Altered miR‐155 Expression in Allergic Asthmatic Airways

Malmhäll, Carina; Johansson, Karin; Winkler, Carla; Alawieh, Sahar; Ekerljung, Linda; Rådinger, Madeleine

doi:10.1111/sji.12535

Cited by 36 publications

(32 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 40 41 42 Up to now, the most frequently studied miR in the regulation of asthma molecular and cellular processes is miR-155, which is found to be up-regulated in asthma. 39 43 Previous studies have demonstrated that miR-155 participates in allergic airway inflammation through regulating the transcription factor PU.1. 44 In vivo experiments indicated that miR-155 deficiency contributes to allergic airway inflammation improvement by modulating Th2 responses and ATP-/P2R-induced activation of dendritic cells in mice.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of miR-155-5p Inhibits the Proliferation and Migration of IL-13-Induced Human Bronchial Smooth Muscle Cells by Suppressing TGF-β-Activated Kinase 1/MAP3K7-Binding Protein 2

Shi

Cao

et al. 2018

Allergy Asthma Immunol Res

View full text Add to dashboard Cite

PurposeMolecular mechanisms leading to asthma is still ill-defined. Though the function of microRNAs (miRNAs) in asthma was previously reported, the involvement of miR-155 in important features of this disease remains unknown. The present study was designed to uncover the probable involvement of miR-155-5p in the proliferation and migration of IL-13-induced human bronchial smooth muscle cells (BSMCs) and the intrinsic regulatory mechanism.MethodsThe effects of different concentrations of IL-13 on the proliferation and migration of BSMCs as well as the expression of miR-155-5p and its predicted target transforming growth factor (TGF)-β-activated kinase 1/MAP3K7-binding protein 2 (TAB2) were investigated. The effects of miR-155-5p on the proliferation and migration of interleukin (IL)-13-induced BSMCs was determined in vitro using BSMCs transfected with miR-155 mimic/inhibitor and induced by a high concentration of IL-13. The quantitative real-time polymerase chain reaction (qRTPCR) was employed for determining the expression of miR-155-5p and TAB2. Western blotting was applied to analyze the expression of TAB2 at the protein level. Cell proliferation and migration were assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Transwell assays, respectively.ResultsThe proliferation and migration of BSMCs were dose-dependently increased with IL-13 treatment. Contrariwise, IL-13 dose-dependently inhibited the expression of miR-155-5p in BSMCs. Mechanistic studies showed that inhibition of miR-155-5p further promoted the stimulatory effects of IL-13, whereas overexpression of miR-155 significantly inhibited these effects. In silico studies and luciferase reporter assays indicated that TAB2 was a negatively regulated miR-155-5p target.ConclusionsThese results suggested that miR-155-5p-inhibit the IL-13-induced proliferation and migration of BSMCs by targeting TAB2 and that the IL-13/miR-155/TAB2 pathway could serve as a therapeutic target for pulmonary diseases, especially asthma.

show abstract

Section: Discussionmentioning

confidence: 99%

Overexpression of miR-155-5p Inhibits the Proliferation and Migration of IL-13-Induced Human Bronchial Smooth Muscle Cells by Suppressing TGF-β-Activated Kinase 1/MAP3K7-Binding Protein 2

Shi

Cao

et al. 2018

Allergy Asthma Immunol Res

View full text Add to dashboard Cite

show abstract

“…Patients with atopic dermatitis show, among others, an elevated expression of miR-146a, miR-10b, miR-10a, miR-10a*, MiR216, miR-291-1*, mir-454, miR-29b-1* and down regulation of miR-99a*, miR-34a*. miR-34c-5p and miR-30a [8,9].…”

