Altered expression of heat shock protein 110 (HSP110), hypoxia up- regulated 1 (HYOU1) and translationally controled tumor protein (TCTP) during colorectal cancer progression

The Role of Translationally Controlled Tumor Protein in Tumor Growth and Metastasis of Colon Adenocarcinoma Cells

“…The liver is the most frequent site of metastases. , Special markers for early diagnosis and target for effective therapies of malignancies are imperative needed. On the basis of the recent prognosis research data, TCTP was suggested as a new predictive marker for better understanding of CRC progression involved in tumor metastasis . Moreover, we found that the TCTP expression levels in the high metastatic potential colon cancer cell lines (LoVo, SW620) are higher than those in the relatively lower metastatic potential colon cancer cell lines (LS174T, SW480) as this might explain the importance of TCTP in colon cancer progression (Figure A).…”

“…On the basis of the recent prognosis research data, TCTP was suggested as a new predictive marker for better understanding of CRC progression involved in tumor metastasis. 7 Moreover, we found that the TCTP expression levels in the high metastatic potential colon cancer cell lines (LoVo, SW620) are higher than those in the relatively lower metastatic potential colon cancer cell lines (LS174T, SW480) as this might explain the importance of TCTP in colon cancer progression (Figure 1A). TCTP has been implicated in important cellular processes, such as cell growth, cell cycle progression, and malignant transformation and in the protection of cells against various stress conditions and apoptosis.…”

“…The expression levels of TCTP were also found to be associated with Caco-2 differentiation by proteomics approach . On the basis of recently prognosis studies, TCTP has the potential to be identified as a new predictive marker for better understanding CRC progression involved in metastasis; however, the TCTP biological characteristics in colorectal cancer progression still remained to be elucidated. Double-stranded RNA-mediated interference (RNAi) has recently emerged as a powerful genetic tool to silence gene expression in multiple organisms and has been widely used for gene function analysis. , Small interfering RNA (siRNA) expression mediated by specific plasmids enables efficient and specific suppression of target gene expression in mammalian cells with maintenance of stable loss-of-function phenotypes .…”

iTRAQ-Based Membrane Proteomics Reveals Plasma Membrane Proteins Change During HepaRG Cell Differentiation

“…IPA pathway analysis further identified 11 proteins of particular interest, as evidenced by their involvement in migration. While some of these proteins have been reported previously for other tumor systems, − their differential expressions in HepaRG system are presented here for the first time. While the increase of mRNA (qRT-PCR) correlated with the increase of protein (iTRAQ) for OCLN during HepaRG differentiation, the increase of mRNA unexpectedly correlated with the decrease of protein for MMP-14.…”