Altered bacterial aggregation and adherence associated with changes in rat parotid-gland salivary proteins induced in vivo by β-adrenergic stimulation

Saliva composition and caries development in asthmatic patients treated with β2‐adrenoceptor agonists: a 4‐year follow‐up study

“…The output of the antibacterial components was lower in the asthma patients. This should favor both bacterial colonization and plaque growth (19,20).…”

Saliva-Bacterium Interactions in Oral Microbial Ecology

“…There is little evidence that PRPs bind to the surface of bacteria from the solution phase . One study, however, has reported an association between isoproteranol-induced secretion of a prolinerich protein in rat saliva and agglutination of mutans streptococci, S. sanguis and A. viscosus (Kousvelari et al, 1988). There have been several reports, however, suggesting a role for PRPs in the adhesion of bacteria such as A. viscosus (Clark et al, 1989;Gibbons and Hay, 1989;Stromberg et al, 1992), S. mutans , and S. gordonii to tooth surfaces.…”

Adherence of Microorganisms to Rat Salivary Pellicles

“…Hu man acidic PRPs have been shown to promote adhesion of bacteria such as A. viscosus [Gibbons and Hay, 1988b] and S. gordonii [Gibbons et al, 1991] to apatitic surfaces. A marked reduction in adherence o f streptococcal strains to HA coated with saliva from isoproterenol-treated rats has been reported [Kousvelari et al, 1988], Isoproterenol-treat ed saliva contains significantly less acidic PRP than untreat ed saliva. Rat submandibular gland mucin (97 kD) and rat sublingual gland mucin (2,000 kD) were not detected in rsHA by Western blot analysis, however, high-molecularweight glycoproteins were detected by Alcian blue staining and enhancement of Alcian blue by silver stain.…”

“…The protein composition of rat saliva has been examined [Keller et al, 1975;Johnson, 1984]; however, characterization of the interactions of rat salivary proteins and bacterial adherence have not been extensively addressed. Rat salivary acidic PRP was shown to be the pre dominant protein adsorbed to HA [Kousvelari et al, 1988],…”

“…We were able to identify few studies in which in vitro pellicles were formed using rat saliva. HA coated with rat saliva (rsHA) bound fewer Streptococcus salivarius HB than did HA [Weerkamp and McBride, 1980], Pellicles formed from rat parotid saliva supported the adherence of strains of Streptococcus and Actinomyces, although the amount of adherence was influenced by chronic treatment of the rats with isoproterenol and propranolol [Kousvelari et al, 1988], Studies of human salivary proteins have shown that cer tain bacterial strains adhere to specific proteins. For exam ple, Murray et al [1992] found that some oral streptococci can bind specifically to certain salivary glycoproteins.…”