Altered expression of Butyrophilin (<i>BTN</i>) and BTN‐like (<i>BTNL</i>) genes in intestinal inflammation and colon cancer

Lebrero‐Fernández, Cristina; Wenzel, Ulf Alexander; Akéus, Paulina; Wang, Ying; Strid, Hans; Simrén, Magnus; Gustavsson, Bengt; Börjesson, Lars; Cardell, Susanna; Öhman, Lena; Quiding‐Järbrink, Marianne; Bas‐Forsberg, Anna

doi:10.1002/iid3.105

Cited by 68 publications

(71 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For real-time PCR analysis, 1.5 μg of total RNA was reverse transcribed into cDNA using a high-capacity RNA-to-cDNA Kit (Applied Biosystems) according to the manufacturer’s instructions. Expression levels of three mucin genes ( MUC2 , MUC4 , and MUC5AC ; TaqMan assay IDs Hs.PT.56a.26485553, Hs.PT.56a.5039491 and Hs.PT.56a.25473826, respectively), relative to the reference gene hypoxanthine phosphoribosyltransferase [ 42 , 43 ] ( HPRT1 ; Hs.PT.39a.22214821, NM_000194.1), were determined using TaqMan probes on the Rotor-Gene 6000 real-time thermal cycler (Corbett Life Science, Concord, Australia). All PCRs (no template controls, untreated, and treated samples) were prepared as triplicate 10 μL reactions as described previously [ 44 ].…”

Section: Methodsmentioning

confidence: 99%

Effect of a Semi-Purified Oligosaccharide-Enriched Fraction from Caprine Milk on Barrier Integrity and Mucin Production of Co-Culture Models of the Small and Large Intestinal Epithelium

et al. 2016

View full text Add to dashboard Cite

Caprine milk contains the highest amount of oligosaccharides among domestic animals, which are structurally similar to human milk oligosaccharides (HMOs). This suggests caprine milk oligosaccharides may offer similar protective and developmental effects to that of HMOs. However, to date, studies using oligosaccharides from caprine milk have been limited. Thus, this study aimed to examine the impact of a caprine milk oligosaccharide-enriched fraction (CMOF) on barrier function of epithelial cell co-cultures of absorptive enterocytes (Caco-2 cells) and mucus-secreting goblet cells (HT29-MTX cells), that more closely simulate the cell proportions found in the small (90:10) and large intestine (75:25). Treatment of epithelial co-cultures with 0.4, 1.0, 2.0 and 4.0 mg/mL of CMOF was shown to have no effect on metabolic activity but did enhance cell epithelial barrier integrity as measured by trans-epithelial electrical resistance (TEER), in a dose-dependent manner. The CMOF at the maximum concentration tested (4.0 mg/mL) enhanced TEER, mucin gene expression and mucin protein abundance of epithelial co-cultures, all of which are essential components of intestinal barrier function.

show abstract

Section: Methodsmentioning

confidence: 99%

Effect of a Semi-Purified Oligosaccharide-Enriched Fraction from Caprine Milk on Barrier Integrity and Mucin Production of Co-Culture Models of the Small and Large Intestinal Epithelium

et al. 2016

View full text Add to dashboard Cite

show abstract

“…[118] Gene expression analysis of biopsies from ulcerative colitis patients showed increased BTN3 and decreased BTNL8 expression. [119] Based on the function of these genes, these changes in expression correlate with a suppressive effect on T cell activation;[119] however, the effect that this has on γδ IEL activation remains to be determined.…”

Section: Addressing the Differences Between Mice And Men: γδ Iels In Ibdmentioning

confidence: 99%

Sentinels at the Frontline: the Role of Intraepithelial Lymphocytes in Inflammatory Bowel Disease

Edelblum

2017

Curr Pharmacol Rep

View full text Add to dashboard Cite

Purpose of review Intestinal mucosal immunity is tightly regulated to ensure effective host defense against invasive microorganisms while limiting the potential for aberrant damage. In inflammatory bowel disease (IBD), an imbalance between effector and regulatory T cell populations results in an uncontrolled inflammatory response to commensal bacteria. Intraepithelial lymphocytes (IEL) are perfectly positioned within the intestinal epithelium to provide the first line of mucosal defense against luminal microbes or rapidly respond to epithelial injury. This review will highlight how IELs promote protective intestinal immunity and discuss the evidence indicating that altered IEL responses contribute to the pathogenesis of IBD. Recent findings Although the role of IELs in mucosal homeostasis has been largely underappreciated, many of the same factors that contribute to the dysregulation of host defense in IBD also adversely affect IELs. For example, IL-23 and the endoplasmic reticulum stress response can enhance IEL lytic activity toward enterocytes. Microbial dysbiosis or defective microbial recognition results in the loss of regulatory IELs, further amplifying these pro-inflammatory effects. Migration of T cells into or within the intraepithelial compartment has a profound effect on their differentiation or effector function demonstrating that IELs are exquisitely sensitive to changes in the local intestinal microenvironment. Summary Enhanced mechanistic insight into the regulation of IEL survival, differentiation and effector function may provide useful tools to modulate IEL surveillance or enhance IEL regulatory function. Elucidation of these processes may result in the development of novel therapeutics to reduce intestinal inflammation and reinforce the mucosal barrier in IBD.

show abstract

“…It has been localized to Peyer's patches of the small intestine as well as epithelial and dendritic cells within gastrointestinal tissue. Altered expression of BTNL2 is associated with inflammatory bowel disease [14]. Mutations in this gene are further associated with other autoimmune conditions including sarcoidosis, rheumatoid arthritis, vitiligo, and psoriasis [15][16][17][18].…”

Section: Discussionmentioning

confidence: 99%

Primary Immunodeficiency with Severe Multi-Organ Immune Dysregulation

Гаврилова

2019

Case Reports in Immunology

View full text Add to dashboard Cite

Polyglandular autoimmune syndrome type 1, also known as autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED), is a rare primary immunodeficiency disorder with multi-organ involvement. Besides for being predisposed to severe life-threatening infections, patients with APECED are also prone to organ impairment secondary to severe autoimmunity. As this is an autosomal recessive disorder, a biallelic mutation in the AIRE gene is responsible for APECED. The author presents a case of APECED with a single AIRE mutation. Whole exome sequencing identified a mutation in the BTNL2 gene that the author suggests may have contributed to the patient’s presentation.

show abstract

Altered expression of Butyrophilin (BTN) and BTN‐like (BTNL) genes in intestinal inflammation and colon cancer

Cited by 68 publications

References 41 publications

Effect of a Semi-Purified Oligosaccharide-Enriched Fraction from Caprine Milk on Barrier Integrity and Mucin Production of Co-Culture Models of the Small and Large Intestinal Epithelium

Effect of a Semi-Purified Oligosaccharide-Enriched Fraction from Caprine Milk on Barrier Integrity and Mucin Production of Co-Culture Models of the Small and Large Intestinal Epithelium

Sentinels at the Frontline: the Role of Intraepithelial Lymphocytes in Inflammatory Bowel Disease

Primary Immunodeficiency with Severe Multi-Organ Immune Dysregulation

Contact Info

Product

Resources

About