Epigallocatechin-3-gallate inhibits paclitaxel-induced apoptosis through the alteration of microRNA expression in human dermal papilla cells

Abstract

Background: Paclitaxel well known as anti-cancer drug has been shown to cause alopecia in chemotherapy. The paclitaxel chemotherapy-mediated alopecia is induced by apoptotic damage in human dermal papilla (HDP) cells. Epigallocatechin-3-gallate (EGCG) inhibits apoptosis against anti-cancer drug such as cisplatin. EGCG, one of the green tea extract ingredients, has been reported to enhance cell viability and to inhibit apoptosis. However, it is unclear that EGCG enhances cell viability and inhibits apoptosis against paclitaxel-induced apoptotic damage in HDP cells. Methods: We show cell viability, cell cycle, and microRNA (miRNA) expression in EGCG-mediated rescue cell to paclitaxel-mediated cell death and growth arrest. Results: EGCG promotes cell survival and cell death inhibitory effects and alteration of miRNA expression in paclitaxelexposed HDP cells were investigated. Firstly, paclitaxel increases apoptosis and EGCG promotes cell survival and represses paclitaxel-induced apoptosis in a dose-dependent manner. Fluorescence-activated cell sorting (FACS) analysis showed that EGCG protects apoptosis in paclitaxel-exposed HDP cells. miRNA microarray analysis was performed and 48 miRNAs changed by EGCG in paclitaxel-exposed HDP cells were identified. In gene ontology analysis in silico, miRNAs regulate apoptosis and cell proliferation-regulated genes, such as BCL2L1, BCL2L2, BBC3, and MDM2. In Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, miRNAs are related to mitogen-activated protein kinase (MAPK) signaling pathway and Wnt signaling pathway, which regulate apoptosis and cell proliferation. Conclusions: EGCG inhibits apoptosis through regulating miRNA expression related to apoptosis and cell proliferation in paclitaxel-treated HDP cells.

Colicin N Mediates Apoptosis and Suppresses Integrin-Modulated Survival in Human Lung Cancer Cells

“…Due to its high proliferative rate being similar to cancer cells, the hair follicle is an organ negatively affected by various chemotherapies [53]. Most of the recommended anticancer drugs for lung cancer significantly induced apoptosis in dermal papilla cells consequently causing drug induced-alopecia and low quality of life in cancer patients [38][39][40]. The absence of apoptosis in human dermal papilla DPCs cells treated with colicin N strongly supports the development of colicin N as a selective anticancer drug for treatment of lung cancer.…”

“…Hair loss is one of major side effects that lessen quality of life in lung cancer patients administered with current standard chemotherapy [35][36][37]. Apoptosis in dermal papilla cells caused by anticancer drugs leads to chemotherapy-induced alopecia [38][39][40]. Therefore, the toxic profile of colicin N in normal cells was investigated in human dermal papilla DPCs cells.…”

Resurfacing receptor binding domain of Colicin N to enhance its cytotoxic effect on human lung cancer cells

“…Inducing cell death in dermal papilla cells coincides with a common side effect of current chemotherapeutic drugs, namely hair loss, which can cause psychological distress in lung cancer patients [40] , [41] , [42] , [43] , the safety profiles of ColN WT and ColN +12 were evaluated in human dermal papilla DPCs cells. The cultures were treated with 0–10 μM of ColN WT and ColN +12 .…”

Innovative Strategies for Hair Regrowth and Skin Visualization

“…In addition, EGCG is used for the treatment of hair loss as a reductase inhibitor [109]. Cellular studies have shown that EGCG increases cell proliferation and decreases cell death by altering the miRNA profiles in HDP cells and sequentially affects paclitaxel-mediated hair loss [112]. In vitro experiments indicated that EGCG promotes hair growth by upregulating phosphorylated Erk and Akt and by increasing the Bcl-2/Bax ratio [108].…”