12 publications

References 33 publications

Analysis of changes in microRNA expression profiles in response to the troxerutin-mediated antioxidant effect in human dermal papilla cells

Analysis of changes in microRNA expression profiles in response to the troxerutin-mediated antioxidant effect in human dermal papilla cells

Analysis of changes in microRNA expression profiles in response to the troxerutin-mediated antioxidant effect in human dermal papilla cells

Epigallocatechin-3-gallate inhibits paclitaxel-induced apoptosis through the alteration of microRNA expression in human dermal papilla cells

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