Section: F) Mirnas and Allergymentioning

confidence: 99%

Bio Immune(G)ene Medicine and the Use of miRNAs to Regulate the Cell

2018

ABBSR

View full text Add to dashboard Cite

MicroRNAs (miRNAs or miRs) are a type of non-coding RNA molecules that regulate the gene expression in a negative way, by downregulating the gene expression mainly at the post-transcriptional level, either by the mRNA degradation process or the inhibition of the translation. The role that many miRNAs play in the pathogenesis of several diseases is well known, such as in the inflammation process, in several steps of the oncogenesis or the metabolism of several virus and bacteria among many others. One of the main limitations in the therapeutic use of miRNAs is the ability to reach the target, as well as doing so without causing any collateral damage. One microRNA can indeed regulate up to 200 target-genes, and one gene can be influenced by a lot of different microRNAs. This is the purpose of the Bio Immune(G)ene Medicine: to achieve the cell without harm, use all the molecular resources available, especially epigenetic with the microRNAs, and to restore the cell homeostasis. The Bio Immune(G)ene Medicine only seeks to play a regulatory biomimetic role, to give the cell the needed information for its own right regulation. Our experience in cell regulation for the past few years has shown the way to fight, for instance, against the deleterious effects of viruses or bacteria in the lymphocytes, also at the background of many autoimmune or allergic diseases, as well as to regulate many other pathological processes. To fulfil this purpose, nanobiotechnology is used to reach the targets; we thus introduce very low doses of miRNAs in nano compounds with the aim to promote the regulation of the main signalling pathways disturbed in a given pathology.

show abstract

“…Human airway smooth muscle cells from asthmatic patients were shown to present elevated levels of microRNA-155 compared to controls (52). Conversely, some studies have shown that microRNA-155 presents lower levels in breath condensates, plasma, nasal mucosa, epithelial cells and sputum of asthmatic patients (50,(52)(53)(54)(55). Others have shown that severe asthma patients present elevated plasma microRNA-155 when compared to mild-moderate asthma patients and to healthy controls.…”

Section: Asthmamentioning

confidence: 99%

MicroRNA-155 Implication in M1 Polarization and the Impact in Inflammatory Diseases

et al. 2020

View full text Add to dashboard Cite

Macrophages are known to have an impact in cytokine signaling in the myriad of organs in which they reside and are classically known to be either pro-inflammatory (M1), anti-inflammatory (M2). Different classes of signaling molecules influence these states, of which, microRNAs represent key modulators. These are short RNA species approximately 21 to 23 nucleotides long that generally act by binding to the 3 untranslated region of mRNAs, regulating their translation, and, thus, the quantity of protein they encode. From these species, microRNA-155 was observed to be of great importance for M1 polarization. Because of it's major implication in M1 polarization microRNA-155 was shown to be implicated in different inflammatory diseases. To name a few, microRNA-155 was shown to be modified in patients with asthma and to correlate with asthma symptoms in mouse model; it has been shown to modulate the activity of foam cells and influence the dimensions of the atherosclerotic plaque and it has also been shown to be of crucial influence in transducing the signal of LPS in septic shock. Because of this, the current review aims to offer an overview of the role of microRNA-155 in M1 polarization, the implication that this poses for the pathophysiology of inflammatory diseases and the potential therapeutic possibilities that this knowledge might bring. Currently, microRNA-155 has been used in clinical trials as a marker of inflammation, but the question remains if it's inhibition will be useful in inflammatory diseases, as other products might have a better cost/benefit ratio.

show abstract

Altered miR‐155 Expression in Allergic Asthmatic Airways

Cited by 36 publications

References 34 publications

Overexpression of miR-155-5p Inhibits the Proliferation and Migration of IL-13-Induced Human Bronchial Smooth Muscle Cells by Suppressing TGF-β-Activated Kinase 1/MAP3K7-Binding Protein 2

Overexpression of miR-155-5p Inhibits the Proliferation and Migration of IL-13-Induced Human Bronchial Smooth Muscle Cells by Suppressing TGF-β-Activated Kinase 1/MAP3K7-Binding Protein 2

Bio Immune(G)ene Medicine and the Use of miRNAs to Regulate the Cell

MicroRNA-155 Implication in M1 Polarization and the Impact in Inflammatory Diseases

Contact Info

Product

Resources

About