Altered Cell Motility and Attachment With Titanium Surface Modifications

Palaiologou, Archontia; Stoute, Diana; Fan, Yuwei; Lallier, Thomas E.

doi:10.1902/jop.2011.100733

Cited by 9 publications

(7 citation statements)

References 35 publications

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“…Moreover, immortalized and/or xenogenic cell lines, which slightly reflect the expected behavior of healthy human cells of the peri-implant tissue, have been used. Other important aspects of cell behavior for a successful tissue integration of implant material, such as cell migration or quality of adhesion, are not the focus of early screening schemes [23,28]. To avoid time-consuming and expensive in vivo investigations of ultimately deficient materials, a more reliable in vitro examination is required in order to detect any biocompatibility issues during the initial in vitro screening.…”

Section: Discussionmentioning

confidence: 99%

“…It is already known that cells from mice have a seven times higher metabolic rate than those from humans and tumor cells differ in size, proliferation, and metabolism from healthy counterparts, which in part might be an explanation for the described discrepancies [68,69]. However, due to a better availability and an easier cultivation of primary fibroblasts compared to primary osteoblasts, several studies compared primary fibroblasts with immortalized osteoblasts when analyzing the cell-specific effects of an implant surface [23,70,71]. As the results of this study illustrate, this approach can lead to false conclusions.…”

Section: Discussionmentioning

confidence: 99%

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Cell Type-Specific Adhesion and Migration on Laser-Structured Opaque Surfaces

Schaeske

Fadeeva

Schlie-Wolter

et al. 2020

IJMS

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Cytocompatibility is essential for implant approval. However, initial in vitro screenings mainly include the quantity of adherent immortalized cells and cytotoxicity. Other vital parameters, such as cell migration and an in-depth understanding of the interaction between native tissue cells and implant surfaces, are rarely considered. We investigated different laser-fabricated spike structures using primary and immortalized cell lines of fibroblasts and osteoblasts and included quantification of the cell area, aspect ratio, and focal adhesions. Furthermore, we examined the three-dimensional cell interactions with spike topographies and developed a tailored migration assay for long-term monitoring on opaque materials. While fibroblasts and osteoblasts on small spikes retained their normal morphology, cells on medium and large spikes sank into the structures, affecting the composition of the cytoskeleton and thereby changing cell shape. Up to 14 days, migration appeared stronger on small spikes, probably as a consequence of adequate focal adhesion formation and an intact cytoskeleton, whereas human primary cells revealed differences in comparison to immortalized cell lines. The use of primary cells, analysis of the cell–implant structure interaction as well as cell migration might strengthen the evaluation of cytocompatibility and thereby improve the validity regarding the putative in vivo performance of implant material.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Cell Type-Specific Adhesion and Migration on Laser-Structured Opaque Surfaces

Schaeske

Fadeeva

Schlie-Wolter

et al. 2020

IJMS

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“…Our results demonstrated that NaTi surface might provide a promising environment for PDL ligament-anchored implants in the future by regulating the proliferation rate, morphology and differentiation of PDL cells. Cell culture studies are valuable to investigate the biological interaction of new titanium surfaces/cells and provide to gain an initial insight regarding cell morphology, adhesion, migration, proliferation and differentiation on implant surfaces [7,53]. However, cell culture studies can not simulate the dynamic environment of bone/implant surface and in vivo performance of modified/novel implant surfaces should be confirmed in animal models and subsequent clinical trials.…”

Section: Discussionmentioning

confidence: 99%

Periodontal ligament cell behavior on different titanium surfaces

Hakkı

Korkusuz²,

Puralı

et al. 2012

Acta Odontologica Scandinavica

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“…PDL also plays an important role in the movement of teeth and in maintaining new homeostasis. [1][2][3] Technology that facilitates the regeneration of tooth must receive considerable attention, especially in prosthodontics. [4][5][6][7] In this case, the conventional regenerative dentistry has been developing stem cell technology, scaffolds and growth factors to produce biotechnological artificial teeth.…”

Section: Research Report Introductionmentioning

confidence: 99%

The effect of 1,25-dihydroxyvitamin D3 on MSX2 gene expression during tooth and alveolar bone development

Ruspita¹

2015

Dent. J. (Majalah Kedokteran Gigi)

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Background: 1,25-dihydroxyvitamin D3 has been proven to be able to control the formation and biomineralization of tissue through a regulatory gene. A previous research even showed that a cell responsible for the formation of the enamel, dentin and bone was the target of 1,25-dihydroxivitamin D3. Purpose: This research was aimed to determine the role of 1,25-dihydroxyvitamin D3 in vivo in the development of teeth and alveolar bone tissue by analyzing MSX2 gene expression as a gene marker responsible for the growth and development of enamel, dentin, tooth root and alveolar bone. Methods: Samples used for RT-PCR analysis were total RNA of insisivus teeth and alveolar bone derived from mice. RT-PCR analysis was conducted by using primer-specific gene, MSX2. Primer gene, GAPDH, was also used as an internal control. Five hundred nanograms of total RNA were used as a template for PCR. Semi quantitative results of PCR were quantified by using ImageJ software. results: RT-PCR analysis showed that the expression level of MSX2 was enhanced in the samples of teeth and alveolar bone treated with 1,25 dihydroxyvitamin D3. The increasing of MSX2 expression significantly occurred in alveolar bone samples. Conclusion: It can be concluded that 1,25 dihydroxyvitamin D3 could enhance MSX2 expression as a marker of the development of teeth and alveolar bone tissue. Therefore, 1,25-D3 dihydroxyvitamin is expected to be used as an agent to help the regeneration of teeth and bone tissue.

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Altered Cell Motility and Attachment With Titanium Surface Modifications

Cited by 9 publications

References 35 publications

Cell Type-Specific Adhesion and Migration on Laser-Structured Opaque Surfaces

Cell Type-Specific Adhesion and Migration on Laser-Structured Opaque Surfaces

Periodontal ligament cell behavior on different titanium surfaces

The effect of 1,25-dihydroxyvitamin D3 on MSX2 gene expression during tooth and alveolar bone development

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