Altered gene expression in CHO cells following polyamine starvation

Roca, Berta Capella; Doolan, Padraig; Barron, Niall; O’Neill, Fiona; Clynes, Martin

doi:10.1007/s10529-020-02841-0

Cited by 4 publications

(2 citation statements)

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“…On the other hand, the transcriptional level of transferrin receptor was downregulated, further suggesting that polyamines may promote cell proliferation by regulating the cell cycle and stimulating cellular iron absorption. [105] In addition to yeast extracts, other probiotics have also demonstrated the capability to augment the proliferative activity of intestinal epithelial cells. Notably, studies have revealed that Bifidobacterium longum lysates possess the potential to hinder tumor necrosis factor-induced apoptosis and injury in intestinal epithelial cells.…”

Section: Promote Cell Growth and Proliferationmentioning

confidence: 99%

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Advances in serum‐free media for CHO cells: From traditional serum substitutes to microbial‐derived substances

Zhang,

Zhao,

et al. 2024

Biotechnology Journal

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The Chinese hamster ovary (CHO) cell is an epithelial‐like cell that produces proteins with post‐translational modifications similar to human glycosylation. It is widely used in the production of recombinant therapeutic proteins and monoclonal antibodies. Culturing CHO cells typically requires the addition of a certain proportion of fetal bovine serum (FBS) to maintain cell proliferation and passaging. However, serum is characterized by its complex composition, batch‐to‐batch variability, high cost, and potential risk of exogenous contaminants such as mycoplasma and viruses, which impact the purity and safety of the synthesized proteins. Therefore, search for serum alternatives and development of serum‐free media for CHO‐based protein biomanufacturing are of great significance. This review systematically summarizes the application advantages of CHO cells and strategies for high‐density expression. It highlights the developmental trends of serum substitutes from human platelet lysates to animal‐free extracts and microbial‐derived substances and elucidates the mechanisms by which these substitutes enhance CHO cell culture performance and recombinant protein production, aiming to provide theoretical guidance for exploring novel serum alternatives and developing serum‐free media for CHO cells.

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Section: Promote Cell Growth and Proliferationmentioning

confidence: 99%

Section: Mechanisms Of Sfm On Cell Growth and Recombinant Protein Pro...mentioning

confidence: 99%