Altered Maternal Serum Dynamic Thiol-Disulfide Interchange Reactions in Pregnant Women with Gestational Diabetes Mellitus

Abstract

OBJECTIVE: We aimed to evaluate the alteration of dynamic thiol/disulfide homeostasis which basically shows the oxidative stress in body in pregnant women with gestational diabetes mellitus (GDM) by using a newly developed and automated analysis method. STUDY DESIGN: Study population consisted of body mass index and gestational age-matched pregnant women. Patients were subdivided into 3 groups based on their response to glucose challenge test (GCT) and oral glucose tolerance test (OGTT) results: (1) control group (normal GCT test results n= 87); (2) Impaired glucose tolerance (IGT) group (n= 37); (3) GDM group (n=25). Maternal serum thiol and disulphide levels were compared among three groups. RESULTS: Maternal serum thiol values were found decreased and maternal serum disulphide levels were increased in GDM groups were compared to control and IGT groups (Thiols for controls: 398.19±30.49 µmol/L, for IGT group 395.92±35.52 µmol/L, and for GDM group 371.89±41.14 µmol/L p= 0.002). Disulphides for controls; 17.47±4.38 µmol/L, for IGT; 19.27±3.34 µmol/L, for GDM group; 25.46±4.21 µmol/L, (p=0.001). Disulphide/thiol ratio was found to be increased in GDM group comparing to other groups (Disulphide/thiol ratio: 0.044±0.012 for controls, 0.049±0.009 for IGT group, 0.068±0.0103 for GDM group p=0.001) CONCLUSION: Thiol-disulphide balance has shifted to the oxidative side in pregnant women with GDM. So blood glucose regulation is extremely crucial for reducing the oxidative stress which may lead to damages to vital organs of a mother or possibly to development of a fetus in women with GDM.

Thiol-disulfide homeostasis: an integrated approach with biochemical and clinical aspects

“…There are several studies investigating association between TDH and gestational diabetes mellitus [94][95][96][97]. It has been demonstrated that NT and TT levels were found to be lower than control subjects and decreased NT levels have an increased risk of possible adverse perinatal outcomes [95].…”

“…It has been demonstrated that NT and TT levels were found to be lower than control subjects and decreased NT levels have an increased risk of possible adverse perinatal outcomes [95]. It has been demonstrated that thiol levels were significantly lower whereas disulfide levels were significantly higher in patients with gestational diabetes mellitus compared with both pregnant women with impaired glucose tolerance and uncomplicated pregnant subjects [96]. Authors have investigated pregnant women with gestational diabetes mellitus, obese pregnant women and healthy pregnant women in terms of TDH of cord blood.…”

Novel disulfidptosis‐derived gene blueprint stratifying patients with breast cancer

“…Lower levels of native thiol and total thiol were observed in patients with central serous chorioretinopathy and macular degeneration, as compared to control subjects 30,31 . Study showed that patients with gestational diabetes mellitus had significantly lower levels of thiol, and higher levels of disulfide, in comparison to both pregnant women with impaired glucose tolerance and uncomplicated pregnant subjects 32 . In a study assessing the thiol‐disulfide homeostasis in breast cancer, significant decrease in serum native thiol level was observed in breast cancer patients, along with an enhancement in serum disulfide level, suggesting a role of TDH in the pathogenesis of breast cancer 33 .…”

“…uncomplicated pregnant subjects. 32 In a study assessing the thioldisulfide homeostasis in breast cancer, significant decrease in serum native thiol level was observed in breast cancer patients, along with an enhancement in serum disulfide level, suggesting a role of TDH in the pathogenesis of breast cancer. 33 We showed that the disulfidptosis score was relatively higher in the epithelial and immune cells, compared to stromal cells in breast cancer.…”

The effect of slow-release vaginal dinoprostone on maternal and fetal oxidative stress in term pregnancies complicated by oligohydramnios: Prospective cohort study

“…OS has been examined in cases of fibroids, assisted reproduction, pregnancy loss, gestational diabetes, preeclampsia, IUGR, nuchal cord, and fetal death. [9][10][11][12][13][14][15] Thiols (RSH) are a class of organic compounds that can undergo oxidation reactions via oxidants and form disulfide bonds. Under conditions of OS, dynamic thiol/disulfide homeostasis is maintained, and it plays critical roles in antioxidant protection, detoxification, signal transduction, apoptosis, regulation of enzymatic activity, transcription factors, and cellular signaling mechanisms.…”